Abstract：
BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently found, especially in mice under the age of six months. RESULTS:To better understand the role of PTEN in breast tumor development, we have crossed Pten heterozygous mice to MMTV-Wnt-1 transgenic mice that routinely develop ductal carcinomas in the mammary gland. Female Wnt-1 transgenics heterozygous for Pten developed mammary tumors earlier than Wnt-1 transgenics that were wild type for Pten. In most tumors arising in Pten heterozygotes, the Pten wild-type allele was lost, suggesting that cells lacking Pten function have a growth advantage over cells retaining a wild type allele. Tumors with LOH contained high levels of activated AKT/PKB, a downstream target of the PTEN/PI3K pathway. CONCLUSIONS:An animal model has been developed in which the absence of Pten collaborates with Wnt-1 to induce ductal carcinoma in the mammary gland. This animal model may be useful for testing therapies specific for tumors deregulated in the PTEN/PI3K/AKT pathway.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Li Y,Podsypanina K,Liu X,Crane A,Tan LK,Parsons R,Varmus HEdoi
10.1186/1471-2199-2-2keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
2issn
1471-2199journal_volume
2pub_type
杂志文章abstract：BACKGROUND:cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol described by Hubank and Schatz. RESULTS:In the post-hybridization mix, the 5'-ends of homoduplexes of inte...
journal_title：BMC molecular biology
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更新日期：2012-06-29 00:00:00
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更新日期：2016-12-09 00:00:00
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更新日期：2007-06-22 00:00:00
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journal_title：BMC molecular biology
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journal_title：BMC molecular biology
pub_type： 杂志文章
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更新日期：2003-04-28 00:00:00
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journal_title：BMC molecular biology
pub_type： 杂志文章
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更新日期：2008-01-14 00:00:00
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journal_title：BMC molecular biology
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更新日期：2008-10-14 00:00:00
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journal_title：BMC molecular biology
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更新日期：2009-03-04 00:00:00
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journal_title：BMC molecular biology
pub_type： 杂志文章
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更新日期：2007-04-12 00:00:00
