Abstract:
:A fusion between the mouse AKR thymoma BW5147 and a culture of homogeneously OX8+ (CD8) rat T lymphoblasts yield interleukin (IL) 2-dependent T cell hybridomas when selected in HAT medium supplemented with IL 2-containing supernatants of concanvalin A-activated cells and dexamethasone. IL 2-independent variants can be selected from cloned IL2-dependent hybrids in the absence of conditioned medium. Karyotype analysis was used to test a previously proposed hypothesis according to which IL2-independent variants arise through loss of a specific cytotoxic T lymphocyte (rat) chromosome carrying a gene responsible for IL2 dependence. Comparison of karyotypes of several independently derived hybrids with those of their IL 2-independent variants showed that the hybrids contain at least one homologue of all rat chromosomes, and that no pair of rat chromosomes is consistently absent in the IL 2-independent variants.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Somoza C,Fernández-Ruiz E,Jotterand-Bellomo M,Sanz E,Nabholz M,Silva Adoi
10.1002/eji.1830190704subject
Has Abstractpub_date
1989-07-01 00:00:00pages
1177-81issue
7eissn
0014-2980issn
1521-4141journal_volume
19pub_type
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