Abstract:
:The development of preventive or therapeutic recombinant vaccines and the generation of serodiagnostic assays for infectious diseases depend essentially on the availability of molecularly defined antigens. A major bottleneck for the identification of suitable target antigens for many pathogens is the isolation of sufficient amounts of material for subsequent genomic or proteomic screening. Applying a highly efficient expression cloning strategy to the human pathogens vaccinia virus (VV) and Chlamydia pneumoniae (CP), we demonstrate that sub-nanogram amounts of isolated nucleic acids can be utilized to determine comprehensive sets of immunodominant antigens. Remarkably, the approach not only confirmed the immunogenicity of previously reported antigens but also disclosed novel vaccine candidates conserved in orthopoxviruses, including antigenic envelope proteins and immunodominant CTL epitopes. Moreover, as illustrated for CP infection, we show that a panel of novel antigens can be readily selected from the initially discovered pool to build up pathogen-specific seroassays. The established approach is rapid, making it an attractive procedure for the comprehensive dissection of immunomes of known human pathogens and newly emerging infectious agents.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Sahin U,Türeci O,Graf C,Meyer RG,Lennerz V,Britten CM,Dumrese C,Scandella E,Wölfel T,Ludewig Bdoi
10.1002/eji.200535538keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
1049-57issue
4eissn
0014-2980issn
1521-4141journal_volume
36pub_type
杂志文章abstract::CTLA-4 is a crucial immune regulator that mediates both negative costimulation signals to T cells, and regulatory T (Treg)-cell extrinsic control of effector responses. Here we present evidence supporting a novel mechanism for this extrinsic suppression, executed by the alternatively spliced soluble CTLA-4 isoform (sC...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242529
更新日期:2013-05-01 00:00:00
abstract::The cytokines interleukin (IL)-6, IL-11, ciliary neurotrophic factor (CNTF), leukemia inhibitor factor (LIF), oncostatin M (OSM) and probably the recently cloned cytokine cardiotrophin-1, signal, in combination with their specific receptors, through the common signal transducer gp130. Here, we report that the signalin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251240
更新日期:1995-12-01 00:00:00
abstract::Epidermal keratinocytes differentiate and form a multilayered epidermis, which is the primary barrier between the body and the outer environment. As the epidermis is constantly exposed to a variety of microbial pathogens, its function of resisting microbial pathogens is vital. This characteristic feature is formed dur...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425827
更新日期:2005-06-01 00:00:00
abstract::Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilized multip...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847511
更新日期:2018-09-01 00:00:00
abstract::Haemophilus influenzae type b capsular polysaccharide (PRP) conjugate vaccines, which are thought to induce T cell-dependent antibody production, induce protective responses after a single dose in individuals under 15 months of age. However, multiple doses of these vaccines are required to induce protective antibody r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200108)31:8<2373::aid-immu2373>3
更新日期:2001-08-01 00:00:00
abstract::The distribution of idiotype-positive plaques in mice of various strains injected with T-independent (TI) and T-dependent conjugates of p-azobenzenearsonate (Ars) has been investigated. With maturation of the responses there is a progressive loss of dominance of the major cross-reactive idiotype (CRI) in A/J and C.AL-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120302
更新日期:1982-03-01 00:00:00
abstract::Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3
更新日期:2000-07-01 00:00:00
abstract::We generated cell hybrids between mouse T cell lymphoma EL4 cells and mouse fibroblast B82 cells (BELIII and BELIV) to examine the expression of T cell receptor (TcR)/CD3 genes and their lymphoid-specific transcription factor genes, which are normally detected in EL4 cells. In BELIII and BELIV, expression of the TcR a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250947
更新日期:1995-09-01 00:00:00
abstract::Strains CBA (M523) (= M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B 10.D2 (M504 (= M504) and b 10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CB...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090208
更新日期:1979-02-01 00:00:00
abstract::Cross-linking of surface immunoglobulin (sIg) by antibodies against IgM, IgG and IgD activates B cells and in some circumstances can induce cell proliferation. We studied the potential link between anti-Ig-induced changes in the cytosolic free Ca2+ concentration ([Ca2+]i), inositol phosphate production and the ability...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171209
更新日期:1987-12-01 00:00:00
abstract::We have analyzed the inducibility of protein kinase C (PKC)-dependent expression of CD 69 molecules in T cell receptor (TCR) transgenic thymocytes developing in the presence or absence of selecting, class I major histocompatibility complex (MHC) molecules. Small CD4+8+ thymocytes developing in the absence of selecting...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230326
更新日期:1993-03-01 00:00:00
abstract::Soluble TRAIL (sTRAIL) can be produced by myeloid-derived cells to kill cancer cells. Whether this mechanism is used by T cells, and if so, how sTRAIL production is regulated, remains unclear. Our previous studies showed that ex vivo expanded human γδ T cells express TRAIL and NK receptor group 2 (R2), member D (NKG2D...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201243150
更新日期:2013-12-01 00:00:00
abstract::CD25+ FOXP3+CD4+ T cells (Treg) have been considered to play an important role in immune tolerance against several tumor antigens. It has also been indicated that high-level expression of FOXP3 (FOXP3high) is sufficient to confer suppressive activity to normal non-Treg. Here, we showed for the first time that vascular...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939887
更新日期:2010-01-01 00:00:00
abstract::RNA from 170 different Ig-secreting hybridomas was hybridized with VH gene probes 7183, QUPC52, S107, J558, J606, 36-60, V31, X-24 and V-GAM 3.8, with the aim of comparing xid to non-xid mice, neonatal NZB to adult NZB, and the peritoneal cavity with the splenic compartments for VH gene family expression. Our results ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181217
更新日期:1988-12-01 00:00:00
abstract::The extracellular domain of the lymphocyte surface antigen CD38 has been recently shown to share a high sequence homology with a nicotinamide adenine dinucleotide (NAD+)-specific hydrolyzing enzyme cloned from the ovotestis of the gastropod Aplysia (E. States, D.J., Walseth, T.F., Lee, H. C., Trends Biochem. Sci. 1992...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231245
更新日期:1993-12-01 00:00:00
abstract::Germinal centers support the differentiation of memory B cells and long-lived antibody-secreting cells during infection or upon vaccination. Here, we constructed mice with T cells that selectively lack the chemokine receptor CXCR5 to determine if expression of this receptor by T cells is mandatory for germinal center ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636486
更新日期:2007-01-01 00:00:00
abstract::To determine whether antigen (Ag)-specific cytotoxic T lymphocytes are generated during experimental Candida albicans infection, purified L3T4+ and Ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, C. albicans Ag, and interleukin 2. Yeast-infected bone marrow macrophages...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210636
更新日期:1991-06-01 00:00:00
abstract::We have studied a secondary IgG anti-dinitrophenyl response in dissociated mouse spleen cell cultures which is comparable in magnitude to the responses detected after adoptive transfer. The data indicate that the responsive precursor cell is not a recirculating memory cell but is an intermediate B cell type which appe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060104
更新日期:1976-01-01 00:00:00
abstract::A fusion between the mouse AKR thymoma BW5147 and a culture of homogeneously OX8+ (CD8) rat T lymphoblasts yield interleukin (IL) 2-dependent T cell hybridomas when selected in HAT medium supplemented with IL 2-containing supernatants of concanvalin A-activated cells and dexamethasone. IL 2-independent variants can be...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190704
更新日期:1989-07-01 00:00:00
abstract::An expanding spectrum of acute and chronic non-infectious inflammatory diseases is uniquely responsive to IL-1β neutralization. IL-1β-mediated diseases are often called "auto-inflammatory" and the dominant finding is the release of the active form of IL-1β driven by endogenous molecules acting on the monocyte/macropha...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201141550
更新日期:2011-05-01 00:00:00
abstract::Upon receptor activation, the myeloid C-type lectin receptor Mincle signals via the Syk-CARD9-Bcl10-MALT1 pathway. It does so by recruiting the ITAM-bearing FcεRI-γ. The related receptor macrophage C-type Lectin (MCL) has also been shown to be associated with Syk and to be dependent upon this signaling axis. We have p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343752
更新日期:2013-12-01 00:00:00
abstract::MHC class II tetramers are attractive tools to study antigen-specific CD4(+) T cell responses in various clinical situations in humans. HLA-DRA1*0101/DRB1*0401 MHC class II heterodimers were produced as empty molecules using the Drosophila melanogaster expression system. Peptide binding experiments revealed that these...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425281
更新日期:2004-10-01 00:00:00
abstract::Peptides presented by MHC class I molecules are mostly derived from proteins synthesized by the antigen-presenting cell itself, while peptides presented by MHC class II molecules are predominantly from materials acquired by endocytosis. External antigens can also be presented by MHC class I molecules in a process refe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445156
更新日期:2015-02-01 00:00:00
abstract::Btk is a member of the Tec family of protein tyrosine kinases expressed in B cells. It is stimulated following cross-linking of the B cell receptor which leads to the autophosphorylation of a specific residue in the SH3 domain, Y223. Previous work using Btk-derived fusion proteins has shown that the Btk SH3 domain bin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199907)29:07<2269::AID-IMM
更新日期:1999-07-01 00:00:00
abstract::In this report, we provide new evidence of a crosstalk between T cell activation and adhesion processes through a functional cytokeleton. We show that CD82 signaling induces long-lasting adhesion, spreading and development of membrane extensions, involving actin polymerization. Addition of various co-stimuli (phorbol ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<4332::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::The role of the invariant chain (Ii), an MHC class II-associated chaperone, in B cell development is controversial. Ii deficient mice (Ii(-/-) mice) show a defect in B cell development. This defect has been attributed to the absence of a fragment liberated from the Ii by intramembranous proteolysis. It was proposed th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425246
更新日期:2004-08-01 00:00:00
abstract::We have studied the kinetics of the antigen induced response of naive and memory CD8 T cells expressing a transgenic T cell receptor (TCR) specific for the glycoprotein peptide amino acid 33-41 (GP33) of the lymphocytic choriomeningitis virus (LCMV). Memory T cells were generated in vivo by adoptive transfer of LCMV T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199901)29:01<284::AID-IMMU
更新日期:1999-01-01 00:00:00
abstract::The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324336
更新日期:2004-02-01 00:00:00
abstract::The mechanisms responsible for major histocompatibility complex (MHC)-linked unresponsiveness are still poorly understood. Here we examine the cellular events that follow when B10. A mice are immunized with cow insulin, an antigen to which they make no apparent immunologic response. Despite the fact that there is no d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180122
更新日期:1988-01-01 00:00:00
abstract::The desire to induce HIV-1-specific responses soon after birth to prevent breast milk transmission of HIV-1 led us to propose a vaccine regimen which primes HIV-1-specific T cells using a recombinant Mycobacterium bovis bacillus Calmette-Guérin (rBCG) vaccine. Because attenuated live bacterial vaccines are typically n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141962
更新日期:2011-12-01 00:00:00