Single and combinatorial chromatin coupling events underlies the function of transcript factor Krüppel-like factor 11 in the regulation of gene networks.

abstract：BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...

journal_title：BMC molecular biology

authors： Bondino HG,Valle EM

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...

journal_title：BMC molecular biology

authors： O'Brochta DA,Stosic CD,Pilitt K,Subramanian RA,Hice RH,Atkinson PW

更新日期：2009-12-14 00:00:00

abstract：BACKGROUND:The cyclin-dependent kinase inhibitor p27Kip1 is essential for proper control of cell cycle progression. The levels of p27Kip1 are regulated by several mechanisms including transcriptional and translational controls. In order to delineate the molecular details of these regulatory mechanisms it is important t...

journal_title：BMC molecular biology

authors： Coleman J,Hawkinson M,Miskimins R,Miskimins WK

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we descr...

journal_title：BMC molecular biology

authors： Brzek A,Cichocka M,Dolata J,Juzwa W,Schümperli D,Raczynska KD

更新日期：2018-07-27 00:00:00

abstract：BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...

journal_title：BMC molecular biology

authors： Kivistik PA,Kivi R,Kivisaar M,Hõrak R

更新日期：2009-05-16 00:00:00

abstract：BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...

journal_title：BMC molecular biology

authors： Jin H,Vacic V,Girke T,Lonardi S,Zhu JK

更新日期：2008-01-14 00:00:00

abstract：BACKGROUND:SRY is the pivotal gene initiating male sex determination in most mammals, but how its expression is regulated is still not understood. In this study we derived novel SRY 5' flanking genomic sequence data from bovine and caprine genomic BAC clones. RESULTS:We identified four intervals of high homology upstr...

journal_title：BMC molecular biology

authors： Ross DG,Bowles J,Koopman P,Lehnert S

更新日期：2008-10-14 00:00:00

abstract：BACKGROUND:Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which check...

journal_title：BMC molecular biology

authors： Mickle KL,Oliva A,Huberman JA,Leatherwood J

更新日期：2007-12-21 00:00:00

abstract：BACKGROUND:Modulation of chromatin structure has emerged as a critical molecular device to control gene expression. Histones undergo different post-translational modifications that increase chromatin accessibility to a number of regulatory factors. Among them, histone ubiquitination appears relevant in nuclear processe...

journal_title：BMC molecular biology

authors： Pinato S,Scandiuzzi C,Arnaudo N,Citterio E,Gaudino G,Penengo L

更新日期：2009-06-05 00:00:00

abstract：BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...

journal_title：BMC molecular biology

authors： Di Lisi R,Picard A,Ausoni S,Schiaffino S

更新日期：2007-09-17 00:00:00

abstract：BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...

journal_title：BMC molecular biology

authors： Zhang Y,Liu T,Yan P,Huang T,Dewille J

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...

journal_title：BMC molecular biology

authors： Rutledge RG,Stewart D

更新日期：2008-10-30 00:00:00

abstract：BACKGROUND:The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The present study aimed to identify reference genes as internal controls for gene expression studies by RT-qPC...

journal_title：BMC molecular biology

authors： Li QQ,Skinner J,Bennett JE

更新日期：2012-06-29 00:00:00

abstract：BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...

journal_title：BMC molecular biology

authors： Ozber N,Baris I,Tatlici G,Gur I,Kilinc S,Unal EB,Kavakli IH

更新日期：2010-09-14 00:00:00

abstract：BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...

journal_title：BMC molecular biology

authors： Frandsen RJ,Andersson JA,Kristensen MB,Giese H

更新日期：2008-08-01 00:00:00

abstract：BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

journal_title：BMC molecular biology

authors： Fleming VA,Geng C,Ladd AN,Lou H

更新日期：2012-12-10 00:00:00

abstract：BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...

journal_title：BMC molecular biology

authors： Zhang SC,MacDonald KA,Baguma-Nibasheka M,Geldenhuys L,Casson AG,Murphy PR

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:Small proline rich protein (SPRR) 2A is one of 14 SPRR genes that encodes for a skin cross-linking protein, which confers structural integrity to the cornified keratinocyte cell envelope. New evidence, however, shows that SPRR2A is also a critical stress and wound repair modulator: it enables a variety of ba...

journal_title：BMC molecular biology

authors： Mizuguchi Y,Specht S,Lunz JG 3rd,Isse K,Corbitt N,Takizawa T,Demetris AJ

更新日期：2012-06-25 00:00:00

abstract：BACKGROUND:Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. RESULTS:Insertion of a TEV protease cleavage site into Cdc23 allows i...

journal_title：BMC molecular biology

authors： Yang X,Gregan J,Lindner K,Young H,Kearsey SE

更新日期：2005-06-07 00:00:00

abstract：BACKGROUND:Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70% of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified using a proteome analysis, the venom gland transcriptome is currently lacking. RESULTS:We constructed a cDNA library from the v...

journal_title：BMC molecular biology

authors： Suntravat M,Uzcategui NL,Atphaisit C,Helmke TJ,Lucena SE,Sánchez EE,Acosta AR

更新日期：2016-03-05 00:00:00

abstract：BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...

journal_title：BMC molecular biology

authors： Yahyaoui W,Callejo M,Price GB,Zannis-Hadjopoulos M

更新日期：2007-04-12 00:00:00

abstract：BACKGROUND:Traumatic brain injury models are widely studied, especially through gene expression, either to further understand implied biological mechanisms or to assess the efficiency of potential therapies. A large number of biological pathways are affected in brain trauma models, whose elucidation might greatly benef...

journal_title：BMC molecular biology

authors： Rhinn H,Marchand-Leroux C,Croci N,Plotkine M,Scherman D,Escriou V

更新日期：2008-07-08 00:00:00

abstract：BACKGROUND:Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression of α smooth muscle actin (α-SMA) that belongs to the family of the six conserved acti...

journal_title：BMC molecular biology

authors： Veres-Székely A,Pap D,Sziksz E,Jávorszky E,Rokonay R,Lippai R,Tory K,Fekete A,Tulassay T,Szabó AJ,Vannay Á

更新日期：2017-04-27 00:00:00

abstract：BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prom...

journal_title：BMC molecular biology

authors： Kucknoor AS,Mundodi V,Alderete JF

更新日期：2005-03-04 00:00:00

abstract：BACKGROUND:Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as their expression is a...

journal_title：BMC molecular biology

authors： Teste MA,Duquenne M,François JM,Parrou JL

更新日期：2009-10-30 00:00:00

abstract：BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...

journal_title：BMC molecular biology

authors： He H,Cheng M,Yang X,Meng J,He A,Zheng X,Li Z,Guo P,Pan Z,Zhan X

更新日期：2009-10-25 00:00:00

abstract：BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...

journal_title：BMC molecular biology

authors： Liddiard K,Welch JS,Lozach J,Heinz S,Glass CK,Greaves DR

更新日期：2006-11-29 00:00:00

abstract：BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...

journal_title：BMC molecular biology

authors： Passmore M,Nataatmadja M,Fraser JF

更新日期：2009-07-23 00:00:00

abstract：BACKGROUND:The human Sex Hormone Binding Globulin (SHBG) gene, located at 17p13.1, comprises, at least, two different transcription units regulated by two different promoters. The first transcription unit begins with the exon 1 sequence and is responsible for the production of plasma SHBG by the hepatocytes, while the ...

journal_title：BMC molecular biology

authors： Pinós T,Barbosa-Desongles A,Hurtado A,Santamaria-Martínez A,de Torres I,Morote J,Reventós J,Munell F

更新日期：2009-06-17 00:00:00

abstract：BACKGROUND:The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two reg...

journal_title：BMC molecular biology

authors： Yousef AF,Brandl CJ,Mymryk JS

更新日期：2009-04-17 00:00:00