Comparative in silico analysis identifies bona fide MyoD binding sites within the Myocyte stress 1 gene promoter.

abstract：BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...

journal_title：BMC molecular biology

authors： He H,Cheng M,Yang X,Meng J,He A,Zheng X,Li Z,Guo P,Pan Z,Zhan X

更新日期：2009-10-25 00:00:00

abstract：BACKGROUND:Visceral leishmaniasis caused by members of the Leishmania donovani complex is often fatal in the absence of treatment. Research has been hampered by the lack of good laboratory models and tools for genetic manipulation. In this study, we have characterised a L. infantum line (JPCM5) that was isolated from a...

journal_title：BMC molecular biology

authors： Denise H,Poot J,Jiménez M,Ambit A,Herrmann DC,Vermeulen AN,Coombs GH,Mottram JC

更新日期：2006-11-13 00:00:00

abstract：BACKGROUND:The L1 cell adhesion molecule (L1CAM) was originally identified as a neural adhesion molecule involved in axon guidance. In many human epithelial carcinomas L1CAM is overexpressed and thereby augments cell motility, invasion and metastasis formation. L1CAM positive carcinomas are associated with bad prognosi...

journal_title：BMC molecular biology

authors： Pfeifer M,Schirmer U,Geismann C,Schäfer H,Sebens S,Altevogt P

更新日期：2010-08-27 00:00:00

abstract：BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

journal_title：BMC molecular biology

authors： Fleming VA,Geng C,Ladd AN,Lou H

更新日期：2012-12-10 00:00:00

abstract：BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...

journal_title：BMC molecular biology

authors： Yan B,Li D,Gou K

更新日期：2010-08-13 00:00:00

abstract：BACKGROUND:Double-strand breakage of chromosomal DNA is obviously a serious threat to cells because various activities of the chromosome depend on its integrity. However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, p...

journal_title：BMC molecular biology

authors： Handa N,Kobayashi I

更新日期：2003-04-28 00:00:00

abstract：BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...

journal_title：BMC molecular biology

authors： Zhang Y,Liu T,Yan P,Huang T,Dewille J

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two reg...

journal_title：BMC molecular biology

authors： Yousef AF,Brandl CJ,Mymryk JS

更新日期：2009-04-17 00:00:00

abstract：BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently f...

journal_title：BMC molecular biology

authors： Li Y,Podsypanina K,Liu X,Crane A,Tan LK,Parsons R,Varmus HE

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The present study aimed to identify reference genes as internal controls for gene expression studies by RT-qPC...

journal_title：BMC molecular biology

authors： Li QQ,Skinner J,Bennett JE

更新日期：2012-06-29 00:00:00

abstract：BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...

journal_title：BMC molecular biology

authors： Bondino HG,Valle EM

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:Improving the treatment of renal cell carcinoma (RCC) will depend on the development of better biomarkers for predicting disease progression and aiding the design of appropriate therapies. One such marker may be fatty acid binding protein 7 (FABP7), also known as B-FABP and BLBP, which is expressed normally ...

journal_title：BMC molecular biology

authors： Takaoka N,Takayama T,Teratani T,Sugiyama T,Mugiya S,Ozono S

更新日期：2011-07-19 00:00:00

abstract：BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...

journal_title：BMC molecular biology

authors： Liddiard K,Welch JS,Lozach J,Heinz S,Glass CK,Greaves DR

更新日期：2006-11-29 00:00:00

abstract：BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...

journal_title：BMC molecular biology

authors： Dedman AM,Majeed Y,Tumova S,Zeng F,Kumar B,Munsch C,Bateson AN,Wittmann J,Jäck HM,Porter KE,Beech DJ

更新日期：2011-07-12 00:00:00

abstract：BACKGROUND:Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70% of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified using a proteome analysis, the venom gland transcriptome is currently lacking. RESULTS:We constructed a cDNA library from the v...

journal_title：BMC molecular biology

authors： Suntravat M,Uzcategui NL,Atphaisit C,Helmke TJ,Lucena SE,Sánchez EE,Acosta AR

更新日期：2016-03-05 00:00:00

abstract：BACKGROUND:ALL1-fused from chromosome 1q (AF1q), originally considered as an oncogenic factor, has been implicated in neuronal development; however, its upstream regulatory mechanisms in neural system remained elusive. RESULTS:Our study showed that REST (RE1 silencing transcription factor), a key transcription factor ...

journal_title：BMC molecular biology

authors： Hu Y,Sun Q,Zhang C,Sha Q,Sun X

更新日期：2015-09-05 00:00:00

abstract：BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...

journal_title：BMC molecular biology

authors： Yahyaoui W,Callejo M,Price GB,Zannis-Hadjopoulos M

更新日期：2007-04-12 00:00:00

abstract：BACKGROUND:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays critical functions in many biological processes, including DNA repair and gene transcription. The main function of PARP-1 is to catalyze the transfer of ADP-ribose units from nicotinamide adenine dinucleotide (NAD+) to a large array of acc...

journal_title：BMC molecular biology

authors： Zaniolo K,Desnoyers S,Leclerc S,Guérin SL

更新日期：2007-10-25 00:00:00

abstract：BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prom...

journal_title：BMC molecular biology

authors： Kucknoor AS,Mundodi V,Alderete JF

更新日期：2005-03-04 00:00:00

abstract：BACKGROUND:Previous studies suggest that human RAD9 (hRad9), encoding a DNA damage checkpoint molecule, which is frequently amplified in epithelial tumor cells of breast, lung, head and neck cancer, participates in regulation of the tumor suppressor p53-dependent transactivation of pro-survival P21WAF1. This study exam...

journal_title：BMC molecular biology

authors： Ishikawa K,Ishii H,Murakumo Y,Mimori K,Kobayashi M,Yamamoto K,Mori M,Nishino H,Furukawa Y,Ichimura K

更新日期：2007-05-21 00:00:00

abstract：BACKGROUND:Small proline rich protein (SPRR) 2A is one of 14 SPRR genes that encodes for a skin cross-linking protein, which confers structural integrity to the cornified keratinocyte cell envelope. New evidence, however, shows that SPRR2A is also a critical stress and wound repair modulator: it enables a variety of ba...

journal_title：BMC molecular biology

authors： Mizuguchi Y,Specht S,Lunz JG 3rd,Isse K,Corbitt N,Takizawa T,Demetris AJ

更新日期：2012-06-25 00:00:00

abstract：BACKGROUND:Duchenne Muscular Dystrophy (DMD) is an X-linked genetic disorder that results in the production of a dysfunctional form of the protein, dystrophin. The mdx5cv mouse is a model of DMD in which a point mutation in exon 10 of the dystrophin gene creates an artificial splice site. As a result, a 53 base pair de...

journal_title：BMC molecular biology

authors： Maguire K,Suzuki T,DiMatteo D,Parekh-Olmedo H,Kmiec E

更新日期：2009-02-23 00:00:00

abstract：BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...

journal_title：BMC molecular biology

authors： Di Lisi R,Picard A,Ausoni S,Schiaffino S

更新日期：2007-09-17 00:00:00

abstract：BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...

journal_title：BMC molecular biology

authors： Skern R,Frost P,Nilsen F

更新日期：2005-04-26 00:00:00

abstract：BACKGROUND:Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regula...

journal_title：BMC molecular biology

authors： Calvo E,Grzenda A,Lomberk G,Mathison A,Iovanna J,Urrutia R

更新日期：2014-05-25 00:00:00

abstract：BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...

journal_title：BMC molecular biology

authors： Smith RJ,Savoian MS,Weber LE,Park JH

更新日期：2016-11-04 00:00:00

abstract：BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...

journal_title：BMC molecular biology

authors： Thiel G,Al Sarraj J,Stefano L

更新日期：2005-01-19 00:00:00

abstract：BACKGROUND:Single-stranded DNA-binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA. Human single-stranded DNA-binding protein 1 (hSSB1) is one such protein, with described roles in genome stability maintenance and transcriptional regulation. A...

journal_title：BMC molecular biology

authors： Ashton NW,Loo D,Paquet N,O'Byrne KJ,Richard DJ

更新日期：2016-12-09 00:00:00

abstract：BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...

journal_title：BMC molecular biology

authors： Passmore M,Nataatmadja M,Fraser JF

更新日期：2009-07-23 00:00:00

abstract：BACKGROUND:Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that...

journal_title：BMC molecular biology

authors： Garcia AC,Gitaí DL,Humann FC,Paçó-Larson ML,Monesi N

更新日期：2011-08-01 00:00:00