Abstract:
BACKGROUND:Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its exact function remains unclear. RESULTS:Sox30 cDNA was cloned from the Nile tilapia by RT-PCR and RACE. Screening of available genome and EST databases and phylogenetic analysis showed that Sox30 also exists in non-mammalian vertebrates and invertebrates, which was further supported by synteny analyses. Tissue expression in human, mouse and tilapia suggested that Sox30 was probably a gonad-specific gene, which was also supported by the fact that Sox30 EST sequences were obtained from gonads of the animal species. In addition, four alternatively spliced isoforms were isolated from tilapia gonad. Their temporal and spatial expression patterns during normal and sex reversed gonadal development were investigated by RT-PCR and in situ hybridization. Our data suggest that expressions of Sox30 isoforms are related to stage and phenotypic-sex, observed in the germ cells of male gonad and in somatic cells of the female gonad. CONCLUSIONS:Sox30 is not a gene only existed in mammals, but exists widely throughout the animal kingdom as supported by our bioinformatic, phylogenetic and syntenic analyses. It is very likely that Sox30 is expressed exclusively in gonads. Expression analyses revealed that Sox30 may be involved in female and male gonadal development at different stages by alternative splicing.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Han F,Wang Z,Wu F,Liu Z,Huang B,Wang Ddoi
10.1186/1471-2199-11-98subject
Has Abstractpub_date
2010-12-11 00:00:00pages
98issn
1471-2199pii
1471-2199-11-98journal_volume
11pub_type
杂志文章abstract:BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-83
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...
journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2008-10-30 00:00:00
abstract:BACKGROUND:Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purificatio...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-15-7
更新日期:2014-04-03 00:00:00
abstract:BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-11
更新日期:2008-01-23 00:00:00
abstract:BACKGROUND:Although the complete genome sequences of a large number of organisms have been determined, the exact proteomes need to be characterized. More specifically, the extent to which post-translational processes such as proteolysis affect the synthesized proteins has remained unappreciated. We examined this issue ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-5-6
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...
journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-6-10
更新日期:2005-04-26 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2008-07-08 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2016-01-05 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2018-07-27 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-43
更新日期:2008-04-29 00:00:00
abstract:BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-27
更新日期:2007-04-12 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2006-11-13 00:00:00
abstract:BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-6
更新日期:2008-01-14 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-3-10
更新日期:2002-06-21 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-3-6
更新日期:2002-04-24 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0075-7
更新日期:2016-11-04 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0030-z
更新日期:2015-02-14 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0077-5
更新日期:2016-12-09 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2012-06-25 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2006-12-06 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-6-5
更新日期:2005-03-04 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-15
更新日期:2009-02-23 00:00:00
abstract:BACKGROUND:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays critical functions in many biological processes, including DNA repair and gene transcription. The main function of PARP-1 is to catalyze the transfer of ADP-ribose units from nicotinamide adenine dinucleotide (NAD+) to a large array of acc...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-96
更新日期:2007-10-25 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2003-04-28 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-5-14
更新日期:2004-08-26 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-7-23
更新日期:2006-07-18 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2009-10-16 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2007-12-21 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
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更新日期:2009-04-17 00:00:00