Analysis of the novel cyclin-dependent kinase 4 and 6 inhibitor gene p18 in lymphoma and leukemia cell lines.


:The genes for the CDK4/6-inhibitors p16INK4A/MTS1 and p15INK4B/MTS2 are frequently deleted in hematological malignancies. A new member of this family of CDK4/6 inhibitors is p18. In order to assess p18 growth-suppressor gene alterations in hematological neoplasms, we investigated 31 lymphoma and leukemia cell lines by PCR for both exons of this gene. No homozygous deletions were observed. Investigation of a new intragenic restriction fragment length polymorphism revealed no differences in allele distribution between the tumor cell lines and healthy volunteers. Our results suggest that homozygous deletion of the p18 gene does not play a major role in leukemogenesis or lymphomagenesis.


Leuk Res


Leukemia research


Siebert R,Willers CP,Fosså A,Kloke O,Nowrousian MR,Seeber S,Opalka B




Has Abstract


1996-02-01 00:00:00














  • miR-155 regulative network in FLT3 mutated acute myeloid leukemia.

    abstract:BACKGROUND:Acute myeloid leukemia (AML) represents a heterogeneous disorder with recurrent chromosomal alterations and molecular abnormalities. Among AML with normal karyotype (NK-AML) FLT3 activating mutation, internal tandem duplication (FLT3-ITD), is present in about 30% of patients, conferring unfavorable outcome. ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Salemi D,Cammarata G,Agueli C,Augugliaro L,Corrado C,Bica MG,Raimondo S,Marfia A,Randazzo V,Dragotto P,Di Raimondo F,Alessandro R,Fabbiano F,Santoro A

    更新日期:2015-08-01 00:00:00

  • miR-125a regulates cell cycle, proliferation, and apoptosis by targeting the ErbB pathway in acute myeloid leukemia.

    abstract::microRNA profiling of acute myeloid leukemia patient samples identified miR-125a as being decreased. Current literature has investigated miR-125a's role in normal hematopoiesis but not within acute myeloid leukemia. Analysis of the upstream region of miR-125a identified several CpG islands. Both precursor and mature m...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Ufkin ML,Peterson S,Yang X,Driscoll H,Duarte C,Sathyanarayana P

    更新日期:2014-03-01 00:00:00

  • miRNA mediated up-regulation of cochaperone p23 acts as an anti-apoptotic factor in childhood acute lymphoblastic leukemia.

    abstract::p23 is a heat shock protein 90 (Hsp90) cochaperone that plays a significant role in estrogen receptor (ER) alpha signal transduction and telomerase activity; it is up-regulated in several cancers. Recent studies have found that high level of p23 may promote tumor progression and poor prognosis in breast cancer patient...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Liu X,Zou L,Zhu L,Zhang H,Du C,Li Z,Gao C,Zhao X,Bao S,Zheng H

    更新日期:2012-09-01 00:00:00

  • Differentiation induction in non-lymphocytic leukemia cells upon treatment with mycophenolate mofetil.

    abstract::Inosine 5'-monophosphate (IMP) dehydrogenase catalyzes the rate-limiting reaction of guanine nucleotide biosynthesis and has been implicated in the reaction of cell growth and differentiation. We investigated the ability of mycophenolate mofetil, a prodrug of mycophenolic acid, to induce differentiation in HL-60 and U...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Inai K,Tsutani H,Yamauchi T,Fukushima T,Iwasaki H,Imamura S,Wano Y,Nemoto Y,Naiki H,Ueda T

    更新日期:2000-09-01 00:00:00

  • Day 22 of induction therapy is important for minimal residual disease assessment by flow cytometry in childhood acute lymphoblastic leukemia.

    abstract::This study was aimed to illustrate the significance of minimal residual disease (MRD) assessment on day 22 in childhood acute lymphoblastic leukemia. MRD were measured on day 22, day 36, week 12, month 6 and month 12 by four-color flow cytometry. The 5-year cumulative incidence of relapse was significantly different f...

    journal_title:Leukemia research

    pub_type: 临床试验,杂志文章


    authors: Xu XJ,Tang YM,Shen HQ,Song H,Yang SL,Shi SW,Xu WQ

    更新日期:2012-08-01 00:00:00

  • Differential effect of heat shock on RNA metabolism in human Burkitt's lymphoma B-cell lines.

    abstract::Thermal stress induces expression of a family of heat shock proteins which may regulate the synthesis of various cellular genes. We investigated the effect of heat shock on polyadenylation in Epstein-Barr Virus (EBV) negative and EBV transformed human Burkitt's lymphoma (BL) B-cell lines. Incubation of the BL B-cell l...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kumar A,Kurl RN,Kryworuchko M,Diaz-Mitoma F,Sharma S

    更新日期:1995-11-01 00:00:00

  • Occupation, hobbies, and acute leukemia in adults.

    abstract::Occupational and industrial exposures have been implicated in the etiology of leukemia, yet uncertainty remains regarding potential high risk occupations. We examined the associations between self-reported occupations and hobbies and acute leukemia risk using data from 811 cases and 637 controls participating in a cas...

    journal_title:Leukemia research

    pub_type: 杂志文章,多中心研究


    authors: Terry PD,Shore DL,Rauscher GH,Sandler DP

    更新日期:2005-10-01 00:00:00

  • Combination therapy with arsenic trioxide, all-trans retinoic acid, and chemotherapy in acute promyelocytic leukemia patients with various relapse risks.

    abstract::To improve the recovery rate of high-risk patients with acute promyelocytic leukemia (APL), we used all-trans retinoic acid (ATRA)/arsenic trioxide (ATO)/daunorubicin combination in remission induction, daunorubicin and cytarabine in consolidation, and ATRA/ATO/methotrexate ± 6-mercaptopurine in maintenance treatment ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Huang H,Qin Y,Xu R,You X,Teng R,Yang L,Xu M,Liu H

    更新日期:2012-07-01 00:00:00

  • Use of the receptor tyrosine kinase-like orphan receptor 1 (ROR1) as a diagnostic tool in chronic lymphocytic leukemia (CLL).

    abstract::Flow cytometry is commonly used to establish the diagnosis of chronic lymphocytic leukemia (CLL). A defined combination of antibodies discriminates between normal B cells and CLL cells (coexpression of CD5, CD19, and CD23). The receptor tyrosine-like orphan receptor one (ROR1) is an embryonic glycoprotein involved in ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Uhrmacher S,Schmidt C,Erdfelder F,Poll-Wolbeck SJ,Gehrke I,Hallek M,Kreuzer KA

    更新日期:2011-10-01 00:00:00

  • Treatment of unfavorable myelodysplastic syndrome with all-trans-retinoic acid and subcutaneous Ara C.

    abstract::Thirty patients with high-risk myelodysplastic syndrome, defined as > 5% bone marrow blasts, were treated with a combination of oral all-trans-retinoic acid (45 mg/m2 daily) and subcutaneous AraC (10 mg/m2) on days 1-14 of each 28-35 day cycle repeated for 2-6 cycles. Complete remission lasting 9, 12, and 15 months wa...

    journal_title:Leukemia research

    pub_type: 临床试验,杂志文章


    authors: Letendre L,Noel P,White WL,Tefferi A,Litzow MR,Li CY,Schroeder G

    更新日期:1999-09-01 00:00:00

  • Differentiation restriction in the neutrophil-granulocyte, macrophage, eosinophil-granulocyte pathway: analysis by equilibrium density centrifugation.

    abstract::Bone marrow culture techniques and equilibrium density centrifugation of human bone marrow cells were used to analyse the neutrophil-granulocyte, macrophage and eosinophil-granulocyte progenitor hierarchy. The buoyant density of progenitor cells changes as cells differentiate down the granulocyte-macrophage pathway an...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Guimaraes JE,Francis GE,Bol SJ,Berney JJ,Hoffbrand AV

    更新日期:1982-01-01 00:00:00

  • Production of vascular endothelial growth factor in T-cell prolymphocytic leukemia.

    abstract::We describe a 79-year-old man who had massive pleural effusion and a proliferation of prolymphocytic leukemia cells in the peripheral blood, bone marrow, and pleural effusion fluid. Immunophenotyping of leukemia cells revealed either CD3+CD4+CD8-CD25+ or CD3+CD4+CD8+CD25+. The antibody against human T-cell lymphotropi...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Ogasawara T,Narita C,Kawauchi K

    更新日期:2007-03-01 00:00:00

  • Fragment analysis represents a suitable approach for the detection of hotspot c.7541_7542delCT NOTCH1 mutation in chronic lymphocytic leukemia.

    abstract::The hotspot c.7541_7542delCT NOTCH1 mutation has been proven to have a negative clinical impact in chronic lymphocytic leukemia (CLL). However, an optimal method for its detection has not yet been specified. The aim of our study was to examine the presence of the NOTCH1 mutation in CLL using three commonly used molecu...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Vavrova E,Kantorova B,Vonkova B,Kabathova J,Skuhrova-Francova H,Diviskova E,Letocha O,Kotaskova J,Brychtova Y,Doubek M,Mayer J,Pospisilova S

    更新日期:2017-09-01 00:00:00

  • The expression of PRAME in chronic lymphoproliferative disorders.

    abstract::The PRAME gene encodes an antigen recognized by autologous T lymphocytes and is expressed in trophoblasts, testis and frequently in human solid cancers and acute leukemias, making it a candidate for immunotherapy and for detecting MRD. We demonstrate expression of PRAME by RT-PCR in the peripheral blood or bone marrow...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Proto-Siqueira R,Falcão RP,de Souza CA,Ismael SJ,Zago MA

    更新日期:2003-05-01 00:00:00

  • Targeting the IL-17/IL-6 axis can alter growth of Chronic Lymphocytic Leukemia in vivo/in vitro.

    abstract::The tumor microenvironment (TME) is critical to the longevity of tumor B cells in chronic lymphocytic leukemia (CLL). Bone marrow mesenchymal stem cells (BMMSCs) and the cytokines they produce including IL-6 are important components of the TME in CLL. We found BMMSCs supported the survival of CLL cells in vitro throug...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Zhu F,McCaw L,Spaner DE,Gorczynski RM

    更新日期:2018-03-01 00:00:00

  • Enhanced topoisomerase II-induced DNA breaks and free radical production by a new anthracycline with potent antileukemic activity.

    abstract::In a previous study we reported that a new anthracycline derivative (moflomycin) exhibited a higher antileukemic activity compared to other anthracyclines, such as daunorubicin and doxorubicin. To explain the superior antileukemic effect of moflomycin and to disclose a possible structure-activity relationship, we inve...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Andrivon W,Saucier JM,Auclair C,Monneret C,Florent JC,Guillosson JJ,Nafziger J

    更新日期:1996-02-01 00:00:00

  • Leucocyte superoxide dismutase levels in acute and chronic leukemias.

    abstract::Superoxide dismutase "SOD" enzyme levels in leucocytes of patients with acute and chronic leukemia, were measured by using Misra and Fridovich's epinephrine method. In all types of leukemia, the SOD levels were found to be significantly higher than normals. However, these levels decreased to normal in remission. No re...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kökoğlu E,Aktuğlu G,Belce A

    更新日期:1989-01-01 00:00:00

  • Susceptibility of acute myeloid leukemia (AML) cells from clinically resistant and sensitive patients to daunomycin (DNR): assessment in vitro after stimulation with colony stimulating factors (CSFs).

    abstract::Chemosensitivity of purified AML blasts to daunomycin was examined under conditions of CSF stimulation and compared with clinical outcome of DNR combination chemotherapy. AML blasts from 16 patients were purified, incubated serum-free in the presence of optimal concentrations of a complete cocktail of IL-3, GM-CSF and...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Santini V,Nooter K,Delwel R,Löwenberg B

    更新日期:1990-01-01 00:00:00

  • Influence of rhGM-CSF on Ara-C sensitivity of patients with acute myeloid leukemia in relapse: a flow cytometry study.

    abstract::Twenty-three patients with acute myelogenous leukemia (AML) in first relapse were treated with high-dose cytosine-arabinoside (Ara-C) and amsacrine or idarubicin. To prime the cells, the patients were given rhGM-CSF. We studied the influence of 48-h infusion of rhGM-CSF on proliferation and Ara-C sensitivity of leukem...

    journal_title:Leukemia research

    pub_type: 临床试验,杂志文章


    authors: Lacombe F,Puntous M,Dumain P,Cony-Makhoul P,Belloc F,Bernard P,Boisseau M,Reiffers J

    更新日期:1996-06-01 00:00:00

  • Mitoxantrone and prolonged infusion gemcitabine as salvage therapy in patients with acute myelogenous leukemia.

    abstract::In a recent phase I study, a combination of gemcitabine at 10 mg/(m(2)min) for 12 h and mitoxantrone 12 mg/m(2) daily for 3 days, achieved a complete remission (CR) in 3 of 12 (25%) patients with refractory leukemia. A pilot assessment of this regimen was conducted to determine its tolerability in patients with refrac...

    journal_title:Leukemia research

    pub_type: 临床试验,杂志文章


    authors: Apostolidou E,Estey E,Cortes J,Garcia-Manero G,Faderl S,Thomas D,Tsimberidou A,Kantarjian H,Giles FJ

    更新日期:2003-04-01 00:00:00

  • Partial restoration of all trans retinoic acid (ATRA) sensitivity by compactin in ATRA-resistant leukemic cells (ATRA-R HL-60).

    abstract::The resistance to all trans retinoic acid (ATRA) differentiating treatment is a consequence, in most of the cases, of either increased catabolism or down regulation of ATRA uptake. Recently, we have shown that ATRA efficiency to differentiate HL-60 cells was enhanced about 30 times after its incorporation into Low Den...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Gueddari N,Madoulet C,Lefebvre P,Berthou L,Chomienne C,Jardillier JC

    更新日期:2000-06-01 00:00:00

  • Prognostic significance of FLT3 mutations in pediatric non-promyelocytic acute myeloid leukemia.

    abstract::FLT3 is a receptor tyrosine kinase involved in the survival of hematopoietic stem cells, and mutations of FLT3 have been reported to be of prognostic significance. This is the first study of FLT3 mutations in pediatric non-promyelocytic AML patients that received the same treatment scheme in single institute. FLT3 int...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kang HJ,Hong SH,Kim IH,Park BK,Han KS,Cho HI,Shin HY,Ahn HS

    更新日期:2005-06-01 00:00:00

  • Bone biopsy derived marrow stromal elements rescue chronic lymphocytic leukemia B-cells from spontaneous and drug induced cell death and facilitates an "angiogenic switch".

    abstract::A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kay NE,Shanafelt TD,Strege AK,Lee YK,Bone ND,Raza A

    更新日期:2007-07-01 00:00:00

  • Impact of gene mutations and chromosomal aberrations on progression-free survival in chronic lymphocytic leukemia patients treated with front-line chemoimmunotherapy: Clinical practice experience.

    abstract::The impact of genetic aberrations on rituximab-based therapeutic regimens has been intensely studied in chronic lymphocytic leukemia (CLL). According to the current consensus chemoimmunotherapy consisting of rituximab and DNA-damaging drugs is not suitable for patients with TP53 defects. In our study, we focused on CL...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Spunarova M,Tom N,Pavlova S,Mraz M,Brychtova Y,Doubek M,Panovska A,Skuhrova Francova H,Brzobohata A,Pospisilova S,Mayer J,Trbusek M

    更新日期:2019-06-01 00:00:00

  • Erythropoietin activates telomerase through transcriptional and posttranscriptional regulation in human erythroleukemic JAS-REN-A cells.

    abstract::We evaluated the molecular mechanism of telomerase activation by erythropoietin (EPO) in human erythroleukemic JAS-REN-A cells. Telomerase activity increased 3-4 fold after 3-24h of culture with EPO and was associated with increases in c-myc mRNA after 1-3h, of c-Myc protein after 3-6h, and of human telomerase reverse...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Akiyama M,Kawano T,Mikami-Terao Y,Agawa-Ohta M,Yamada O,Ida H,Yamada H

    更新日期:2011-03-01 00:00:00

  • Inhibition of sonic hedgehog signaling blocks cell migration and growth but induces apoptosis via suppression of FOXQ1 in natural killer/T-cell lymphoma.

    abstract::The present study explored the effects of Forkhead box Q1 (FOXQ1) on cell proliferation, cell cycle and apoptosis via the Sonic hedgehog (Shh) pathway in Natural killer/T-cell lymphoma (NKTCL). Quantitative real time-polymerase chain reaction (qRT-PCR) was performed to detect FOXQ1 expression in 117 NKTCL patients and...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Liu P,Chen L

    更新日期:2018-01-01 00:00:00

  • Long-term bone marrow culture-derived stromal fibroblasts as a potential target for gene therapy in acute myelogenous leukemia.

    abstract::As a part of our continuing efforts to develop gene therapy for acute myelogenous leukemia (AML), this study was undertaken to evaluate the possibility of using autologous bone marrow stromal fibroblasts (BMSFs) as a target cell population. Autologous BMSFs in AML were isolated from the stromal layers of long-term bon...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Min YH,Li GX,Jang JH,Suh HC,Kim JS,Cheong JW,Lee ST,Hahn JS,Ko YW

    更新日期:2002-04-01 00:00:00

  • Minimal residual disease in vitro of a pre-B-lymphoma.

    abstract::We have studied the survival of clonogenic neoplastic cells of a murine pre-B-lymphoma (BCl-1) of the spleen in culture. We have found quantitative deficiencies such as reduced surface adherence of stromal cells and impaired CFU-F (colony forming units-fibroblast) and pre-CFU-F colony and layer formation in stromal cu...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Prindull G,Ben-Ishay Z,Bergholz M,Prindull B

    更新日期:1993-07-01 00:00:00

  • Ectodomain shedding of CD200 from the B-CLL cell surface is regulated by ADAM28 expression.

    abstract::CD200, a membrane glycoprotein of the immunoglobulin superfamily, is overexpressed in CLL. Soluble in serum CD200 (sCD200) is correlated with poor prognosis in CLL. ADAM (a disintegrin and metalloproteinase) enzymes are implicated in membrane protein shedding. ADAM28 mRNA expression in CLL was correlated with plasma s...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Twito T,Chen Z,Khatri I,Wong K,Spaner D,Gorczynski R

    更新日期:2013-07-01 00:00:00

  • Minimally differentiated acute leukemia.

    abstract::We have studied 35 adult patients with morphologically undifferentiated peroxidase-negative acute leukemia that failed to meet the criteria for acute lymphoblastic leukemia and compared them to patients with FAB M1-M7 seen by the same physicians. The diagnosis of minimally differentiated acute leukemia (MD-AL) was ass...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Cadwell FJ,Burns CP,Dick FR,Jones MP,Heckman KD,Weiner GJ,Goeken JA

    更新日期:1993-03-01 00:00:00