Abstract:
:We examined the effect of deferasirox (DFX) on CD34+ hematopoietic progenitors from MDS patients. Progressive, dose-dependent suppression of MDS progenitor proliferation in culture was observed with DFX concentrations ranging from 5 μM to 20 μM. This effect was more pronounced in MDS compared to CD34+ progenitors isolated from umbilical cord blood or normal peripheral blood. There was reduced viability of MDS progenitors but not normal progenitors at 20 μM DFX which increased with duration of exposure. Exposure to 20 μM DFX for 14 days markedly suppressed colony growth of MDS progenitors. Reactive oxygen species levels were elevated above control at concentrations of DFX above 5 μM. We conclude that exposure to DFX results in dose-dependent inhibition of proliferation, and survival in MDS progenitors.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Pullarkat V,Sehgal A,Li L,Meng Z,Lin A,Forman S,Bhatia Rdoi
10.1016/j.leukres.2012.03.018subject
Has Abstractpub_date
2012-08-01 00:00:00pages
966-73issue
8eissn
0145-2126issn
1873-5835pii
S0145-2126(12)00159-2journal_volume
36pub_type
杂志文章abstract::Imatinib mesylate, a Bcr/Abl tyrosine kinase inhibitor, is widely used in treating chronic myeloid leukemia. However, drug-resistance of leukemia cells becomes an emergent problem. Herein, various flavonoids were screened for applicability in leukemia treatment, and 3-hydroxyflavone (3-HF) was found to be most effecti...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.05.018
更新日期:2012-09-01 00:00:00
abstract::The study was undertaken to elucidate the regulatory roles of GMP reductase (GMPR) and IMP dehydrogenase (IMPDH) on purine interconversion during differentiation. Treatment of HL-60 cells with retinoic acid (1 microM) induced granulocytic differentiation which was accompanied with a 2.4-fold increase in GMPR and 55% d...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(92)90002-o
更新日期:1992-06-01 00:00:00
abstract::The culture supernatants from 43 human cell lines obtained during log phase and from purified normal peripheral blood B-lymphocytes cultured at 10(6) cells ml-1 for 48 h in RPMI 1640-5% fetal calf serum were examined for interleukin-8 (IL-8) using Elisa kits. Constitutive IL-8 production was found for 14/15 B-cell lin...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(93)90164-g
更新日期:1993-12-01 00:00:00
abstract::This study focuses on the effect of chemotherapy on endocrinopathies and the metabolic syndrome in adult survivors of childhood acute myeloid leukemia (AML). Endocrine function and metabolic syndrome were evaluated in 12 AML survivors, treated with chemotherapy, and in 9 survivors of myeloid leukemias treated with ste...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.12.008
更新日期:2013-04-01 00:00:00
abstract::Leukemic cells from 85 children with newly diagnosed precursor B-lineage ALL were tested for in vitro drug sensitivity to a panel of anti-cancer drugs. Minimal residual disease (MRD) was measured by RQ-PCR. There was a significant correlation between MRD day 29 and in vitro sensitivity to prednisolone (p<0.001) and do...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.06.012
更新日期:2009-01-01 00:00:00
abstract::We evaluated the efficacy of FISH to detect chromosome anomalies in the evaluation of young (<60 years) patients with AML. Patients were enrolled in E1900, an ECOG clinical trial for AML. The protocol was designed to collect bone marrow or blood for both cytogenetic and FISH studies at study entry (diagnosis). FISH fo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.07.026
更新日期:2007-05-01 00:00:00
abstract::Increasing evidence suggest that epigenetic mechanisms (e.g. histone modification by histone deacetylases) play a major role in the pathogenesis of Hodgkin's lymphoma (HL). We treated HL cell lines with the histone deacetylase inhibitor vorinostat and investigated the gene expression profile of these cells by using DN...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.02.021
更新日期:2012-06-01 00:00:00
abstract::Although abnormalities in granulopoiesis detected by means of bone marrow cytology, culture and kinetic studies have provided prognostic data in preleukemic states and oligoblastic leukemias, this information cannot be applied to individual cases. In order to determine the indications for treatment and the form it sho...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(83)90018-8
更新日期:1983-01-01 00:00:00
abstract::When spleen cells of mice grafted with STIL-3 C5 cells, a leukemic T-cell line producing IL-3, are cultured in vitro, a high IL-3 activity is detectable in the culture supernatant. However, when bone marrow cells of the same grafted mice are cultured under similar conditions, hardly any IL-3 activity is detectable. To...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(92)90035-6
更新日期:1992-09-01 00:00:00
abstract::Forty-three patients were studied to determine whether light chain gene rearrangements may occur in hematopoietic cells not pertaining to the B-lineage. In only one patient, affected by T-cell lymphoblastic lymphoma, one kappa light chain allele was rearranged. Neither at the protein level nor at the RNA level the rea...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90145-j
更新日期:1991-01-01 00:00:00
abstract::Acute myeloid leukemia (AML) is frequently treated with induction and consolidation chemotherapy. Consolidation chemotherapy can be delivered on an ambulatory basis, requiring some patients to travel long distances for treatment at specialized centers. We developed a shared care model where patients receive consolidat...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2017.05.023
更新日期:2017-08-01 00:00:00
abstract::Proto-oncogene transcriptional activation was analyzed in a group of MCF 247 MuLv-induced T-cell lymphomas to identify transformation-specific gene activations and determine whether the proviral insertion near a myc gene could promote a peculiar mechanism of transformation through a differential proto-oncogene express...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(90)90007-v
更新日期:1990-01-01 00:00:00
abstract::To further understand the role of XIAP in acute myeloid leukemia (AML), we suppressed XIAP expression by antisense oligonucleotides and determined the effect on gene expression profiles and biological pathways. XIAP inhibition upregulated expression of proteasome genes in a manner similar to the proteasome inhibitor b...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.04.018
更新日期:2013-08-01 00:00:00
abstract::Brazil is a wide country with huge contrasts. Its peculiarities can highlight environmental factors that could influence the frequencies of different cancers. The standard treatment and results achieved from several different areas of the country may not be found in others. The establishment of a national cooperative ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00187-4
更新日期:2002-07-01 00:00:00
abstract::The glycosylation of beta-hexosaminidase was investigated in the transformed cell-line, CCRF/CEM, derived from a human acute lymphoblastic leukaemia, and comparisons were made with enzyme from normal human skin fibroblasts. A series of studies including neuraminidase sensitivity, lectin chromatography, Biogel P4 chrom...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(88)90146-4
更新日期:1988-01-01 00:00:00
abstract::Occupational and industrial exposures have been implicated in the etiology of leukemia, yet uncertainty remains regarding potential high risk occupations. We examined the associations between self-reported occupations and hobbies and acute leukemia risk using data from 811 cases and 637 controls participating in a cas...
journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2005.03.002
更新日期:2005-10-01 00:00:00
abstract::Acute myeloid leukemia (AML) is a heterogeneous disease. Until recently, treatment for patients with AML was limited to induction chemotherapy with cytarabine and anthracycline or hypomethylating agents, and, in some instances, allogeneic hematopoietic stem cell transplant. With the recent approval of new therapies-i....
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2018.09.001
更新日期:2018-10-01 00:00:00
abstract::We studied 98 female patients in remission (2-240 months) from childhood ALL to determine the clonality status of their hematopoiesis. Thirty-one (31.6%) were heterozygous at the PGK locus for the BstX1 endonuclease restriction site, permitting X-linked clonality assays to be performed. Two patients were in relapse at...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90121-x
更新日期:1994-02-01 00:00:00
abstract::The genes for the CDK4/6-inhibitors p16INK4A/MTS1 and p15INK4B/MTS2 are frequently deleted in hematological malignancies. A new member of this family of CDK4/6 inhibitors is p18. In order to assess p18 growth-suppressor gene alterations in hematological neoplasms, we investigated 31 lymphoma and leukemia cell lines by...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00137-9
更新日期:1996-02-01 00:00:00
abstract::In 141 adult patients with diagnosis of acute myeloid leukemia the overall expression and intensity of expression of CD34 antigen on leukemic cells was investigated. Myeloid blasts were tested by applying direct immunofluorescence staining using anti-CD34 fluorescein monoclonal antibody in flow cytometry. CD34 antigen...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(97)00015-5
更新日期:1997-07-01 00:00:00
abstract::We have previously reported that serum stem factor (SCF) levels are significantly lower in patients with myelodysplastic syndrome (MDS) than normal controls. We have now studied the effects of adding SCF to cultures of blood mononuclear cells from patients with MDS and normal subjects. Three of 17 patients with MDS sh...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00034-l
更新日期:1995-08-01 00:00:00
abstract::A combination of monosomy 7 and translocation t(9;22) (q34;q11), rarely observed in acute lymphoblastic leukaemia (ALL), is here reported: a peculiarity of this case was that the "breakpoint cluster region" on chromosome 22 was not rearranged, as demonstrated by molecular analysis, and a new c-abl protein (p190) was f...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90114-7
更新日期:1987-01-01 00:00:00
abstract::Our previous studies have demonstrated that a previously unrecognized role of CFTR in hematopoiesis and acute leukemia. Here, we show that CFTR inhibitor CFTR-inh172 possesses ability to inhibit human T-cell acute lymphoblastic leukemia cells. In detail, CFTR-inh172 inhibited cell proliferation, promoted apoptosis and...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2019.106225
更新日期:2019-11-01 00:00:00
abstract::We examined the antineoplastic effects of the iron chelators, deferasirox and deferoxamine in multiple myeloma cell lines as well as primary myeloma cells. These iron chelators showed marked antiproliferative activity as well as cytotoxicity toward myeloma cell lines and deferasirox was cytotoxic to bone marrow plasma...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.06.005
更新日期:2014-08-01 00:00:00
abstract::Ectopic expression of LMO2 occurs in approximately 45% of T-lineage acute lymphoblastic leukemias (T-ALL), sometimes in association with chromosomal translocations. Recently, a lymphoproliferative disorder developed in two participants in a gene therapy trial due to LMO2 activation via integration of the retroviral ve...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.05.013
更新日期:2005-01-01 00:00:00
abstract::Although recent advances in chemotherapy have markedly improved outcome of acute lymphoblastic leukemia (ALL), infantile ALL with MLL gene rearrangements (MLL+ALL) is refractory to chemotherapy. We have shown that specific cytokines FLT3 ligand and TGFβ1 both of which are produced from bone marrow stromal cells synerg...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2018.11.001
更新日期:2018-12-01 00:00:00
abstract::It is generally accepted that morphologically recognizable bone marrow (BM) cells are derived from progenitor cells committed to a specific line of haematopoietic differentiation [51]. The origin and morphological identity of such progenitor cells is not yet known and the question, whether there is a single pluripoten...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/0145-2126(85)90130-4
更新日期:1985-01-01 00:00:00
abstract::Promoter hypermethylation occurs early in leukemogenesis and seems to be associated with poor prognosis in acute lymphoblastic leukemia (ALL). The methylation status of the promoter region of six genes was analyzed in 71 children with ALL using methylation specific PCR (MSP). Calcitonin (CALCA) and E-cadherin (CDH1) w...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.11.016
更新日期:2006-07-01 00:00:00
abstract::Although the earliest sites of hematopoietic cell and endothelial cell differentiation in the yolk sac blood islands were identified about 100 years ago, cells with hemangioblast properties have not yet been identified in vivo. Endothelial cells differentiate from angioblasts in the embryo and from endothelial progeni...
journal_title:Leukemia research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.leukres.2006.10.014
更新日期:2007-04-01 00:00:00
abstract::With the application of adequately sensitive tests, it is now becoming evident that more than 90% of patients with conventionally-defined polycythemia vera (PV) carry the somatic JAK2V617F mutation in their granulocytes. However, the specific mutation is also found in other classic and atypical myeloproliferative diso...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.01.004
更新日期:2006-06-01 00:00:00