Abstract:
:In this study, after immunization with NK cells from a KIR2DS5(+) donor and screening on cell transfectants expressing different members of the killer immunoglobulin-like receptor (KIR) family, we generated a mAb, DF200, reacting with several KIR2D receptors including KIR2DL1/L2/L3, KIR2DS1/S2 and KIR2DS5. By the analysis of peripheral blood NK cells and in vitro derived NK cell clones, we have demonstrated for the first time that KIR2DS5 is expressed at the cell surface in discrete subsets of NK cells and, after DF200 mAb-mediated engagement, can induce both cytotoxicity and cytokine release. Using co-transfection and co-immunoprecipitation, we found that KIR2DS5 associates with the DAP12 signaling polypeptide. Finally, soluble KIR2DS5-Fc fusion protein does not bind to cell transfectants expressing different HLA-C alleles, suggesting that, if KIR2DS5 does recognize HLA-C molecules, this may only occur in the presence of certain peptides.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Della Chiesa M,Romeo E,Falco M,Balsamo M,Augugliaro R,Moretta L,Bottino C,Moretta A,Vitale Mdoi
10.1002/eji.200838434subject
Has Abstractpub_date
2008-08-01 00:00:00pages
2284-9issue
8eissn
0014-2980issn
1521-4141journal_volume
38pub_type
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