Dephosphorylation of specific proteins in HL-60 cells by 1 alpha, 25-dihydroxyvitamin D3: possible involvement of cAMP-dependent protein kinase.


:Changes of phosphoprotein patterns in HL-60 cells were studied during short exposures to 1 alpha, 25-dihydroxyvitamin D3 [1,25(OH)2D3]. One hundred nanometers 1,25(OH)2D3 dephosphorylated at least three proteins in 6 h: phosphoproteins with molecular weights of 82 kD (pp82), 33 kD (pp33), and 31 kD (pp31). Phosphorylation of pp33 and pp31 was also suppressed by 1 mM dbcAMP, and dephosphorylation of the two protein by 1,25(OH)2D3 was inhibited by 8 microM H-8, an inhibitor of cAMP-dependent protein kinase (PKA). Furthermore, 8 microM H-8 inhibited dephosphorylation of the two proteins when it was added with 1,25(OH)2D3. On the other hand, 10 nM TPA gave no significant change to these two phosphoproteins. These results suggest the possibility that PKA is involved in the early stages of 1,25(OH)2D3-induced HL-60 cell differentiation through specific protein dephosphorylation.


Leuk Res


Leukemia research


Ueda T,Sawada H,Okuda T,Yumoto Y,Kato Y,Ogawa K,Tashima M,Yoshida Y,Okuma M





Has Abstract


1991-01-01 00:00:00














  • Bortezomib in combination with dexamethasone and subsequent thalidomide for newly-diagnosed multiple myeloma: a Chinese experience.

    abstract:OBJECTIVE:Bortezomib-dexamethasone-thalidomide has been reported to be effective in newly-diagnosed multiple myeloma (MM) with an overall response rate of 92% and a CR rate of 18% (Alexanian et al, Hematology 2007;12(3):235-9), but this regimen has not been tested in the Chinese patients. We report here our results tes...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Zheng W,Wei G,Ye X,He J,Li L,Wu W,Shi J,Zhang J,Huang W,Xie W,Luo Y,Xue X,Lin M,Huang H,Cai Z

    更新日期:2009-12-01 00:00:00

  • Mesenchymal stem cells derived from multiple myeloma patients protect against chemotherapy through autophagy-dependent activation of NF-κB signaling.

    abstract::Chemotherapy resistance has been considered as a major problem for multiple myeloma (MM) treatment and bone marrow microenvironment plays a crucial role in the MM progression and chemoresistance. Recent studies reported that bone marrow mesenchymal stem cells derived from MM patients (MM-MSCs) revealed various charact...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Yang H,Zheng Y,Zhang Y,Cao Z,Jiang Y

    更新日期:2017-09-01 00:00:00

  • Indomethacin induces apoptosis and inhibits proliferation in chronic myeloid leukemia cells.

    abstract::A nonsteroidal anti-inflammatory drug (NSAID)--indomethacin (IN), was found to induce apoptosis and inhibit proliferation of K562 cells and primary culture bone marrow cells from six chronic myelogenous leukemia (CML) patients. IN induced cells apoptosis and inhibited cells proliferation in a dose-and time-dependent m...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Zhang G,Tu C,Zhang G,Zhou G,Zheng W

    更新日期:2000-05-01 00:00:00

  • Simultaneous inhibition of DNA methyltransferase and histone deacetylase induces p53-independent apoptosis via down-regulation of Mcl-1 in acute myelogenous leukemia cells.

    abstract::We have recently established the MV4-11 acute myelogenous leukemia (AML) subline, designated as MV4-11 TP53 R248W, which possesses a missense mutation (CGG→TGG; R248W) in the TP53 gene, leading to inactivation of this transcription factor. DNA methyltransferase (DNMT) inhibitor 5-Aza-2'-deoxycytidine (5-AzadC) induced...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Nishioka C,Ikezoe T,Yang J,Udaka K,Yokoyama A

    更新日期:2011-07-01 00:00:00

  • Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma.

    abstract::Mantle cell lymphoma (MCL) is a distinct type of B cell malignancy and accounts for approximately 5-10% of non-Hodgkin's lymphomas (NHL). The characteristic cytogenetic aberration in MCL is the translocation (11;14)(q13;q32) present in virtually all cases. This rearrangement at the BCL1 locus at 11q13 dysregulates the...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Drexler HG,MacLeod RA

    更新日期:2002-09-01 00:00:00

  • Synergism between clofarabine and decitabine through p53R2: a pharmacodynamic drug-drug interaction modeling.

    abstract::Clofarabine (CLO), a purine nucleoside analog with promising efficacy in acute myeloid leukemia (AML), inhibits the ribonucleotidereductase, p53R2. We have shown that p53R2 mRNA is up-regulated by decitabine (DEC), another drug with promising activity in AML. We developed a pharmacodynamic model to characterize the in...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Thudium KE,Ghoshal S,Fetterly GJ,Haese JP,Karpf AR,Wetzler M

    更新日期:2012-11-01 00:00:00

  • Characterization of cell markers in type B retroviral-induced thymic lymphomas--I. Surface antigen phenotype and karyotype in developing and primary lymphomas.

    abstract::CFW/D mice injected neonatally with a type B retrovirus, (DMBA-LV), rapidly develop thymic lymphomas. The present study examined simultaneously the karyotype and the expression of surface antigens on thymocytes from DMBA-LV treated mice at 28, 35 and 42 days of age during the development of lymphomas, and of cells fro...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Mueller RE,Ball JK,Chan FP

    更新日期:1989-01-01 00:00:00

  • WITHDRAWN: The effect of dihydroartemisinin on TfR and VEGF expression in iron overload human myeloid leukemia K562 cells.

    abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Zengwang,Zhou HJ,Li A,Zhang JL

    更新日期:2009-02-20 00:00:00

  • Fragment analysis represents a suitable approach for the detection of hotspot c.7541_7542delCT NOTCH1 mutation in chronic lymphocytic leukemia.

    abstract::The hotspot c.7541_7542delCT NOTCH1 mutation has been proven to have a negative clinical impact in chronic lymphocytic leukemia (CLL). However, an optimal method for its detection has not yet been specified. The aim of our study was to examine the presence of the NOTCH1 mutation in CLL using three commonly used molecu...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Vavrova E,Kantorova B,Vonkova B,Kabathova J,Skuhrova-Francova H,Diviskova E,Letocha O,Kotaskova J,Brychtova Y,Doubek M,Mayer J,Pospisilova S

    更新日期:2017-09-01 00:00:00

  • Effects of X-irradiation, ovariohysterectomy and estradiol-17 beta on incidence, benign/malignant ratio and multiplicity of rat mammary neoplasms--a preliminary report.

    abstract::An overview is given of the effects of X-irradiation, ovariohysterectomy and estradiol-17 beta administration on mammary tumorigenesis in females of 3 rat strains, viz. the WAG/Rij, BN/BiRij and SD. The 3 rat strains differed significantly in their spontaneous mammary tumor incidence. Female SD rats had the highest in...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Solleveld HA,van Zwieten MJ,Broerse JJ,Hollander CF

    更新日期:1986-01-01 00:00:00

  • Prognostic significance of FLT3 mutations in pediatric non-promyelocytic acute myeloid leukemia.

    abstract::FLT3 is a receptor tyrosine kinase involved in the survival of hematopoietic stem cells, and mutations of FLT3 have been reported to be of prognostic significance. This is the first study of FLT3 mutations in pediatric non-promyelocytic AML patients that received the same treatment scheme in single institute. FLT3 int...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kang HJ,Hong SH,Kim IH,Park BK,Han KS,Cho HI,Shin HY,Ahn HS

    更新日期:2005-06-01 00:00:00

  • Relation between chelation and clinical outcomes in lower-risk patients with myelodysplastic syndromes: Registry analysis at 5 years.

    abstract::Prospective data are needed to ascertain the impact of iron chelation therapy in patients with myelodysplastic syndromes. The present 5-year prospective registry analysis was conducted to compare clinical outcomes between chelated and nonchelated patients with lower-risk myelodysplastic syndromes and transfusional iro...

    journal_title:Leukemia research

    pub_type: 杂志文章,多中心研究


    authors: Lyons RM,Marek BJ,Paley C,Esposito J,McNamara K,Richards PD,DiBella N,Garcia-Manero G

    更新日期:2017-05-01 00:00:00

  • Interleukin 4 production by peripheral blood lymphocytes in patients with classical Hodgkin lymphoma.

    abstract::Although the production of interleukin (IL) 2 and interferon (IFN) gamma by peripheral blood lymphocytes in patients with Hodgkin lymphoma (HL) is well documented, the synthesis of IL4 has not been investigated before. The present study examines the production of IL4 by 2-day phytohaemaglutinin (PHA)-stimulated periph...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Mainou-Fowler T,Proctor SJ,Taylor PR

    更新日期:2004-02-01 00:00:00

  • Transfer of the cytidine deaminase cDNA into hematopoietic cells.

    abstract::In order to investigate whether transfer of the cytidine deaminase (CDD) cDNA would increase chemotherapy resistance to cytosine arabinoside (ara-C) we used a retroviral vector expressing both, neomycin phosphotransferase and the CDD cDNA, to transduce hematopoietic cells from cell lines and from murine bone marrow (B...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Flasshove M,Frings W,Schröder JK,Moritz T,Schütte J,Seeber S

    更新日期:1999-11-01 00:00:00

  • Single-dose mitoxantrone in combination with continuous infusion intermediate-dose cytarabine plus etoposide for treatment of refractory or early relapsed acute myeloid leukemia.

    abstract::This prospective phase II clinical trial evaluated the effects of single-dose mitoxantrone (36 mg/m2 on day 1) in combination with continuous infusion intermediate-dose cytarabine plus etoposide in 25 patients with refractory or early relapsed acute myeloid leukemia (AML). We compared the results of our current study ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Lee SS,Lee JH,Lee JH,Kim DY,Kim SH,Lim SN,Lee YS,Seol M,Ryu SG,Kang YA,Jang S,Park CJ,Chi HS,Yun SC,Lee KH

    更新日期:2009-04-01 00:00:00

  • Serum double monoclonal components and hematological malignancies: only a casual association? Review of 34 cases.

    abstract::A double monoclonal component (MC) detected in the serum and/or urine represents a very rare occurrence (2-6% of monoclonal gammopathies). In this study, we report 34 patients with double serum MCs, focusing on the associated diseases. The diagnosis was made using high-resolution serum protein electrophoresis and immu...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Guastafierro S,Ferrara MG,Sica A,Parascandola RR,Santangelo S,Falcone U

    更新日期:2012-10-01 00:00:00

  • Acute myeloid leukemia with hypergranular cytoplasm accompanied by t(X;11)(q24;q23) and rearrangement of the MLL gene.

    abstract::This report describes a unique case of acute myeloid leukemia with hypergranular cytoplasm and t(X;11)(q24;q23). The breakpoint on 11q23 was identified within the MLL gene. The hypergranular cytoplasm of leukemic cells and the associated coagulopathy resembled a characteristic of acute promyelocytic leukemia, despite ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Nakata Y,Mori T,Yamazaki T,Suzuki T,Okazaki T,Kurosawa Y,Kinoshita A,Ohyashiki K,Nakazawa S

    更新日期:1999-01-01 00:00:00

  • Smoking as a contributing factor for development of polycythemia vera and related neoplasms.

    abstract::Smoking may be associated with accelerated erythropoiesis, leukocytosis and thrombocytosis, which are also hallmarks in patients with polycythemia vera, essential thrombocythemia and early stages of myelofibrosis (MPNs). The JAK-STAT and NF-κB signaling pathways are activated in both smokers and in patients with MPNs....

    journal_title:Leukemia research

    pub_type: 杂志文章,评审


    authors: Hasselbalch HC

    更新日期:2015-09-09 00:00:00

  • MTHFR 677CC/1298CC genotypes are highly associated with chronic myelogenous leukemia: a case-control study in Korea.

    abstract::Methylenetetrahydrofolate reductase (MTHFR) is an enzyme involved in folate metabolism and DNA methylation. Studies on MTHFR polymorphism in leukemia have largely focused on the protective role of MTHFR polymorphism in acute lymphoblastic leukemia (ALL). We evaluated the C677T and A1298C polymorphisms using the TaqMan...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Moon HW,Kim TY,Oh BR,Min HC,Cho HI,Bang SM,Lee JH,Yoon SS,Lee DS

    更新日期:2007-09-01 00:00:00

  • Inherited pericentric inversion of chromosome 16 in chronic phase of chronic myeloid leukaemia.

    abstract::The simultaneous occurrence of two specific acquired chromosomal abnormalities in chronic or acute leukaemias is rare. Inherited chromosomal abnormalities are also rare events in the general population. In chronic myeloid leukaemia (CML), characterised by the t(9;22)(q34;q11), the inv(16)(p13q22) has been described as...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Silva PM,Lourenço GJ,Bognone RA,Delamain MT,Pinto-Junior W,Lima CS

    更新日期:2006-01-01 00:00:00

  • The reproducibility of acute lymphoblastic leukemia phenotype determinations: evaluation of monoclonal antibody and conventional hematopoietic markers.

    abstract::Peripheral blood and/or bone marrow lymphoblasts from 25 patients with acute lymphoblastic leukemia (ALL) were tested with a panel of monoclonal antibodies (MoAbs) and conventional hematopoietic markers by three different laboratories. The results were analysed to evaluate the reproducibility of ALL phenotype determin...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Sobol RE,Mick R,LeBien TW,Ozer H,Minowada J,Anderson K,Ellison RR,Cuttner J,Morrison A,Richards F 2nd

    更新日期:1986-01-01 00:00:00

  • Four additional cases of trisomy 14 as the sole anomaly in various haematological malignancies.

    abstract::We report on four cases of trisomy 14 as the sole anomaly. Three cases were myelodysplastic syndromes and one was a non-Hodgkin's lymphoma. This anomaly is mainly in myeloid disorders and still remains to be well documented. On the other hand, we show this anomaly to be also a non-random anomaly in lymphoproliferative...

    journal_title:Leukemia research

    pub_type: 杂志文章,评审


    authors: Brizard A,Guilhot F,Babin P,Burucoa C,Tanzer J,Huret JL

    更新日期:1992-01-01 00:00:00

  • Long-term culture of infant leukemia cells: dependence upon stromal cells from the bone marrow and bilineage differentiation.

    abstract::Infant leukemia cells with 46XY,t(11; 17)(q23; p13) karyotype and a hybrid pre B myeloid phenotype (HLA-DR, (Ia), B4 and My7-positive and CALLA and T11-negative) and immunoglobulin heavy chain gene rearrangement were maintained in long-term culture for over 10 months. The in-vitro survival and growth of the leukemia c...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Umiel T,Friedman S,Zaizov R,Cohen IJ,Gozes Y,Epstein N,Kobiler D,Zipori D

    更新日期:1986-01-01 00:00:00

  • Genotypic and clinical heterogeneity within NCCN favorable-risk acute myeloid leukemia.

    abstract::The National Comprehensive Cancer Network (NCCN) defines the following types of acute myeloid leukemia (AML) as favorable-risk: acute promyelocytic leukemia with t(15;17) (APL); AML with core-binding factor (CBF) rearrangements, including t(8;21) and inv(16) or t(16;16) without mutations in KIT (CBF-KITwt); and AML wi...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Strickland SA,Shaver AC,Byrne M,Daber RD,Ferrell PB,Head DR,Mohan SR,Mosse CA,Moyo TK,Stricker TP,Vnencak-Jones C,Savona MR,Seegmiller AC

    更新日期:2018-02-01 00:00:00

  • Characterization of acute myeloid leukemia with del(9q) - Impact of the genes in the minimally deleted region.

    abstract::Acute myeloid leukemia is an aggressive disease that arises from clonal expansion of malignant hematopoietic precursor cells of the bone marrow. Deletions on the long arm of chromosome 9 (del(9q)) are observed in 2% of acute myeloid leukemia patients. Our deletion analysis in a cohort of 31 del(9q) acute myeloid leuke...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Naarmann-de Vries IS,Sackmann Y,Klein F,Ostareck-Lederer A,Ostareck DH,Jost E,Ehninger G,Brümmendorf TH,Marx G,Röllig C,Thiede C,Crysandt M

    更新日期:2019-01-01 00:00:00

  • Correlation between genetic polymorphisms of the hOCT1 and MDR1 genes and the response to imatinib in patients newly diagnosed with chronic-phase chronic myeloid leukemia.

    abstract::The association between seven polymorphisms in the genes hOCT1 and MDR1, encoding for imatinib transporter proteins, and the response to imatinib 400mg/daily was investigated in 65 patients newly diagnosed with chronic-phase chronic myeloid leukemia. The AA genotype at the rs6935207 hOCT1 polymorphic locus was not det...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Maffioli M,Camós M,Gaya A,Hernández-Boluda JC,Alvarez-Larrán A,Domingo A,Granell M,Guillem V,Vallansot R,Costa D,Bellosillo B,Colomer D,Cervantes F

    更新日期:2011-08-01 00:00:00

  • Low lymphocyte-to-monocyte ratio predicts unfavorable prognosis in non-germinal center type diffuse large B-cell lymphoma.

    abstract::The peripheral blood lymphocyte to monocyte ratio (LMR) at diagnosis has been used to predict survival in diffuse large B-cell lymphoma (DLBCL) patients, but its prognostic significance with respect to different cell-of-origin (COO) subtypes remains unknown. We retrospectively analyzed 168 de novo DLBCL patients in th...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Wei X,Huang F,Wei Y,Jing H,Xie M,Hao X,Feng R

    更新日期:2014-06-01 00:00:00

  • Decreased chronic lymphocytic leukemia incidence in Asians in Los Angeles County.

    abstract::Chronic lymphocytic leukemia (CLL) is rare in Asians living in Asia and possibly in US Asians. In contrast, CLL is the most common leukemia in whites. The basis for this ethnic and geographic variation is unknown. We compared average annual age-adjusted incidence rates (AAIR) of CLL diagnosed from 1972 to 1995 among L...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Gale RP,Cozen W,Goodman MT,Wang FF,Bernstein L

    更新日期:2000-08-01 00:00:00

  • Risk and outcomes of second malignant neoplasms in chronic myeloid leukemia survivors.

    abstract::The risk of second malignant neoplasms (SMN) in chronic myeloid leukemia (CML) survivors remains unclear. We utilized the Surveillance, Epidemiology and End Results 18 (SEER 18) registries to evaluate the risk and subsequent outcomes of SMN in CML survivors. There were 3407 patients included. Of these, 170 (4.99%) dev...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Jamy O,Sarmad R,Costa L

    更新日期:2019-07-01 00:00:00

  • A monoclonal antibody reactive with normal and leukemic human myeloid progenitor cells.

    abstract::Anti-MY9 is an IgG2b murine monoclonal antibody selected for reactivity with immature normal human myeloid cells. The MY9 antigen is expressed by blasts, promyelocytes and myelocytes in the bone marrow, and by monocytes in the peripheral blood. Erythrocytes, lymphocytes and platelets are MY9 negative. All myeloid colo...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Griffin JD,Linch D,Sabbath K,Larcom P,Schlossman SF

    更新日期:1984-01-01 00:00:00