Abstract:
:Many lymphocytes are produced in the intestinal mucosa, especially in the Peyer's patches. These newly formed lymphoid cells leave the gut wall, undergo further maturation and many reach the lamina propria of the intestinal mucosa where they function as effector and regulator cells of the intestinal immune response. However, the number and subset composition of these newly formed lymphocytes emigrating from the gut wall are not known. Therefore, the intestinal lymph duct was cannulated in eight minipigs, in which the mesenteric lymph nodes had been removed 3 months earlier. Thus, it was possible to obtain all lymphocytes leaving the intestinal mucosa including the Peyer's patches via lymphatics. The hourly output of lymphocyte subsets was examined over the course of 93 h. The percentage and the absolute numbers of newly formed T cells (CD2+, CD8+) and B cells (IgA+, IgM+) were determined by examining the incorporation of the DNA precursor bromodeoxyuridine. After a single i.v. bromodeoxyuridine injection 8.5% of the T, 55% of the IgA+ and 25% of the IgM+ cells were labeled. In absolute numbers (1.9 +/- 0.7) x 10(6) newly formed T cells, (0.4 +/- 0.3) x 10(6) IgA+ cells and (0.5 +/- 0.4) x 10(6) IgM+ cells emigrated from the gut wall per hour. Both T and B lymphocyte subpopulations that are produced in the intestinal mucosa leave the gut wall via lymphatics; interesting, the T cells outnumber the B cells. Obviously the induction and maintenance of mucosal immunity depend to a large extent on the function of newly formed T lymphocytes emigrating from the Peyer's patches and/or from the mucosa without Peyer's patches.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Rothkötter HJ,Hriesik C,Pabst Rdoi
10.1002/eji.1830250336subject
Has Abstractpub_date
1995-03-01 00:00:00pages
866-9issue
3eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::Human ILCs are classically categorized into five subsets; cytotoxic CD127- CD94+ NK cells and non-cytotoxic CD127+ CD94- , ILC1s, ILC2s, ILC3s, and LTi cells. Here, we identify a previously unrecognized subset within the CD127+ ILC population, characterized by the expression of the cytotoxic marker CD94. These CD94+ I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048696
更新日期:2020-12-09 00:00:00
abstract::The major histocompatibility complex (MHC)-encoded transporter associated with antigen processing (TAP) delivers cytosolic peptides to the lumen of the endoplasmic reticulum (ER) for presentation by MHC class I molecules. For the rat, it has been demonstrated that TAP polymorphism results in the selection of different...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250808
更新日期:1995-08-01 00:00:00
abstract::Antigen-specific immunotherapy is an attractive strategy for cancer control. In the context of antiviral vaccines, adenoviral vectors have emerged as a favorable means for immunization. Therefore, we chose a strategy combining use of these vectors with another successful approach, namely linkage of the vaccine antigen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939543
更新日期:2009-10-01 00:00:00
abstract::To define activation-specific sequences in human T lymphocytes, a cDNA library was constructed by subtractive hybridization using resting and stimulated peripheral blood lymphocyte pairs. Stimulation of peripheral blood lymphocytes was achieved by triggering with mitogenic anti-CD2 (T11) monoclonal antibodies. Differe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190614
更新日期:1989-06-01 00:00:00
abstract::The presentation of islet antigens in the pancreatic LNs (PLNs) of mice is a developmentally regulated process. It has been hypothesized that, during physiological tissue remodeling, a wave of neonatal β-cell apoptosis may initiate diabetes in autoimmune-prone strains of mice. If true, increasing or decreasing physiol...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141476
更新日期:2011-08-01 00:00:00
abstract::Mercuric chloride (HgCl2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl2; instead, there is immunosuppress...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261018
更新日期:1996-10-01 00:00:00
abstract::Developing T cells undergo distinct selection processes that determine the T cell receptor (TcR) repertoire. One of these processes is positive selection. Positive selection involves the differentiation to mature T cells of thymocytes bearing TcR capable of recognizing antigens in the context of self-major histocompat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220917
更新日期:1992-09-01 00:00:00
abstract::Vaccination of mice with irradiated Schistosoma mansoni larvae confers high levels of immunity which is mediated by Th1-type lymphocytes. To investigate a possible role for IL-12 in the induction of protection, we have compared the immune response of IL-12 p40-deficient (KO) mice and their C57BL/6 (WT) counterparts fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2827::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Alkaline phosphatases (APases, EC 3.1.3.1) are ecto-enzymes bound to cell membranes by a phosphatidyl-inositol anchor. We have previously shown that APase is present on activated murine B cells and its expression correlates with the process of B cell differentiation into immunoglobulin secretion. Recently, a monoclona...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200436
更新日期:1990-04-01 00:00:00
abstract::Subcutaneous immunization with hen egg-white lysozyme (HEL) in complete Freund's adjuvant induces, both in antibody responder and nonresponder mice, a classical delayed-type hypersensitivity (DTH) reaction evaluated as footpad swelling. This response can be specifically transferred to naive recipients by Lyt-1+2- T ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140910
更新日期:1984-09-01 00:00:00
abstract::Amount and affinity of anti-beta-D-galactosidase-activating antibodies (AAb) were studied in two lines of mice obtained by selective breeding for antibody response to heterologous erythrocytes. These lines, referred to as high (H) and low (L) lines, differ also in their responses to many antigens. Concerning AAb amoun...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090312
更新日期:1979-03-01 00:00:00
abstract::Interleukin-12 (IL-12) has been shown to be produced by monocytes by the ligation of CD40. In the present experiments, IL-12 is shown to be produced by murine spleen antigen-presenting cells (APC) by interaction with T helper 1 (Th1) clones through CD40-CD40 ligand (CD40L) interaction, but not with Th2 clones. The IL-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260318
更新日期:1996-03-01 00:00:00
abstract::Membrane-bound immunoglobulins of the IgM and IgD class are expressed on the B cell surface in association with a disulfide-linked heterodimer consisting of alpha and beta subunits. While the alpha component of the IgM antigen receptor (IgM-alpha, 34 kDa) is encoded by the B cell-specific gene mb-1, the gene coding fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211012
更新日期:1991-10-01 00:00:00
abstract::Natural killer (NK) cells in normal mouse spleen have so far been found to lack conventional cell surface markers. Recently, neuraminidase treatment has been shown to uncover receptors for the A hemagglutinin of the snail Helix pomatia (HP) on populations (preferentially T cells) of human blood lymphocytes. We have us...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081103
更新日期:1978-11-01 00:00:00
abstract::The murine intestinal epithelium contains a heterogeneous population of intraepithelial leukocytes (IEL) most of which are granulated, Thy-1-CD5-CD8+. In order to assess the lineage relationship of this subgroup of IEL to peripheral T cells, we examined IEL in mice with the severe combined immunodeficiency (scid/scid)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200327
更新日期:1990-03-01 00:00:00
abstract::Interleukin-HP1 (HP1) is a murine T cell-derived lymphokine, originally described as a growth factor for B cell hybridomas and plasmacytomas, that was recently shown to stimulate growth and differentiation of normal B and T lymphocytes. Here, we describe a cDNA for HP1 that was isolated from a library prepared using m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180202
更新日期:1988-02-01 00:00:00
abstract::The potential of synthetic peptides as vaccines is restricted by their frequent lack of immunogenicity. As with haptens, coupling to a carrier protein is usually required to provide T cell help to anti-peptide antibody-producing B cells. In spite of their short length, a few natural or synthetic peptides are immunogen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170820
更新日期:1987-08-01 00:00:00
abstract::The importance of human herpesvirus 6 (HHV-6) species as human pathogens is increasingly appreciated. However, we do not understand how infection is controlled in healthy virus carriers, and why control fails in patients with disease. Other persistent viruses are under continuous surveillance by antigen-specific T cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242439
更新日期:2012-11-01 00:00:00
abstract::The role of complement receptors on the localization of T and B cells in the spleen of mice was studied using short-term homing experiments in cobra venom factor (CoF)-treated animals. The localization ratio of B and T cells in the spleen of CoF-treated mice decreased significantly compared to control recipients. No c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150708
更新日期:1985-07-01 00:00:00
abstract::Two out of 25 monoclonal anti-DNA autoantibodies that were produced by human-human hybridoma were found to have lymphocytotoxic activity. The antibodies reacted with normal B and T lymphocytes at cold (4 degrees C) as well as at warm (37 degrees C) temperatures. The lymphocytotoxic activity of the monoclonal anti-DNA ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151012
更新日期:1985-10-01 00:00:00
abstract::The molecular mimicry represented by cross-recognition of determinants shared by unrelated antigens by antibodies or T cells is of broad immunological interest. In this study, we analyzed the cross-recognition by CD4+ T cells of a peptide epitope shared by two mycobacterial proteins of diverse sequence, represented by...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251128
更新日期:1995-11-01 00:00:00
abstract::We analyzed the functional outcome of the interaction between tumor-associated macrophages (TAMs) and natural killer (NK) cells. TAMs from ascites of ovarian cancer patients displayed an alternatively activated functional phenotype (M2) characterized by a remarkably high frequency and surface density of membrane-bound...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/eji.201344130
更新日期:2014-06-01 00:00:00
abstract::Natural Killer (NK) cell-based immunotherapy is a promising approach to treat hepatocellular carcinoma (HCC). The mechanisms underlying the regulation of NK cell activity are not completely understood. In this research, we identified the expression of taste receptor type 1 member 1 (T1R1) and taste receptor type 1 mem...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847688
更新日期:2018-12-01 00:00:00
abstract::Human T cells expressing Vgamma9Ndelta2-encoded TCR recognize several nonpeptide phosphoantigens in the absence of major histocompatibility complex restriction. As these cells respond differentially to increasing concentrations of structurally related phosphoantigens, such ligands constitute agonists of different stre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200105)31:5<1628::AID-IMMU1628>3
更新日期:2001-05-01 00:00:00
abstract::The migration of immunocytes within the extracellular matrix (ECM) is influenced by the activation state of the incoming cell and its responses to the presence of chemokines and cytokines. We studied the regulatory role of TGF-beta1 on T cell homing to secondary lymphatic organs, such as the spleen, and chemotaxis wit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<193::AID-IMMU193>3.0
更新日期:2002-01-01 00:00:00
abstract::Gout manifests as recurrent episodes of acute joint inflammation and pain due to the deposition of monosodium urate (MSU) crystals within the affected tissue in a process dependent on NLRP3 inflammasome activation. The synthesis, activation, and release of IL-1β are crucial for MSU-induced inflammation. The current st...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545798
更新日期:2016-01-01 00:00:00
abstract::We identified the presence in the Aleutian skate, Bathyraja aleutica, of two classes of immunoglobulins (Ig), a high molecular weight Ig analogous to mammalian IgM and a low molecular weight Ig, similarly to the spiny rasp skate, Raja kenojei, (Kobayashi, K. et al., Mol. Immunol. 1984. 21: 397), using an immunological...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150916
更新日期:1985-09-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection frequently develops into liver disease and is accompanied by extra-hepatic autoimmune manifestations. The tetraspanin CD81 is a putative HCV receptor as it binds the E2 envelope glycoprotein of HCV and bona fide HCV particles. Here we show that HCV E2 binding to CD81 on human ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200101)31:1<166::aid-immu166>3.0
更新日期:2001-01-01 00:00:00
abstract::Cofilin, an actin-depolymerizing protein, is essential for the functional dynamics of the actin cytoskeleton and for cell viability. In unstimulated human peripheral blood T lymphocytes cofilin is phosphorylated and localized in the cytoplasm. Following co-stimulation through accessory receptors (e.g. CD2 or CD28) - h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2000012)30:12<3422::AID-IMMU3422
更新日期:2000-12-01 00:00:00
abstract::Neurotropic coronavirus infection of mice results in acute encephalomyelitis followed by viral persistence. Whereas cellular immunity controls acute infection, humoral immunity regulates central nervous system (CNS) persistence. Maintenance of serum Ab was correlated with tissue distribution of virus-specific Ab-secre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535123
更新日期:2006-03-01 00:00:00