Abstract:
:Two different lines of C57BL/6 mice (IgHb) carrying complete rearranged mu chain genes from BALB/c (IgMa) were analyzed for the expression and secretion of endogenous as well as transgenic immunoglobulins at the level of single cells. Quantitation of B cells expressing endogenous IgMb by cytofluorometry, limiting dilution analyses of clonal precursors and secretory cell assays revealed a marked selective expansion, activation and terminal differentiation of those cells producing endogenous immunoglobulins. Thus, the very infrequent IgMb-bearing B cells produced in bone marrow of transgenic mice accumulate in spleen, where they are activated and account for roughly half of all natural immunoglobulin-secreting cells. These observations indicate that mu-transgenic mice are valuable in studies of the antibody repertoire selection operating in unprimed animals but their use could be misleading in the analyzing "monoclonal" immune system.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Grandien A,Coutinho A,Andersson Jdoi
10.1002/eji.1830200507subject
Has Abstractpub_date
1990-05-01 00:00:00pages
991-8issue
5eissn
0014-2980issn
1521-4141journal_volume
20pub_type
杂志文章abstract::Many lymphocytes are produced in the intestinal mucosa, especially in the Peyer's patches. These newly formed lymphoid cells leave the gut wall, undergo further maturation and many reach the lamina propria of the intestinal mucosa where they function as effector and regulator cells of the intestinal immune response. H...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250336
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abstract::MicroRNA (miRNA) are a class of post-transcriptional regulators of gene expression targeting mRNA for translational repression and/or degradation. We analyzed the expression of 365 miRNA in lymphocytes in relapsing-remitting MS patients, and show the first evidence for distinct miRNA expression profiles in CD4(+), CD8...
journal_title:European journal of immunology
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abstract::Production of indoleamine 2,3-dioxygenase (IDO) by tumor cells, leading to tryptophan depletion and production of immunosuppressive metabolites, may facilitate immune tolerance of cancer. IDO gene is also expressed in dendritic cells (DC) upon maturation induced by lipopolysaccarides or IFN. We investigated IDO gene e...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2020-07-10 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.1830250622
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abstract::Plasma cells secreting IgG, M, and A abound in the synovium of patients with rheumatoid arthritis, yet their immunoglobulin repertoire and clonal relationship remain to be elucidated. Locally synthesized immunoglobulins probably contribute to the chronic joint inflammatory processes which are characteristic of these p...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1986-08-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2002-02-01 00:00:00
abstract::The expression of the Pit-1/GHF-1 transcription factor (hereafter Pit-1), which controls the expression of growth hormone (GH) and prolactin (PRL) in the pituitary gland, has been documented in human and rat hemopoietic and lymphoid tissues and cell lines. Pit-1 mRNA was detected by in situ hybridization in about 1% o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230428
更新日期:1993-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181019
更新日期:1988-10-01 00:00:00
abstract::During mammalian pregnancy, one or more semiallogeneic fetuses gestate in direct contact with the maternal circulation and uterine tissue. However, a damaging maternal immune response is not normally provoked. We studied two possible mechanisms for this maternal-fetal tolerance, alone and in combination. First, we dir...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3479::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::The inbred strain 13 guinea pig expresses at least three isotypes of immunoglobulin (Ig) lambda chain. The proportion of these isotypes in normal Ig can be estimated by isolating a constant (C) region cyanogen bromide peptide from lambda chains and quantitatively analyzing its component tryptic peptides. Anti-phenyltr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081212
更新日期:1978-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838269
更新日期:2008-08-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/1521-4141(200007)30:7<1911::AID-IMMU1911>3
更新日期:2000-07-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.1830210921
更新日期:1991-09-01 00:00:00
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journal_title:European journal of immunology
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abstract::IL-12 and IL-4, respectively, dominate Th1 versus Th2 polarization. Additionally IL-4 can inhibit IL-12 p40 production in DC. Here we show that macrophages respond to bacterial CpG-DNA with IL-12 p40 production in an IL-4-sensitive manner. Analysis of the molecular mechanism of this inhibition shows that IL-4 acts by ...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
abstract::Dermal and mucosal lymphocyte infiltrates in patients with lichen ruber planus and chronic graft-vs.-host disease exhibited reactivity with anti-fibronectin antibodies. This reactivity was associated with the lymphocytes and localized pericellularly. In contrast, biopsy areas outside lymphocyte accumulations were rela...
journal_title:European journal of immunology
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更新日期:1991-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747073
更新日期:2018-01-01 00:00:00
abstract::A significant proportion of memory B cells home to bone marrow (BM) which is a major site of anamnestic antibody responses in mice. We hypothesized that memory T cells likewise accumulate in BM perhaps to provide help for antibody production, and that the compartment of CD4+ T cells in BM of unimmunized mice would be ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199903)29:03<1051::AID-IMM
更新日期:1999-03-01 00:00:00
abstract::IL-12 is a potent inducer of IFN-gamma production and drives the development of Th1 cells. Human polarized Th2 cells do not express the signaling beta2-subunit of the IL-12R and, therefore, do not signal in response to IL-12. The question was raised as to what extent the loss of the IL-12Rbeta2 chain in Th2 cells has ...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
abstract::We have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation...
journal_title:European journal of immunology
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doi:10.1002/eji.1830231122
更新日期:1993-11-01 00:00:00
abstract::Vitamin A and its metabolite retinoic acid influence various aspects of immunity. Although the capacity of vitamin A to condition intestinal CD103(+) DCs to imprint tissue-specific homing programs onto activated lymphocytes is well documented, it is unclear whether vitamin A also regulates DC populations in other tiss...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
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更新日期:2013-06-01 00:00:00
abstract::B14-2-14 is a monoclonal cytotoxic IgM antibody which reacts with thymocytes of all mouse strains tested. The fraction of positive cells (by visual immunofluorescence) varies between strains from about 25-45% in A.CA to 65-85% in C57BL/6, and high levels are dominant in F1 hybrids. In the periphery, the antigen is fou...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:2016-03-01 00:00:00
abstract::IL-18 is involved in the pathogenesis of atopic dermatitis, psoriasis, and allergic contact dermatitis. CXCL9, CXCL10, and CXCL11 recruit type 1 T cells, and the production of these chemokines by keratinocytes is enhanced in these dermatoses. We examined the in vitro effects of IL-18 on IFN-gamma-induced CXCL9, CXCL10...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636420
更新日期:2007-02-01 00:00:00
abstract::CTLA-4 is a crucial immune regulator that mediates both negative costimulation signals to T cells, and regulatory T (Treg)-cell extrinsic control of effector responses. Here we present evidence supporting a novel mechanism for this extrinsic suppression, executed by the alternatively spliced soluble CTLA-4 isoform (sC...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242529
更新日期:2013-05-01 00:00:00