Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes.

Abstract:

BACKGROUND:Physiological long term adaptation of pancreatic beta cells is driven by stimuli such as glucose and incretin hormones acting via cAMP (e.g. GLP-1) and involves regulated gene expression. Several rapidly inducible immediate-early genes (IEGs) have been identified in beta cells. Many of these IEGs code for transcription factors and have the potential to control the transcription of downstream target genes likely involved in long term cellular adaptation. The identity of these target genes has not been determined, and the sequence of events occurring during beta cell adaptation is still unclear. RESULTS:We have developed a microarray-based strategy for the systematic search of targets. In Min6 insulin-secreting cells, we identified 592 targets and 1278 IEGs responding to a co-stimulation with glucose and cAMP. Both IEGs and targets were involved in a large panel of functions, including those important to beta cell physiology (metabolism, secretion). Nearly 200 IEGs were involved in signaling and transcriptional regulation. To find specific examples of the regulatory link between IEGs and targets, target promoter sequences were analyzed in silico. Statistically significant over-representation of AP-1 response elements notably suggested an important role for this transcription factor, which was experimentally verified. Indeed, cell stimulation altered expression of IEG-encoded components of the AP-1 complex, activating AP-1-dependent transcription. Loss and gain-of-function experiments furthermore allowed to validate a new AP-1 regulated gene (sulfiredoxin) among the targets. AP-1 and sulfiredoxin are sequentially induced also in primary cells from rat islets of Langerhans. CONCLUSION:By identifying IEGs and their downstream targets, this study brings a comprehensive description of the transcriptional response occurring after beta cell stimulation, as well as new mechanistic insights concerning the AP-1 transcription factor.

journal_name

BMC Mol Biol

journal_title

BMC molecular biology

authors

Glauser DA,Brun T,Gauthier BR,Schlegel W

doi

10.1186/1471-2199-8-54

subject

Has Abstract

pub_date

2007-06-22 00:00:00

pages

54

issn

1471-2199

pii

1471-2199-8-54

journal_volume

8

pub_type

杂志文章
  • The major transcription initiation site of the p27Kip1 gene is conserved in human and mouse and produces a long 5'-UTR.

    abstract:BACKGROUND:The cyclin-dependent kinase inhibitor p27Kip1 is essential for proper control of cell cycle progression. The levels of p27Kip1 are regulated by several mechanisms including transcriptional and translational controls. In order to delineate the molecular details of these regulatory mechanisms it is important t...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-2-12

    authors: Coleman J,Hawkinson M,Miskimins R,Miskimins WK

    更新日期:2001-01-01 00:00:00

  • Discriminating nucleosomes containing histone H2A.Z or H2A based on genetic and epigenetic information.

    abstract:BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-18

    authors: Gervais AL,Gaudreau L

    更新日期:2009-03-04 00:00:00

  • RAD51 paralogs promote homology-directed repair at diversifying immunoglobulin V regions.

    abstract:BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-98

    authors: Ordinario EC,Yabuki M,Handa P,Cummings WJ,Maizels N

    更新日期:2009-10-28 00:00:00

  • Selection of reference genes for normalisation of real-time RT-PCR in brain-stem death injury in Ovis aries.

    abstract:BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-72

    authors: Passmore M,Nataatmadja M,Fraser JF

    更新日期:2009-07-23 00:00:00

  • Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line.

    abstract:BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-11-58

    authors: Yan B,Li D,Gou K

    更新日期:2010-08-13 00:00:00

  • Transpositionally active episomal hAT elements.

    abstract:BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-108

    authors: O'Brochta DA,Stosic CD,Pilitt K,Subramanian RA,Hice RH,Atkinson PW

    更新日期:2009-12-14 00:00:00

  • Characterization of a novel bifunctional mutant of staphylokinase with platelet-targeted thrombolysis and antiplatelet aggregation activities.

    abstract:BACKGROUND:Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active platelet binding ability, and at the same time the rethrombosis complication after successful recana...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-8-88

    authors: Chen H,Mo W,Su H,Zhang Y,Song H

    更新日期:2007-10-07 00:00:00

  • Phylogeny and expression of carbonic anhydrase-related proteins.

    abstract:BACKGROUND:Carbonic anhydrases (CAs) are found in many organisms, in which they contribute to several important biological processes. The vertebrate alpha-CA family consists of 16 subfamilies, three of which (VIII, X and XI) consist of acatalytic proteins. These are named carbonic anhydrase related proteins (CARPs), an...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-11-25

    authors: Aspatwar A,Tolvanen ME,Parkkila S

    更新日期:2010-03-31 00:00:00

  • Identification of ColR binding consensus and prediction of regulon of ColRS two-component system.

    abstract:BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-46

    authors: Kivistik PA,Kivi R,Kivisaar M,Hõrak R

    更新日期:2009-05-16 00:00:00

  • What is normal? Next generation sequencing-driven analysis of the human circulating miRNAOme.

    abstract:BACKGROUND:MicroRNAs (miRNAs) are short non-protein-coding RNA species that have a regulatory function in modulating protein translation and degradation of specific mRNAs. MicroRNAs are estimated to target approximately 60% of all human mRNAs and are associated with the regulation of all physiological processes. Simila...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/s12867-016-0057-9

    authors: Tonge DP,Gant TW

    更新日期:2016-02-09 00:00:00

  • Comparative in silico analysis identifies bona fide MyoD binding sites within the Myocyte stress 1 gene promoter.

    abstract:BACKGROUND:Myocyte stress 1 (MS1) is a striated muscle actin binding protein required for the muscle specific activity of the evolutionary ancient myocardin related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathway. To date, little is known about the molecular mechanisms that govern skelet...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-9-50

    authors: Ounzain S,Dacwag CS,Samani NJ,Imbalzano AN,Chong NW

    更新日期:2008-05-19 00:00:00

  • Relative transcript quantification by quantitative PCR: roughly right or precisely wrong?

    abstract:BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-6-10

    authors: Skern R,Frost P,Nilsen F

    更新日期:2005-04-26 00:00:00

  • Transcription of the rat testis-specific Rtdpoz-T1 and -T2 retrogenes during embryo development: co-transcription and frequent exonisation of transposable element sequences.

    abstract:BACKGROUND:Retrotransposition is an important evolutionary force for the creation of new and potentially functional intronless genes which are collectively called retrogenes. Many retrogenes are expressed in the testis and the gene products have been shown to actively participate in spermatogenesis and other unique fun...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-74

    authors: Huang CJ,Lin WY,Chang CM,Choo KB

    更新日期:2009-07-25 00:00:00

  • SPRR2A enhances p53 deacetylation through HDAC1 and down regulates p21 promoter activity.

    abstract:BACKGROUND:Small proline rich protein (SPRR) 2A is one of 14 SPRR genes that encodes for a skin cross-linking protein, which confers structural integrity to the cornified keratinocyte cell envelope. New evidence, however, shows that SPRR2A is also a critical stress and wound repair modulator: it enables a variety of ba...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-13-20

    authors: Mizuguchi Y,Specht S,Lunz JG 3rd,Isse K,Corbitt N,Takizawa T,Demetris AJ

    更新日期:2012-06-25 00:00:00

  • TRPC1 transcript variants, inefficient nonsense-mediated decay and low up-frameshift-1 in vascular smooth muscle cells.

    abstract:BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-12-30

    authors: Dedman AM,Majeed Y,Tumova S,Zeng F,Kumar B,Munsch C,Bateson AN,Wittmann J,Jäck HM,Porter KE,Beech DJ

    更新日期:2011-07-12 00:00:00

  • Genome-wide identification of brain miRNAs in response to high-intensity intermittent swimming training in Rattus norvegicus by deep sequencing.

    abstract:BACKGROUND:Physical exercise can improve brain function by altering brain gene expression. The expression mechanisms underlying the brain's response to exercise still remain unknown. miRNAs as vital regulators of gene expression may be involved in regulation of brain genes in response to exercise. However, as yet, very...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/s12867-019-0120-4

    authors: Zhao Y,Zhang A,Wang Y,Hu S,Zhang R,Qian S

    更新日期:2019-01-15 00:00:00

  • Identification and characterization of CCAAT/Enhancer Binding proteindelta (C/EBPdelta) target genes in G0 growth arrested mammary epithelial cells.

    abstract:BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-9-83

    authors: Zhang Y,Liu T,Yan P,Huang T,Dewille J

    更新日期:2008-10-01 00:00:00

  • Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymerase.

    abstract:BACKGROUND:Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. It is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins. The biochemical proper...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-8-59

    authors: Kim YG,Yoo JS,Kim JH,Kim CM,Oh JW

    更新日期:2007-07-11 00:00:00

  • Translation initiation region sequence preferences in Escherichia coli.

    abstract:BACKGROUND:The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably the most abundant class of enhancers contains A/U-rich sequences. We have tested the influence of SD sequence length and the presence of enhancers on the efficiency o...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-8-100

    authors: Vimberg V,Tats A,Remm M,Tenson T

    更新日期:2007-10-31 00:00:00

  • Alternative splicing of the neurofibromatosis type 1 pre-mRNA is regulated by the muscleblind-like proteins and the CUG-BP and ELAV-like factors.

    abstract:BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-13-35

    authors: Fleming VA,Geng C,Ladd AN,Lou H

    更新日期:2012-12-10 00:00:00

  • Ataxia telangiectasia mutated (ATM) interacts with p400 ATPase for an efficient DNA damage response.

    abstract:BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/s12867-016-0075-7

    authors: Smith RJ,Savoian MS,Weber LE,Park JH

    更新日期:2016-11-04 00:00:00

  • The human RPS4 paralogue on Yq11.223 encodes a structurally conserved ribosomal protein and is preferentially expressed during spermatogenesis.

    abstract:BACKGROUND:The Y chromosome of mammals is particularly prone to accumulate genes related to male fertility. However, the high rate of molecular evolution on this chromosome predicts reduced power to the across-species comparative approach in identifying male-specific genes that are essential for sperm production in hum...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-11-33

    authors: Lopes AM,Miguel RN,Sargent CA,Ellis PJ,Amorim A,Affara NA

    更新日期:2010-05-07 00:00:00

  • Checkpoint effects and telomere amplification during DNA re-replication in fission yeast.

    abstract:BACKGROUND:Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which check...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-8-119

    authors: Mickle KL,Oliva A,Huberman JA,Leatherwood J

    更新日期:2007-12-21 00:00:00

  • Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2.

    abstract:BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-9-11

    authors: Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo P

    更新日期:2008-01-23 00:00:00

  • Alternative splicing and differential subcellular localization of the rat FGF antisense gene product.

    abstract:BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-9-10

    authors: Zhang SC,MacDonald KA,Baguma-Nibasheka M,Geldenhuys L,Casson AG,Murphy PR

    更新日期:2008-01-23 00:00:00

  • Structural and functional analysis of four non-coding Y RNAs from Chinese hamster cells: identification, molecular dynamics simulations and DNA replication initiation assays.

    abstract:BACKGROUND:The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no information is available about Y RNAs in Chinese hamster cells, which have already been used to detect repli...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/s12867-015-0053-5

    authors: de Lima Neto QA,Duarte Junior FF,Bueno PS,Seixas FA,Kowalski MP,Kheir E,Krude T,Fernandez MA

    更新日期:2016-01-05 00:00:00

  • Rad9 modulates the P21WAF1 pathway by direct association with p53.

    abstract:BACKGROUND:Previous studies suggest that human RAD9 (hRad9), encoding a DNA damage checkpoint molecule, which is frequently amplified in epithelial tumor cells of breast, lung, head and neck cancer, participates in regulation of the tumor suppressor p53-dependent transactivation of pro-survival P21WAF1. This study exam...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-8-37

    authors: Ishikawa K,Ishii H,Murakumo Y,Mimori K,Kobayashi M,Yamamoto K,Mori M,Nishino H,Furukawa Y,Ichimura K

    更新日期:2007-05-21 00:00:00

  • Deficiency of Pten accelerates mammary oncogenesis in MMTV-Wnt-1 transgenic mice.

    abstract:BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently f...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-2-2

    authors: Li Y,Podsypanina K,Liu X,Crane A,Tan LK,Parsons R,Varmus HE

    更新日期:2001-01-01 00:00:00

  • Genetic correction of splice site mutation in purified and enriched myoblasts isolated from mdx5cv mice.

    abstract:BACKGROUND:Duchenne Muscular Dystrophy (DMD) is an X-linked genetic disorder that results in the production of a dysfunctional form of the protein, dystrophin. The mdx5cv mouse is a model of DMD in which a point mutation in exon 10 of the dystrophin gene creates an artificial splice site. As a result, a 53 base pair de...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/1471-2199-10-15

    authors: Maguire K,Suzuki T,DiMatteo D,Parekh-Olmedo H,Kmiec E

    更新日期:2009-02-23 00:00:00

  • Efficient 5'-3' DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicus.

    abstract:BACKGROUND:ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hype...

    journal_title:BMC molecular biology

    pub_type: 杂志文章

    doi:10.1186/s12867-015-0030-z

    authors: Huang Q,Liu L,Liu J,Ni J,She Q,Shen Y

    更新日期:2015-02-14 00:00:00