Abstract:
BACKGROUND:Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS:Rat RGC-5 cells were cultured in plates and H2O2 was added to induce oxidative stress. TUNEL assay and qRT-PCR showed H2O2 induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected H2O2 induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis. CONCLUSION:Down-regulating miR-100 is an effective method to protect H2O2 induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Kong N,Lu X,Li Bdoi
10.1186/s12867-014-0025-1subject
Has Abstractpub_date
2014-11-18 00:00:00pages
25issn
1471-2199pii
s12867-014-0025-1journal_volume
15pub_type
杂志文章abstract:BACKGROUND:Small nucleolar RNAs (snoRNAs) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs (rRNAs) and small nuclear RNAs (snRNAs). In recent years, more and more snoRNAs have been found to play novel roles in mRNA regulation, such as pre-mRNA splicing or RNA editing. In our previous study, ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-019-0128-9
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Visceral leishmaniasis caused by members of the Leishmania donovani complex is often fatal in the absence of treatment. Research has been hampered by the lack of good laboratory models and tools for genetic manipulation. In this study, we have characterised a L. infantum line (JPCM5) that was isolated from a...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-7-42
更新日期:2006-11-13 00:00:00
abstract:BACKGROUND:The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The present study aimed to identify reference genes as internal controls for gene expression studies by RT-qPC...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-13-22
更新日期:2012-06-29 00:00:00
abstract:BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-78
更新日期:2007-09-17 00:00:00
abstract:BACKGROUND:The cyclin-dependent kinase inhibitor p27Kip1 is essential for proper control of cell cycle progression. The levels of p27Kip1 are regulated by several mechanisms including transcriptional and translational controls. In order to delineate the molecular details of these regulatory mechanisms it is important t...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-2-12
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-46
更新日期:2009-05-16 00:00:00
abstract:BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-112
更新日期:2007-12-19 00:00:00
abstract:BACKGROUND:Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purificatio...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-15-7
更新日期:2014-04-03 00:00:00
abstract:BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-6-2
更新日期:2005-01-19 00:00:00
abstract:BACKGROUND:Previous studies suggest that human RAD9 (hRad9), encoding a DNA damage checkpoint molecule, which is frequently amplified in epithelial tumor cells of breast, lung, head and neck cancer, participates in regulation of the tumor suppressor p53-dependent transactivation of pro-survival P21WAF1. This study exam...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-37
更新日期:2007-05-21 00:00:00
abstract:BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-18
更新日期:2009-03-04 00:00:00
abstract:BACKGROUND:Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human embryonic stem (hES) cells, differentiation itself introduces chan...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-14-21
更新日期:2013-09-12 00:00:00
abstract:BACKGROUND:Carbonic anhydrases (CAs) are found in many organisms, in which they contribute to several important biological processes. The vertebrate alpha-CA family consists of 16 subfamilies, three of which (VIII, X and XI) consist of acatalytic proteins. These are named carbonic anhydrase related proteins (CARPs), an...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-25
更新日期:2010-03-31 00:00:00
abstract:BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-69
更新日期:2010-09-14 00:00:00
abstract:BACKGROUND:ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hype...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0030-z
更新日期:2015-02-14 00:00:00
abstract:BACKGROUND:Double-strand breakage of chromosomal DNA is obviously a serious threat to cells because various activities of the chromosome depend on its integrity. However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, p...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-4-5
更新日期:2003-04-28 00:00:00
abstract:BACKGROUND:Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active platelet binding ability, and at the same time the rethrombosis complication after successful recana...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-88
更新日期:2007-10-07 00:00:00
abstract:BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-13-35
更新日期:2012-12-10 00:00:00
abstract:BACKGROUND:Retrotransposition is an important evolutionary force for the creation of new and potentially functional intronless genes which are collectively called retrogenes. Many retrogenes are expressed in the testis and the gene products have been shown to actively participate in spermatogenesis and other unique fun...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-74
更新日期:2009-07-25 00:00:00
abstract:BACKGROUND:Naturally occurring tRNAs contain numerous modified nucleosides. They are formed by enzymatic modification of the primary transcripts during the complex RNA maturation process. In model organisms Escherichia coli and Saccharomyces cerevisiae most enzymes involved in this process have been identified. Interes...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-7-23
更新日期:2006-07-18 00:00:00
abstract:BACKGROUND:Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regula...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-15-10
更新日期:2014-05-25 00:00:00
abstract:BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-27
更新日期:2007-04-12 00:00:00
abstract:BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0075-7
更新日期:2016-11-04 00:00:00
abstract:BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-72
更新日期:2009-07-23 00:00:00
abstract:BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-7-45
更新日期:2006-11-29 00:00:00
abstract:BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-95
更新日期:2009-10-16 00:00:00
abstract:BACKGROUND:Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. It is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins. The biochemical proper...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-59
更新日期:2007-07-11 00:00:00
abstract:BACKGROUND:The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no information is available about Y RNAs in Chinese hamster cells, which have already been used to detect repli...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0053-5
更新日期:2016-01-05 00:00:00
abstract:BACKGROUND:Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we descr...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-018-0110-y
更新日期:2018-07-27 00:00:00
abstract:BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-70
更新日期:2008-08-01 00:00:00