Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression.

abstract：BACKGROUND:Traumatic brain injury models are widely studied, especially through gene expression, either to further understand implied biological mechanisms or to assess the efficiency of potential therapies. A large number of biological pathways are affected in brain trauma models, whose elucidation might greatly benef...

journal_title：BMC molecular biology

authors： Rhinn H,Marchand-Leroux C,Croci N,Plotkine M,Scherman D,Escriou V

更新日期：2008-07-08 00:00:00

abstract：BACKGROUND:The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably the most abundant class of enhancers contains A/U-rich sequences. We have tested the influence of SD sequence length and the presence of enhancers on the efficiency o...

journal_title：BMC molecular biology

authors： Vimberg V,Tats A,Remm M,Tenson T

更新日期：2007-10-31 00:00:00

abstract：BACKGROUND:Cyclic AMP-dependent protein kinase (PKA) plays a central role in regulation of energy metabolism. Upon stimulation of testicular Sertoli cells by follicle stimulating hormone (FSH), glycolysis is activated to increase the production of nutrients for the germ cells, and a new regulatory subunit of cAMP-depen...

journal_title：BMC molecular biology

authors： Dahle MK,Taskén K,Taskén KA

更新日期：2002-06-21 00:00:00

abstract：BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...

journal_title：BMC molecular biology

authors： Yahyaoui W,Callejo M,Price GB,Zannis-Hadjopoulos M

更新日期：2007-04-12 00:00:00

abstract：BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

journal_title：BMC molecular biology

authors： Fleming VA,Geng C,Ladd AN,Lou H

更新日期：2012-12-10 00:00:00

abstract：BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...

journal_title：BMC molecular biology

authors： Jin H,Vacic V,Girke T,Lonardi S,Zhu JK

更新日期：2008-01-14 00:00:00

abstract：BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...

journal_title：BMC molecular biology

authors： Skern R,Frost P,Nilsen F

更新日期：2005-04-26 00:00:00

abstract：BACKGROUND:Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference ("housekeeping") gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping ...

journal_title：BMC molecular biology

authors： McCurley AT,Callard GV

更新日期：2008-11-12 00:00:00

abstract：BACKGROUND:Senescence marker protein-30 (SMP30), whose expression declines during aging in rat liver, has been proposed as an important aging marker. Besides apoptosis, SMP30 also protects cells against various other injuries by enhancement of membrane calcium-pump activity. The mechanism of this differential gene expr...

journal_title：BMC molecular biology

authors： Rath B,Pandey RS,Debata PR,Maruyama N,Supakar PC

更新日期：2008-04-29 00:00:00

abstract：BACKGROUND:The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or of limited use. RESU...

journal_title：BMC molecular biology

authors： Camacho Londoño J,Philipp SE

更新日期：2016-03-15 00:00:00

abstract：BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...

journal_title：BMC molecular biology

authors： He H,Cheng M,Yang X,Meng J,He A,Zheng X,Li Z,Guo P,Pan Z,Zhan X

更新日期：2009-10-25 00:00:00

abstract：BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...

journal_title：BMC molecular biology

authors： Thiel G,Al Sarraj J,Stefano L

更新日期：2005-01-19 00:00:00

abstract：BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...

journal_title：BMC molecular biology

authors： Ordinario EC,Yabuki M,Handa P,Cummings WJ,Maizels N

更新日期：2009-10-28 00:00:00

abstract：BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently f...

journal_title：BMC molecular biology

authors： Li Y,Podsypanina K,Liu X,Crane A,Tan LK,Parsons R,Varmus HE

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...

journal_title：BMC molecular biology

authors： Smith RJ,Savoian MS,Weber LE,Park JH

更新日期：2016-11-04 00:00:00

abstract：BACKGROUND:Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its e...

journal_title：BMC molecular biology

authors： Han F,Wang Z,Wu F,Liu Z,Huang B,Wang D

更新日期：2010-12-11 00:00:00

abstract：BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...

journal_title：BMC molecular biology

authors： Bondino HG,Valle EM

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays critical functions in many biological processes, including DNA repair and gene transcription. The main function of PARP-1 is to catalyze the transfer of ADP-ribose units from nicotinamide adenine dinucleotide (NAD+) to a large array of acc...

journal_title：BMC molecular biology

authors： Zaniolo K,Desnoyers S,Leclerc S,Guérin SL

更新日期：2007-10-25 00:00:00

abstract：BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...

journal_title：BMC molecular biology

authors： Liddiard K,Welch JS,Lozach J,Heinz S,Glass CK,Greaves DR

更新日期：2006-11-29 00:00:00

abstract：BACKGROUND:Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS:Rat RGC-5 cells were cultured in plates and H2O2 was added to induce o...

journal_title：BMC molecular biology

authors： Kong N,Lu X,Li B

更新日期：2014-11-18 00:00:00

abstract：BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...

journal_title：BMC molecular biology

authors： Rutledge RG,Stewart D

更新日期：2008-10-30 00:00:00

abstract：BACKGROUND:Naturally occurring tRNAs contain numerous modified nucleosides. They are formed by enzymatic modification of the primary transcripts during the complex RNA maturation process. In model organisms Escherichia coli and Saccharomyces cerevisiae most enzymes involved in this process have been identified. Interes...

journal_title：BMC molecular biology

authors： Purta E,van Vliet F,Tkaczuk KL,Dunin-Horkawicz S,Mori H,Droogmans L,Bujnicki JM

更新日期：2006-07-18 00:00:00

abstract：BACKGROUND:The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no information is available about Y RNAs in Chinese hamster cells, which have already been used to detect repli...

journal_title：BMC molecular biology

authors： de Lima Neto QA,Duarte Junior FF,Bueno PS,Seixas FA,Kowalski MP,Kheir E,Krude T,Fernandez MA

更新日期：2016-01-05 00:00:00

abstract：BACKGROUND:Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. RESULTS:Insertion of a TEV protease cleavage site into Cdc23 allows i...

journal_title：BMC molecular biology

authors： Yang X,Gregan J,Lindner K,Young H,Kearsey SE

更新日期：2005-06-07 00:00:00

abstract：BACKGROUND:Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which check...

journal_title：BMC molecular biology

authors： Mickle KL,Oliva A,Huberman JA,Leatherwood J

更新日期：2007-12-21 00:00:00

abstract：BACKGROUND:ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hype...

journal_title：BMC molecular biology

authors： Huang Q,Liu L,Liu J,Ni J,She Q,Shen Y

更新日期：2015-02-14 00:00:00

abstract：BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...

journal_title：BMC molecular biology

authors： Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo P

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal pathway. However, the role of RA and Stra8 in germ cell meiotic initiation in teleosts is poorly ch...

journal_title：BMC molecular biology

authors： Dong R,Yang S,Jiao J,Wang T,Shi H,Zhou L,Zhang Y,Wang D

更新日期：2013-05-22 00:00:00

abstract：BACKGROUND:Small nucleolar RNAs (snoRNAs) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs (rRNAs) and small nuclear RNAs (snRNAs). In recent years, more and more snoRNAs have been found to play novel roles in mRNA regulation, such as pre-mRNA splicing or RNA editing. In our previous study, ...

journal_title：BMC molecular biology

authors： Wu B,Huang L,Qiu W,Liu X,Shen Y,Lu Y,Yang Z,Li X,Cui B,Xu S,Qiao H,Qiu R,Yao L,Kan Y,Li D

更新日期：2019-04-11 00:00:00

abstract：BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...

journal_title：BMC molecular biology

authors： Yan B,Li D,Gou K

更新日期：2010-08-13 00:00:00