Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment.

abstract：BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prom...

journal_title：BMC molecular biology

authors： Kucknoor AS,Mundodi V,Alderete JF

更新日期：2005-03-04 00:00:00

abstract：BACKGROUND:Pseudo-nitzschia multiseries Hasle (Hasle) (Ps-n) is distinctive among the ecologically important marine diatoms because it produces the neurotoxin domoic acid. Although the biology of Ps-n has been investigated intensely, the characterization of the genes and biochemical pathways leading to domoic acid bios...

journal_title：BMC molecular biology

authors： Boissonneault KR,Henningsen BM,Bates SS,Robertson DL,Milton S,Pelletier J,Hogan DA,Housman DE

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...

journal_title：BMC molecular biology

authors： Jin H,Vacic V,Girke T,Lonardi S,Zhu JK

更新日期：2008-01-14 00:00:00

abstract：BACKGROUND:Although the complete genome sequences of a large number of organisms have been determined, the exact proteomes need to be characterized. More specifically, the extent to which post-translational processes such as proteolysis affect the synthesized proteins has remained unappreciated. We examined this issue ...

journal_title：BMC molecular biology

authors： Kumar R,Musiyenko A,Oldenburg A,Adams B,Barik S

更新日期：2004-07-01 00:00:00

abstract：BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...

journal_title：BMC molecular biology

authors： Kivistik PA,Kivi R,Kivisaar M,Hõrak R

更新日期：2009-05-16 00:00:00

abstract：BACKGROUND:The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no information is available about Y RNAs in Chinese hamster cells, which have already been used to detect repli...

journal_title：BMC molecular biology

authors： de Lima Neto QA,Duarte Junior FF,Bueno PS,Seixas FA,Kowalski MP,Kheir E,Krude T,Fernandez MA

更新日期：2016-01-05 00:00:00

abstract：BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

journal_title：BMC molecular biology

authors： Fleming VA,Geng C,Ladd AN,Lou H

更新日期：2012-12-10 00:00:00

abstract：BACKGROUND:Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active platelet binding ability, and at the same time the rethrombosis complication after successful recana...

journal_title：BMC molecular biology

authors： Chen H,Mo W,Su H,Zhang Y,Song H

更新日期：2007-10-07 00:00:00

abstract：BACKGROUND:Myocyte stress 1 (MS1) is a striated muscle actin binding protein required for the muscle specific activity of the evolutionary ancient myocardin related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathway. To date, little is known about the molecular mechanisms that govern skelet...

journal_title：BMC molecular biology

authors： Ounzain S,Dacwag CS,Samani NJ,Imbalzano AN,Chong NW

更新日期：2008-05-19 00:00:00

abstract：BACKGROUND:Double-strand breakage of chromosomal DNA is obviously a serious threat to cells because various activities of the chromosome depend on its integrity. However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, p...

journal_title：BMC molecular biology

authors： Handa N,Kobayashi I

更新日期：2003-04-28 00:00:00

abstract：BACKGROUND:RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal pathway. However, the role of RA and Stra8 in germ cell meiotic initiation in teleosts is poorly ch...

journal_title：BMC molecular biology

authors： Dong R,Yang S,Jiao J,Wang T,Shi H,Zhou L,Zhang Y,Wang D

更新日期：2013-05-22 00:00:00

abstract：BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...

journal_title：BMC molecular biology

authors： Yahyaoui W,Callejo M,Price GB,Zannis-Hadjopoulos M

更新日期：2007-04-12 00:00:00

abstract：BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...

journal_title：BMC molecular biology

authors： Di Lisi R,Picard A,Ausoni S,Schiaffino S

更新日期：2007-09-17 00:00:00

abstract：BACKGROUND:cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol described by Hubank and Schatz. RESULTS:In the post-hybridization mix, the 5'-ends of homoduplexes of inte...

journal_title：BMC molecular biology

authors： Kuvbachieva AA,Goffinet AM

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...

journal_title：BMC molecular biology

authors： Mickle KL,Ramanathan S,Rosebrock A,Oliva A,Chaudari A,Yompakdee C,Scott D,Leatherwood J,Huberman JA

更新日期：2007-12-19 00:00:00

abstract：BACKGROUND:ALL1-fused from chromosome 1q (AF1q), originally considered as an oncogenic factor, has been implicated in neuronal development; however, its upstream regulatory mechanisms in neural system remained elusive. RESULTS:Our study showed that REST (RE1 silencing transcription factor), a key transcription factor ...

journal_title：BMC molecular biology

authors： Hu Y,Sun Q,Zhang C,Sha Q,Sun X

更新日期：2015-09-05 00:00:00

abstract：BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...

journal_title：BMC molecular biology

authors： Smith RJ,Savoian MS,Weber LE,Park JH

更新日期：2016-11-04 00:00:00

abstract：BACKGROUND:Improving the treatment of renal cell carcinoma (RCC) will depend on the development of better biomarkers for predicting disease progression and aiding the design of appropriate therapies. One such marker may be fatty acid binding protein 7 (FABP7), also known as B-FABP and BLBP, which is expressed normally ...

journal_title：BMC molecular biology

authors： Takaoka N,Takayama T,Teratani T,Sugiyama T,Mugiya S,Ozono S

更新日期：2011-07-19 00:00:00

abstract：BACKGROUND:Physiological long term adaptation of pancreatic beta cells is driven by stimuli such as glucose and incretin hormones acting via cAMP (e.g. GLP-1) and involves regulated gene expression. Several rapidly inducible immediate-early genes (IEGs) have been identified in beta cells. Many of these IEGs code for tr...

journal_title：BMC molecular biology

authors： Glauser DA,Brun T,Gauthier BR,Schlegel W

更新日期：2007-06-22 00:00:00

abstract：BACKGROUND:ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hype...

journal_title：BMC molecular biology

authors： Huang Q,Liu L,Liu J,Ni J,She Q,Shen Y

更新日期：2015-02-14 00:00:00

abstract：BACKGROUND:Invitrogen Gateway technology exploits the integrase/att site-specific recombination system for directional cloning of PCR products and the subsequent subcloning into destination vectors. One or three DNA segments can be cloned using Gateway or MultiSite Gateway respectively. A vast number of single-site Gat...

journal_title：BMC molecular biology

authors： Magnani E,Bartling L,Hake S

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...

journal_title：BMC molecular biology

authors： Zhang Y,Liu T,Yan P,Huang T,Dewille J

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:SRY is the pivotal gene initiating male sex determination in most mammals, but how its expression is regulated is still not understood. In this study we derived novel SRY 5' flanking genomic sequence data from bovine and caprine genomic BAC clones. RESULTS:We identified four intervals of high homology upstr...

journal_title：BMC molecular biology

authors： Ross DG,Bowles J,Koopman P,Lehnert S

更新日期：2008-10-14 00:00:00

abstract：BACKGROUND:Single-stranded DNA-binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA. Human single-stranded DNA-binding protein 1 (hSSB1) is one such protein, with described roles in genome stability maintenance and transcriptional regulation. A...

journal_title：BMC molecular biology

authors： Ashton NW,Loo D,Paquet N,O'Byrne KJ,Richard DJ

更新日期：2016-12-09 00:00:00

abstract：BACKGROUND:Modulation of chromatin structure has emerged as a critical molecular device to control gene expression. Histones undergo different post-translational modifications that increase chromatin accessibility to a number of regulatory factors. Among them, histone ubiquitination appears relevant in nuclear processe...

journal_title：BMC molecular biology

authors： Pinato S,Scandiuzzi C,Arnaudo N,Citterio E,Gaudino G,Penengo L

更新日期：2009-06-05 00:00:00

abstract：BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...

journal_title：BMC molecular biology

authors： Gervais AL,Gaudreau L

更新日期：2009-03-04 00:00:00

abstract：BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...

journal_title：BMC molecular biology

authors： Thiel G,Al Sarraj J,Stefano L

更新日期：2005-01-19 00:00:00

abstract：BACKGROUND:Small nucleolar RNAs (snoRNAs) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs (rRNAs) and small nuclear RNAs (snRNAs). In recent years, more and more snoRNAs have been found to play novel roles in mRNA regulation, such as pre-mRNA splicing or RNA editing. In our previous study, ...

journal_title：BMC molecular biology

authors： Wu B,Huang L,Qiu W,Liu X,Shen Y,Lu Y,Yang Z,Li X,Cui B,Xu S,Qiao H,Qiu R,Yao L,Kan Y,Li D

更新日期：2019-04-11 00:00:00

abstract：BACKGROUND:The Y chromosome of mammals is particularly prone to accumulate genes related to male fertility. However, the high rate of molecular evolution on this chromosome predicts reduced power to the across-species comparative approach in identifying male-specific genes that are essential for sperm production in hum...

journal_title：BMC molecular biology

authors： Lopes AM,Miguel RN,Sargent CA,Ellis PJ,Amorim A,Affara NA

更新日期：2010-05-07 00:00:00

abstract：BACKGROUND:Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS:Rat RGC-5 cells were cultured in plates and H2O2 was added to induce o...

journal_title：BMC molecular biology

authors： Kong N,Lu X,Li B

更新日期：2014-11-18 00:00:00