USF2 inhibits C/EBP-mediated transcriptional regulation of the RIIbeta subunit of cAMP-dependent protein kinase.

abstract：BACKGROUND:Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference ("housekeeping") gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping ...

journal_title：BMC molecular biology

authors： McCurley AT,Callard GV

更新日期：2008-11-12 00:00:00

abstract：BACKGROUND:Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its e...

journal_title：BMC molecular biology

authors： Han F,Wang Z,Wu F,Liu Z,Huang B,Wang D

更新日期：2010-12-11 00:00:00

abstract：BACKGROUND:The human Sex Hormone Binding Globulin (SHBG) gene, located at 17p13.1, comprises, at least, two different transcription units regulated by two different promoters. The first transcription unit begins with the exon 1 sequence and is responsible for the production of plasma SHBG by the hepatocytes, while the ...

journal_title：BMC molecular biology

authors： Pinós T,Barbosa-Desongles A,Hurtado A,Santamaria-Martínez A,de Torres I,Morote J,Reventós J,Munell F

更新日期：2009-06-17 00:00:00

abstract：BACKGROUND:Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. It is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins. The biochemical proper...

journal_title：BMC molecular biology

authors： Kim YG,Yoo JS,Kim JH,Kim CM,Oh JW

更新日期：2007-07-11 00:00:00

abstract：BACKGROUND:Visceral leishmaniasis caused by members of the Leishmania donovani complex is often fatal in the absence of treatment. Research has been hampered by the lack of good laboratory models and tools for genetic manipulation. In this study, we have characterised a L. infantum line (JPCM5) that was isolated from a...

journal_title：BMC molecular biology

authors： Denise H,Poot J,Jiménez M,Ambit A,Herrmann DC,Vermeulen AN,Coombs GH,Mottram JC

更新日期：2006-11-13 00:00:00

abstract：BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prom...

journal_title：BMC molecular biology

authors： Kucknoor AS,Mundodi V,Alderete JF

更新日期：2005-03-04 00:00:00

abstract：BACKGROUND:MicroRNAs (miRNAs) are short non-protein-coding RNA species that have a regulatory function in modulating protein translation and degradation of specific mRNAs. MicroRNAs are estimated to target approximately 60% of all human mRNAs and are associated with the regulation of all physiological processes. Simila...

journal_title：BMC molecular biology

authors： Tonge DP,Gant TW

更新日期：2016-02-09 00:00:00

abstract：BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...

journal_title：BMC molecular biology

authors： Kivistik PA,Kivi R,Kivisaar M,Hõrak R

更新日期：2009-05-16 00:00:00

abstract：BACKGROUND:Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. RESULTS:Insertion of a TEV protease cleavage site into Cdc23 allows i...

journal_title：BMC molecular biology

authors： Yang X,Gregan J,Lindner K,Young H,Kearsey SE

更新日期：2005-06-07 00:00:00

abstract：BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...

journal_title：BMC molecular biology

authors： Yan B,Li D,Gou K

更新日期：2010-08-13 00:00:00

abstract：BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...

journal_title：BMC molecular biology

authors： Mickle KL,Ramanathan S,Rosebrock A,Oliva A,Chaudari A,Yompakdee C,Scott D,Leatherwood J,Huberman JA

更新日期：2007-12-19 00:00:00

abstract：BACKGROUND:The L1 cell adhesion molecule (L1CAM) was originally identified as a neural adhesion molecule involved in axon guidance. In many human epithelial carcinomas L1CAM is overexpressed and thereby augments cell motility, invasion and metastasis formation. L1CAM positive carcinomas are associated with bad prognosi...

journal_title：BMC molecular biology

authors： Pfeifer M,Schirmer U,Geismann C,Schäfer H,Sebens S,Altevogt P

更新日期：2010-08-27 00:00:00

abstract：BACKGROUND:RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal pathway. However, the role of RA and Stra8 in germ cell meiotic initiation in teleosts is poorly ch...

journal_title：BMC molecular biology

authors： Dong R,Yang S,Jiao J,Wang T,Shi H,Zhou L,Zhang Y,Wang D

更新日期：2013-05-22 00:00:00

abstract：BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...

journal_title：BMC molecular biology

authors： O'Brochta DA,Stosic CD,Pilitt K,Subramanian RA,Hice RH,Atkinson PW

更新日期：2009-12-14 00:00:00

abstract：BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...

journal_title：BMC molecular biology

authors： Dedman AM,Majeed Y,Tumova S,Zeng F,Kumar B,Munsch C,Bateson AN,Wittmann J,Jäck HM,Porter KE,Beech DJ

更新日期：2011-07-12 00:00:00

abstract：BACKGROUND:The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The present study aimed to identify reference genes as internal controls for gene expression studies by RT-qPC...

journal_title：BMC molecular biology

authors： Li QQ,Skinner J,Bennett JE

更新日期：2012-06-29 00:00:00

abstract：BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...

journal_title：BMC molecular biology

authors： Ordinario EC,Yabuki M,Handa P,Cummings WJ,Maizels N

更新日期：2009-10-28 00:00:00

abstract：BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...

journal_title：BMC molecular biology

authors： He H,Cheng M,Yang X,Meng J,He A,Zheng X,Li Z,Guo P,Pan Z,Zhan X

更新日期：2009-10-25 00:00:00

abstract：BACKGROUND:Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression of α smooth muscle actin (α-SMA) that belongs to the family of the six conserved acti...

journal_title：BMC molecular biology

authors： Veres-Székely A,Pap D,Sziksz E,Jávorszky E,Rokonay R,Lippai R,Tory K,Fekete A,Tulassay T,Szabó AJ,Vannay Á

更新日期：2017-04-27 00:00:00

abstract：BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...

journal_title：BMC molecular biology

authors： Zhang Y,Liu T,Yan P,Huang T,Dewille J

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active platelet binding ability, and at the same time the rethrombosis complication after successful recana...

journal_title：BMC molecular biology

authors： Chen H,Mo W,Su H,Zhang Y,Song H

更新日期：2007-10-07 00:00:00

abstract：BACKGROUND:Physical exercise can improve brain function by altering brain gene expression. The expression mechanisms underlying the brain's response to exercise still remain unknown. miRNAs as vital regulators of gene expression may be involved in regulation of brain genes in response to exercise. However, as yet, very...

journal_title：BMC molecular biology

authors： Zhao Y,Zhang A,Wang Y,Hu S,Zhang R,Qian S

更新日期：2019-01-15 00:00:00

abstract：BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...

journal_title：BMC molecular biology

authors： Bondino HG,Valle EM

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:Previous studies suggest that human RAD9 (hRad9), encoding a DNA damage checkpoint molecule, which is frequently amplified in epithelial tumor cells of breast, lung, head and neck cancer, participates in regulation of the tumor suppressor p53-dependent transactivation of pro-survival P21WAF1. This study exam...

journal_title：BMC molecular biology

authors： Ishikawa K,Ishii H,Murakumo Y,Mimori K,Kobayashi M,Yamamoto K,Mori M,Nishino H,Furukawa Y,Ichimura K

更新日期：2007-05-21 00:00:00

abstract：BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...

journal_title：BMC molecular biology

authors： Skern R,Frost P,Nilsen F

更新日期：2005-04-26 00:00:00

abstract：BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...

journal_title：BMC molecular biology

authors： Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo P

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or of limited use. RESU...

journal_title：BMC molecular biology

authors： Camacho Londoño J,Philipp SE

更新日期：2016-03-15 00:00:00

abstract：BACKGROUND:Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regula...

journal_title：BMC molecular biology

authors： Calvo E,Grzenda A,Lomberk G,Mathison A,Iovanna J,Urrutia R

更新日期：2014-05-25 00:00:00

abstract：BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...

journal_title：BMC molecular biology

authors： Ozber N,Baris I,Tatlici G,Gur I,Kilinc S,Unal EB,Kavakli IH

更新日期：2010-09-14 00:00:00

abstract：BACKGROUND:Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purificatio...

journal_title：BMC molecular biology

authors： Voss C,Schmitt B,Werner-Simon S,Lutz C,Simon W,Anderl J

更新日期：2014-04-03 00:00:00