Abstract:
:Cross-linking induced interactions between the membrane form of immunoglobulin (mIg) and the cytoskeletal matrix have been described by several groups. To date, the function of mIgM association with the cytoskeleton is not yet understood. Delineation of the molecular basis of these interactions will be instrumental in elucidating their function. We have previously shown that the Ig alpha/beta heterodimer is not required for ligand-induced mIgM binding to the cytoskeleton. In this study, we have investigated the role of other B cell-specific proteins in mediating these interactions. For this, we expressed mIgM in the non-hematopoietic human cervical carcinoma cell line HeLa S3 and verified the capacity of the surface-expressed IgM to interact with the cytoskeletal matrix upon cross-linking with anti-mu chain antibodies. We show here that only the mIgM molecule itself and no other B cell-specific protein(s) is required in mediating mIgM interactions with actin filaments. In an attempt to determine the cytoskeleton-binding site of mIgM we investigated the role of the cytoplasmic tail of mIgM (KVK) in binding the receptor to actin-based microfilaments. Using mutated forms of mIgM expressed in J558L cells, we show here that KVK plays a role in mediating these interactions. The absence of KVK did not, however, completely abrogate mIgM-cytoskeletal interactions, suggesting that there are additional molecular requirements for the ligand-induced mIgM binding to the cytoskeletal matrix.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Park JY,Jongstra-Bilen Jdoi
10.1002/eji.1830271137subject
Has Abstractpub_date
1997-11-01 00:00:00pages
3001-9issue
11eissn
0014-2980issn
1521-4141journal_volume
27pub_type
杂志文章abstract::Natural killer T (NKT) lymphocytes rapidly produce several cytokines, including IL-4 and IFN-gamma, upon activation, and act as regulatory cells at an early interphase of innate and adaptive immune responses. They have been implicated as important elements in diverse immune responses including the regulation of autoim...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323963
更新日期:2004-01-01 00:00:00
abstract::We have followed the fate of mature CD8+ T cells with a male-specific transgenic T cell receptor after antigenic stimulation with hemopoietic cells in the absence or presence of help. Our data show that mature CD8+ T cells can be deleted after a 3-week period of transient activation and that help, e.g. in the form of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230835
更新日期:1993-08-01 00:00:00
abstract::Hypoxia and glucose deprivation are often observed in the microenvironment surrounding solid tumors in vivo. However, how they interfere with MHC class I antigen processing and CD8(+) T-cell responses remains unclear. In this study, we analyzed the production of antigenic peptides presented by classical MHC class I in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545835
更新日期:2016-04-01 00:00:00
abstract::The T cell receptor (TcR) on CD8+ T lymphocytes recognizes a complex which consists of a major histocompatibility complex (MHC) heavy chain, beta 2-microglobulin (beta 2M), and peptide on the surface of antigen-presenting cells. Mutational analyses have suggested that the TcR recognizes both the alpha 1 and alpha 2 do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231145
更新日期:1993-11-01 00:00:00
abstract::Upon receptor activation, the myeloid C-type lectin receptor Mincle signals via the Syk-CARD9-Bcl10-MALT1 pathway. It does so by recruiting the ITAM-bearing FcεRI-γ. The related receptor macrophage C-type Lectin (MCL) has also been shown to be associated with Syk and to be dependent upon this signaling axis. We have p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343752
更新日期:2013-12-01 00:00:00
abstract::The invariant chain (Ii) binds to newly synthesized major histocompatibility complex (MHC) class II molecules and is targeted to an acidic compartment where it is degraded. To evaluate its role on the conformation and the subcellular distribution of murine MHC class II molecules we have established stable L cell trans...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231218
更新日期:1993-12-01 00:00:00
abstract::A significant proportion of memory B cells home to bone marrow (BM) which is a major site of anamnestic antibody responses in mice. We hypothesized that memory T cells likewise accumulate in BM perhaps to provide help for antibody production, and that the compartment of CD4+ T cells in BM of unimmunized mice would be ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199903)29:03<1051::AID-IMM
更新日期:1999-03-01 00:00:00
abstract::The immune system has developed several regulatory mechanisms to maintain homeostasis of adaptive immune responses. T-cell programmed death (PD)-1 recognition of B7-H1 (PD-L1) expressed on APC and non-lymphoid tissue regulates T-cell activation. We show that B7-H1(-/-) mice exhibit exacerbated proteoglycan (PG)-induce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040690
更新日期:2010-11-01 00:00:00
abstract::A monoclonal antibody (mAb), 3A35, produced against mouse macrophages (M phi) was found to react against certain activated T cells. This mAb, a rat IgM, resulted from a cell fusion between a mouse plasmacytoma and rat lymphocytes immunized against mouse M phi. It bound more avidly to activated than to resident M phi. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170305
更新日期:1987-03-01 00:00:00
abstract::In the past 15 years, it became clear that the symbiotic microbiota has an important impact on the development and regulation of the immune system. Consequently, it is incorrect to interpret a phenotype solely as a direct result of the genotype, without considering the impact of the microbiota. In fact, ignorance of t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048615
更新日期:2020-06-01 00:00:00
abstract::Young (less than 3 months of age) and old (greater than 1 year of age) C.B-17 scid/scid mice were tested for the presence of immunoglobulin in serum and CD3+ T cells in spleen and peritoneal cavity. In all old severe combined immune deficiency (scid) mice tested we found detectable, but very variable levels of serum i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210240
更新日期:1991-02-01 00:00:00
abstract::Site-directed mutagenesis of a recombinant Fc hinge fragment has recently been used to localize the site of the mouse IgG1 (mIgG1) molecule that is involved in the intestinal transfer of recombinant Fc hinge fragments in neonatal mice. This site encompasses Ile-253, His-310, Gln-311, His-433 and Asn-434, localized at ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261038
更新日期:1996-10-01 00:00:00
abstract::Natural resistance to hemopoietic allograft results in functional elimination of the graft from the host within 24 to 48 h. The resistance is specific and is directed to the cell surface target structures of an unidentified nature. These determinants are controlled by the major histocompatibility complex-linked hemopo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190702
更新日期:1989-07-01 00:00:00
abstract::The use of fetal bovine serum (FBS) for the culture and expansion of mesenchymal stromal cells (MSCs) limits their possible clinical applications. Although some recent studies recommended substituting FBS with human platelet lysate (HPL) for the expansion of MSCs for clinical use, the functional capacity of the expand...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141542
更新日期:2011-11-01 00:00:00
abstract::The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night cu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260630
更新日期:1996-06-01 00:00:00
abstract::The magnitude of the IgE antibody response against the phosphorylcholine (PC) hapten and the rate of age-related change of IgE formation is polymorphic in the three long-lived and six relatively short-lived strains of mice studied. Long-lived mice showed an early increase, followed by a decline in the levels of anti-P...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160621
更新日期:1986-06-01 00:00:00
abstract::Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101111
更新日期:1980-11-01 00:00:00
abstract::In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonst...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948474
更新日期:2020-06-01 00:00:00
abstract::Interleukin-HP1 (HP1) is a murine T cell-derived lymphokine, originally described as a growth factor for B cell hybridomas and plasmacytomas, that was recently shown to stimulate growth and differentiation of normal B and T lymphocytes. Here, we describe a cDNA for HP1 that was isolated from a library prepared using m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180202
更新日期:1988-02-01 00:00:00
abstract::To elucidate the rapid destruction of allogeneic bone marrow (BM) grafts in nonsensitized rats we have assessed: (a) the 21-h tissue localization of 51Cr-labeled allogeneic vs. syngeneic BM cells, (b) the ability of allogeneic BM cells to proliferate in the BM or irradiated recipients, (c) the survival of allogeneic v...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140906
更新日期:1984-09-01 00:00:00
abstract::Targeting exogenous antigen into the MHC class I-restricted presentation pathway is a prerequisite for the induction of cytotoxic T lymphocytes (CTL) which have been shown to represent an important component of the protective and therapeutic immune response to viral infections and tumors. In this study, we produced re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2726::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Even after repeated immunizations with alpha(1-3)dextran (Dex) male (CBA/N x BALB/c)F1 mice fail to produce specific antibodies whereas nondefective female littermates express an idiotype-positive anti-Dex immune response. This failure of xid mice to express serum anti-Dex immunoglobulins is not only limited to immuni...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181111
更新日期:1988-11-01 00:00:00
abstract::We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747019
更新日期:2017-08-01 00:00:00
abstract::Reciprocal induction of the Th1 and Th17 immune responses is essential for optimal protection against Mycobacterium tuberculosis (Mtb); however, only a few Mtb antigens are known to fulfill this task. A functional role for resuscitation-promoting factor (Rpf) E, a latency-associated member of the Rpf family, in promot...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445329
更新日期:2015-07-01 00:00:00
abstract::In the murine system, we tested in vivo the immunogenicity of different preparations of the yeast-derived surface antigen (S-antigen or S-protein) of hepatitis B virus (HBV). Native S-protein molecules self-assemble into stable 22-nm particles. BALB/c mice immunized with low doses of native S-particles without adjuvan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240512
更新日期:1994-05-01 00:00:00
abstract::The multimeric Fc gamma RIIIA (CD16) complex is expressed on the surface of natural killer (NK) cells and is composed of a 50-70-kDa transmembrane glycoprotein Fc gamma receptor (CD16), the T cell receptor (TCR)-zeta chain, and the Fc epsilon RI gamma chain. Cross-linking Fc gamma RIIIA initiates the rapid tyrosine ph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231017
更新日期:1993-10-01 00:00:00
abstract::Previous studies have suggested that T-lymphocyte dysfunction might be attributable to nitrative stress induced by reactive nitrogen species (RNS). In this manuscript, we explored this hypothesis and provided a direct demonstration of the inhibitory effects of RNS on human T-cell signaling, activation, and migration. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040868
更新日期:2011-07-01 00:00:00
abstract::Murine Thy-1+, TcR V gamma 3/V delta 1+ dendritic epidermal T cells (DETC) differ from most other T cell subsets by the absence of CD4 and CD8 antigens as well as the lack of CD5 expression. To see whether negativity for those antigens is an intrinsic feature of a given T cell population or if such triple-negative T c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240612
更新日期:1994-06-01 00:00:00
abstract::Mice of strain MRL/Mp-lpr/lpr develop resistance against tolerance induction with aggregate-freed rabbit gamma-globulin as early as 6 weeks of age and show very complete resistance when they are 10 weeks old; congenic mice MRL-Mp+/+ of 6 and 10 weeks can be rendered tolerant. By reconstitution experiments, it was show...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830111113
更新日期:1981-11-01 00:00:00
abstract::The autoimmune disease systemic lupus erythematosus is characterized by loss of tolerance to nuclear Ags and a heightened inflammatory environment, which together result in end organ damage. Lyn-deficient mice, a model of systemic lupus erythematosus, lack an inhibitor of B-cell and myeloid cell activation. This resul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142095
更新日期:2013-02-01 00:00:00