Abstract:
BACKGROUND:Small nucleolar RNAs (snoRNAs) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs (rRNAs) and small nuclear RNAs (snRNAs). In recent years, more and more snoRNAs have been found to play novel roles in mRNA regulation, such as pre-mRNA splicing or RNA editing. In our previous study, we found a silkworm C/D box snoRNA Bm-15 can interact with Notch receptor gene in vitro. To further study the function of Bm-15, we cloned its homolog Sf-15 from Spodoptera frugiperda and investigate the function of Sf-15 in Sf9 cells. RESULTS:We showed that knocking down of Sf-15 can inhibit the proliferation, then induce apoptosis of insect S. frugiperda Sf9 cells, but the results were reversed when Sf-15 was overexpressed. De novo sequencing of transcriptome of Sf9 cells showed that the expression of 21 apoptosis-related genes were increased upon Sf-15 repression. Further analysis showed that a Ca2+-induced cell death pathway gene Cn (PPP3C, the serine/threonine-protein phosphatase 2B catalytic subunit), was significantly increased upon Sf-15 depression but decreased when Sf-15 was overexpressed, which indicated that Cn might be a potential target of Sf-15. CONCLUSIONS:We conclude that C/D box snoRNA Sf-15 can participate in apoptosis through regulating the expression of Ca2+-induced cell death pathway gene Cn in Sf9 cells. This is the first time that we found snoRNAs exhibiting dual functions in insect, which reveals a novel layer of ncRNA modulation in cell growth and death.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Wu B,Huang L,Qiu W,Liu X,Shen Y,Lu Y,Yang Z,Li X,Cui B,Xu S,Qiao H,Qiu R,Yao L,Kan Y,Li Ddoi
10.1186/s12867-019-0128-9subject
Has Abstractpub_date
2019-04-11 00:00:00pages
12issue
1issn
1471-2199pii
10.1186/s12867-019-0128-9journal_volume
20pub_type
杂志文章abstract:BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...
journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2008-10-30 00:00:00
abstract:BACKGROUND:ALL1-fused from chromosome 1q (AF1q), originally considered as an oncogenic factor, has been implicated in neuronal development; however, its upstream regulatory mechanisms in neural system remained elusive. RESULTS:Our study showed that REST (RE1 silencing transcription factor), a key transcription factor ...
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pub_type: 杂志文章
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更新日期:2015-09-05 00:00:00
abstract:BACKGROUND:Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression of α smooth muscle actin (α-SMA) that belongs to the family of the six conserved acti...
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pub_type: 杂志文章
doi:10.1186/1471-2199-10-98
更新日期:2009-10-28 00:00:00
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更新日期:2009-10-25 00:00:00
abstract:BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...
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journal_title:BMC molecular biology
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更新日期:2009-10-16 00:00:00
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更新日期:2012-06-29 00:00:00
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更新日期:2009-10-30 00:00:00
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更新日期:2008-11-12 00:00:00
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更新日期:2011-07-12 00:00:00
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更新日期:2009-03-04 00:00:00
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更新日期:2005-03-04 00:00:00
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更新日期:2009-04-17 00:00:00
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更新日期:2007-06-22 00:00:00
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更新日期:2018-07-27 00:00:00
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更新日期:2016-03-15 00:00:00
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