Abstract:
BACKGROUND:For most cases of idiopathic acquired sideroblastic anemia (IASA), the molecular pathogenesis is unknown, despite the consistent morphological signature of abundant pathological ringed sideroblasts with their characteristic iron-engorged mitochondria. Moderately elevated free erythrocyte protoporphyrin (FEP) levels have been described in IASA, suggesting that the activity of ferrochelatase, the enzyme that catalyzes the final step in heme biosynthesis (incorporation of ferrous iron into protoporphyrin), might be diminished in erythroid progenitor cells from IASA patients. METHODS:We confirmed FEP elevation in IASA, then pursued a candidate gene approach that included screening the gene encoding ferrochelatase, FECH, for promoter and coding region mutations and mRNA expression changes in bone marrow from 37 patients with IASA. RESULTS:The analytical techniques employed detected mutations in a test cohort of previously undiagnosed patients with biochemical evidence for erythropoietic protoporphyria, a condition resulting from germline mutations in FECH, but somatic missense mutations of FECH and its promoter were not observed in IASA patients. FECH was modestly overexpressed in progenitor cells from patients with IASA, compared with MDS patients without sideroblasts and healthy controls. In addition, we analyzed ABCB7 and PUS1, genes implicated in congenital sideroblastic anemia syndromes, but again found no coding mutations in acquired cases. CONCLUSION:We conclude that acquired mutations in the factors currently known to cause inherited sideroblastic anemias are uncommon in IASA.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Steensma DP,Hecksel KA,Porcher JC,Lasho TLdoi
10.1016/j.leukres.2006.06.005subject
Has Abstractpub_date
2007-05-01 00:00:00pages
623-8issue
5eissn
0145-2126issn
1873-5835pii
S0145-2126(06)00219-0journal_volume
31pub_type
杂志文章abstract::Regulation of pluripotent stem cell (CFU-S) proliferation kinetics by humoral factors is now well documented. However, the mechanism of choice of CFU-S differentiation pathways is still a controversial issue. We suggest that long-range humoral factors (pluripoietins) are capable of preferentially channelling CFU-S tow...
journal_title:Leukemia research
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journal_title:Leukemia research
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journal_title:Leukemia research
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pub_type: 杂志文章,多中心研究
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journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
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journal_title:Leukemia research
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journal_title:Leukemia research
pub_type: 杂志文章
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journal_title:Leukemia research
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.06.019
更新日期:2008-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.08.022
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,多中心研究
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journal_title:Leukemia research
pub_type: 杂志文章
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.11.010
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journal_title:Leukemia research
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doi:10.1016/j.leukres.2003.10.016
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journal_title:Leukemia research
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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