Abstract：
BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter GATA elements in skeletal muscle cells. RESULTS:Mutation or deletion of GATA elements induces a strong transcriptional activation of the cTnI promoter in regenerating skeletal muscle and in cultured skeletal muscle cells. Electrophoretic mobility shift assays show that proteins present in nuclear extracts of C2C12 muscle cells bind the GATA motifs present in the cTnI promoter. However, GATA protein complex formation is neither reduced nor supershifted by antibodies specific for GATA-2, -3 and -4, the only GATA transcripts present in muscle cells. CONCLUSION:These findings indicate that the cTnI gene promoter is repressed in skeletal muscle cells by GATA-like factors and open the way to further studies aimed at identifying these factors.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Di Lisi R,Picard A,Ausoni S,Schiaffino Sdoi
10.1186/1471-2199-8-78subject
Has Abstractpub_date
2007-09-17 00:00:00pages
78issn
1471-2199pii
1471-2199-8-78journal_volume
8pub_type
杂志文章abstract：BACKGROUND:Oxidative stress can induce cell injury in vascular endothelial cells, which is the initial event in the development of atherosclerosis. Although quantitative real-time polymerase chain reaction (qRT-PCR) has been widely used in gene expression studies in oxidative stress injuries, using carefully validated ...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/s12867-017-0086-z
更新日期：2017-04-05 00:00:00
abstract：BACKGROUND:Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purificatio...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-15-7
更新日期：2014-04-03 00:00:00
abstract：BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-11-69
更新日期：2010-09-14 00:00:00
abstract：BACKGROUND:Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference ("housekeeping") gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping ...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-9-102
更新日期：2008-11-12 00:00:00
abstract：BACKGROUND:Improving the treatment of renal cell carcinoma (RCC) will depend on the development of better biomarkers for predicting disease progression and aiding the design of appropriate therapies. One such marker may be fatty acid binding protein 7 (FABP7), also known as B-FABP and BLBP, which is expressed normally ...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-12-31
更新日期：2011-07-19 00:00:00
abstract：BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-8-112
更新日期：2007-12-19 00:00:00
abstract：BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-108
更新日期：2009-12-14 00:00:00
abstract：BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-18
更新日期：2009-03-04 00:00:00
abstract：BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-11-58
更新日期：2010-08-13 00:00:00
abstract：BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-72
更新日期：2009-07-23 00:00:00
abstract：BACKGROUND:Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. RESULTS:Insertion of a TEV protease cleavage site into Cdc23 allows i...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-6-13
更新日期：2005-06-07 00:00:00
abstract：BACKGROUND:Double-strand breakage of chromosomal DNA is obviously a serious threat to cells because various activities of the chromosome depend on its integrity. However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, p...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-4-5
更新日期：2003-04-28 00:00:00
abstract：BACKGROUND:Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-12-32
更新日期：2011-08-01 00:00:00
abstract：BACKGROUND:RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal pathway. However, the role of RA and Stra8 in germ cell meiotic initiation in teleosts is poorly ch...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-14-11
更新日期：2013-05-22 00:00:00
abstract：BACKGROUND:Senescence marker protein-30 (SMP30), whose expression declines during aging in rat liver, has been proposed as an important aging marker. Besides apoptosis, SMP30 also protects cells against various other injuries by enhancement of membrane calcium-pump activity. The mechanism of this differential gene expr...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-9-43
更新日期：2008-04-29 00:00:00
abstract：BACKGROUND:Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its e...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-11-98
更新日期：2010-12-11 00:00:00
abstract：BACKGROUND:Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as their expression is a...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-99
更新日期：2009-10-30 00:00:00
abstract：BACKGROUND:Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human embryonic stem (hES) cells, differentiation itself introduces chan...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-14-21
更新日期：2013-09-12 00:00:00
abstract：BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-8-27
更新日期：2007-04-12 00:00:00
abstract：BACKGROUND:Duchenne Muscular Dystrophy (DMD) is an X-linked genetic disorder that results in the production of a dysfunctional form of the protein, dystrophin. The mdx5cv mouse is a model of DMD in which a point mutation in exon 10 of the dystrophin gene creates an artificial splice site. As a result, a 53 base pair de...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-15
更新日期：2009-02-23 00:00:00
abstract：BACKGROUND:Myocyte stress 1 (MS1) is a striated muscle actin binding protein required for the muscle specific activity of the evolutionary ancient myocardin related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathway. To date, little is known about the molecular mechanisms that govern skelet...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-9-50
更新日期：2008-05-19 00:00:00
abstract：BACKGROUND:The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or of limited use. RESU...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/s12867-016-0060-1
更新日期：2016-03-15 00:00:00
abstract：BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-98
更新日期：2009-10-28 00:00:00
abstract：BACKGROUND:Sequence periodicity with a period close to the DNA helical repeat is a very basic genomic property. This genomic feature was demonstrated for many prokaryotic genomes. The Escherichia coli sequences display the period close to 11 base pairs. RESULTS:Here we demonstrate that practically only ApA/TpT dinucle...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-5-14
更新日期：2004-08-26 00:00:00
abstract：BACKGROUND:Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we descr...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/s12867-018-0110-y
更新日期：2018-07-27 00:00:00
abstract：BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/s12867-016-0075-7
更新日期：2016-11-04 00:00:00
abstract：BACKGROUND:Cyclic AMP-dependent protein kinase (PKA) plays a central role in regulation of energy metabolism. Upon stimulation of testicular Sertoli cells by follicle stimulating hormone (FSH), glycolysis is activated to increase the production of nutrients for the germ cells, and a new regulatory subunit of cAMP-depen...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-3-10
更新日期：2002-06-21 00:00:00
abstract：BACKGROUND:Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which check...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-8-119
更新日期：2007-12-21 00:00:00
abstract：BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-10-97
更新日期：2009-10-25 00:00:00
abstract：BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...
journal_title：BMC molecular biology
pub_type： 杂志文章
doi：10.1186/1471-2199-9-10
更新日期：2008-01-23 00:00:00
