Abstract:
:Vaccination of mice with irradiated Schistosoma mansoni larvae confers high levels of immunity which is mediated by Th1-type lymphocytes. To investigate a possible role for IL-12 in the induction of protection, we have compared the immune response of IL-12 p40-deficient (KO) mice and their C57BL/6 (WT) counterparts following vaccination. Cultured lymph node cells from KO mice had markedly altered cytokine profiles with significantly decreased production of IFN-gamma increased IL-4. Correspondingly, KO mice had enhanced levels of IgE. After challenge, cells recovered from the lungs of KO mice secreted abundant IL-4 and IL-5 but little IFN-gamma, while flow cytometric and histological analysis of lung cell populations recorded a very high proportion of eosinophils. The levels of protection in KO mice were substantially lower than in their WT counterparts, demonstrating the importance of IL-12 and Th1-mediated immune responses. This conclusion is reinforced by the administration of rIL-12 to KO mice immediately after vaccination which led to increased IFN-gamma and the restoration of protective immunity. Nevertheless, the data also indicated that the limited levels of protection induced in KO mice occur via an IL-12-independent pathway, possibly mediated by Th2 cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Anderson S,Shires VL,Wilson RA,Mountford APdoi
10.1002/(SICI)1521-4141(199809)28:09<2827::AID-IMMsubject
Has Abstractpub_date
1998-09-01 00:00:00pages
2827-38issue
9eissn
0014-2980issn
1521-4141pii
10.1002/(SICI)1521-4141(199809)28:09<2827::AID-IMMjournal_volume
28pub_type
杂志文章abstract::Human immunodeficiency virus binds to CD4 T lymphocytes by interaction between its envelope glycoprotein gp120 and the CD4 molecule. The latter is non-covalently associated with a src-related tyrosine kinase, p56lck. CD4 cross-linking increases the activity of p56lck, leading to phosphorylation of several cellular sub...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230303
更新日期:1993-03-01 00:00:00
abstract::To investigate negative selection events during intrathymic ontogeny, we established T cell receptor (TCR)-transgenic mice [N15tg/RAG-2-/- (H-2b)] expressing a single TCR specific for vesicular stomatitis virus nuclear octapeptide N52-59 (VSV8) in the context of the major histocompatibility complex (MHC) class I molec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270923
更新日期:1997-09-01 00:00:00
abstract::The analysis of an Ia specificity was performed in several H-2 haplotypes by evaluating the affinity of the binding to mouse spleen cells of a monoclonal anti-Ia alloantibody. We used the monoclonal antibody 10-2.16 (Oi et al., Curr. Top. Microbiol. Immunol. 1978. 81: 115.) which recognizes on I-A gene products a publ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110912
更新日期:1981-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211219
更新日期:1991-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747152
更新日期:2018-10-01 00:00:00
abstract::In this study, after immunization with NK cells from a KIR2DS5(+) donor and screening on cell transfectants expressing different members of the killer immunoglobulin-like receptor (KIR) family, we generated a mAb, DF200, reacting with several KIR2D receptors including KIR2DL1/L2/L3, KIR2DS1/S2 and KIR2DS5. By the anal...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110407
更新日期:1981-04-01 00:00:00
abstract::Accumulating evidence suggests that alloreactive memory T cells (Tm) may form a barrier to tolerance induction in large animals and humans due in part to a resistance to suppression by Treg. However, why Tm are resistant to regulation and how the Tm response to an allograft differs from that of naïve T cells, which ar...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040671
更新日期:2010-11-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1992-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2016-10-01 00:00:00
abstract::Immunizations via the i.n. and intravaginal (ivag) routes effectively generate strong genital tract antibody-mediated immunity. To what extent the same is true for T-cell responses is incompletely known. Therefore, we set out to investigate optimal conditions for stimulation of genital tract CD4(+) T-cell responses, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041297
更新日期:2011-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200102)31:2<646::aid-immu646>3.0
更新日期:2001-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090208
更新日期:1979-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200010)30:10<3057::AID-IMMU3057>
更新日期:2000-10-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2007-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170205
更新日期:1987-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170315
更新日期:1987-03-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1986-07-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:2003-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201025
更新日期:1990-10-01 00:00:00
abstract::Immunodominant T cell epitopes from the autoantigen human cartilage glycoprotein 39 have previously been mapped in the context of HLA-DR*0401 and *0402, using mice expressing HLA-DR4 transgenes. We measured the dissociation rates of these epitopes from soluble recombinant DR*0401 and DR*0402 to assess the relationship...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120515
更新日期:1982-05-01 00:00:00
abstract::There are no direct means to study class I MHC presentation in human normal or diseased cells. Using CMV-infected human cells and applying novel mAb that mimic T-cell receptor specificity directed toward the immunogenic epitope of the viral pp65 protein presented on HLA-A2 molecules, we directly imaged the dynamics of...
journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161020
更新日期:1986-10-01 00:00:00