Abstract:
:The use of fetal bovine serum (FBS) for the culture and expansion of mesenchymal stromal cells (MSCs) limits their possible clinical applications. Although some recent studies recommended substituting FBS with human platelet lysate (HPL) for the expansion of MSCs for clinical use, the functional capacity of the expanded cells has only been partially explored. 10% FBS and two other commercial FBS-containing media (MesenCult and MesenPro) were compared with 10% HPL-containing medium for their ability to support MSCs expansion and immunomodulation. We demonstrate that HPL sustained MSC proliferation and expansion in vitro. However, the cumulative cell numbers recovered were comparable with those obtained in MesenPro medium. Moreover, we show that HPL alters the expression of some relevant MSC surface molecules, namely the DNAM-1 ligands PVR and Nectin-2, the NKG2D ligand ULBP3, the adhesion molecules CD49d and αvβ3 and the fibroblast-associated protein. In addition, MSCs cultured in HPL displayed impaired inhibitory capacity on T-cell proliferation to alloantigen and NK-cell proliferation and cytotoxicity. Finally, they showed decreased constitutive PGE2 production while IL-6, IL-8 and RANTES secretion were upregulated. These results imply some limitations in the use of HPL for the expansion of MSCs to be used as immunomodulators in clinical applications.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Abdelrazik H,Spaggiari GM,Chiossone L,Moretta Ldoi
10.1002/eji.201141542subject
Has Abstractpub_date
2011-11-01 00:00:00pages
3281-90issue
11eissn
0014-2980issn
1521-4141journal_volume
41pub_type
杂志文章abstract::In viral meningitis the inflammatory response involves activated T cells and monocytes which are recruited into the subarachnoid space. To identify the chemotactic signals attracting the cells to the site of infection in the meninges, we measured the levels of two CXC chemokines, interferon-gamma (IFN-gamma) inducible...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271004
更新日期:1997-10-01 00:00:00
abstract::Fever is associated with improved survival during infection in endothermic and ectothermic species although the protective mechanisms are largely undefined. Previous studies indicate that fever-range thermal stress increases the binding activity of the L-selectin homing receptor in human or mouse leukocytes, thereby p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636421
更新日期:2007-10-01 00:00:00
abstract::Several sets of data indicate that ICOS regulates cytokine production in activated T cells, but is less effective on naïve T cells. This work evaluates ICOS function in human naïve CD4+ T cells through an assessment of the effect of soluble forms of the ICOS and CD28 physiological ligands on activation driven by anti-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535571
更新日期:2006-10-01 00:00:00
abstract::Splenic T cells were primed, after removal of alloreactive cells, to beef insulin on allogeneic antigen-presenting cells (APC). The fine specificity of in vitro secondary response was tested in combinations H-2b (responder) T cell-H-2k (nonresponder) APC, and vice versa, using separated chains of beef and pork insulin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130810
更新日期:1983-08-01 00:00:00
abstract::MHC-peptide-specific Fab antibodies binding to HLA-A*0201 complexes presenting the wild-type EAAGIGILTV (EAA) or analogue Melan-A 10-mer ELAGIGILTV (ELA) peptide were generated to study efficacy of peptide processing and presentation. None of the selected Fab antibodies detected the naturally processed EAA/HLA-A*0201 ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636545
更新日期:2007-07-01 00:00:00
abstract::Current concepts on the pathogenesis of chronic asthma emphasize the role of several inflammatory cell populations and their respective mediators that interact in a complex network. However, beside inflammatory cells, lymphocytes are also present in asthmatic airways. Although little is known about their involvement i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230404
更新日期:1993-04-01 00:00:00
abstract::In the frog Xenopus, MHC class I antigen presentation and processing genes (the immunoproteasome LMP2 and LMP7 and the transporter TAP1 and TAP2) seem to be closely linked in a primordial organization. Two distinct lineages of class Ia and LMP7 loci were previously identified, thus strongly suggesting co-evolution amo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324207
更新日期:2003-11-01 00:00:00
abstract::T-cell development is a spatially and temporally regulated process, orchestrated by well-defined contributions of transcription factors and cytokines. Here, we identify the noncoding RNA miR-142 as an additional regulatory layer within murine thymocyte development and proliferation. MiR-142 deficiency impairs the expr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201746987
更新日期:2017-07-01 00:00:00
abstract::One of the clear paradoxes in tumor immunology is the fact that cross-presentation of cell-associated tumor antigens to CD8(+) T cells is efficient, yet CTL generation is weak, and tumors continue to grow. We examined, for the first time whether this may be due to alterations in the phenotype or function of cross-pres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940153
更新日期:2010-06-01 00:00:00
abstract::IL-22 is an IFN-IL-10 cytokine family member, which is produced by activated Th1 and NK cells and acts primarily on epithelial cells. Here we demonstrate that IL-22, in contrast to its relative IFN-gamma, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction. Gene exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535503
更新日期:2006-05-01 00:00:00
abstract::ME3738 is a new compound that attenuates liver disease in several models of acute and chronic liver inflammation. We used the concanavalin A (Con A) model to elucidate the molecular mechanisms of ME3738 to block liver cell damage. Pretreatment of BALB/c mice with ME3738 prior to Con A injection resulted in a significa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323651
更新日期:2003-08-01 00:00:00
abstract::IL-7 plays many fundamental roles during murine B lineage development. One reported function is to maintain progenitors in a developmentally immature state by preventing differentiation to the surface IgM (sIgM)(+) stage. Withdrawal of IL-7 from cultures has been shown to lead to increases in mature traits such as RAG...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425400
更新日期:2004-10-01 00:00:00
abstract::Previous studies have shown that IL-10 inhibits the accessory cell functions required for production of IFN-gamma by T cells and NK cells. Our results show that although IL-10 did not induce the production of IFN-gamma by NK cells, it did enhance the ability of IL-18 to stimulate NK cell production of IFN-gamma. In ad...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199909)29:09<2658::AID-IMM
更新日期:1999-09-01 00:00:00
abstract::We have shown previously that complement factor H (CFH) and complement factor B (CFB) are constitutively expressed by retinal pigment epithelial cells and their production is regulated by inflammatory cytokines, suggesting that the alternative pathway (AP) of complement activation might play a role in retinal inflamma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040323
更新日期:2010-10-01 00:00:00
abstract::Global transcriptional technologies have revolutionised the study of lymphoid cell populations, but human γδ T lymphocytes specific for phosphoantigens remain far less deeply characterised by these methods despite the great therapeutic potential of these cells. Here we analyse the transcriptome of circulating TCRVγ(+)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141870
更新日期:2012-01-01 00:00:00
abstract::To estimate the extent of the TRG gamma variable (V) gene repertoire used in human T cell ontogeny, we have analyzed the variety of V gamma gene rearrangements in a large series of T and non-T acute and chronic leukemias. A limited heterogeneity of rearranged fragments was observed: only 13 types of differently rearra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180126
更新日期:1988-01-01 00:00:00
abstract::We show that, in vitro, the response of thymocytes to certain stimuli, and their survival largely depend on the nature of the culture environment, i.e. whether thymocytes are stimulated within intact thymus lobes or in cell suspension. Exposure of isolated thymocytes to 12-O-tetra-decanoylphorbol 13-acetate (TPA)+iono...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221011
更新日期:1992-10-01 00:00:00
abstract::Longitudinal studies on the kinetics of viral antigen specific CD8 T cell responses have led to a model whereby a relatively small subset of the primary effector CD8 T cells expanding after the first week of acute viral infection initiate a program of cell survival and differentiation into long lived memory T cells. T...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200535516
更新日期:2005-11-01 00:00:00
abstract::Plasmid cassette-transfer vectors pBHuC chi and pBHuC gamma l have been designed which enable the construction of recombinant baculoviruses directing the co-expression of complete immunoglobulin in insect cells. We describe the application of these vectors for the expression of a human/mouse chimeric monoclonal antibo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250731
更新日期:1995-07-01 00:00:00
abstract::We previously showed that topical flagellin induces profound mucosal innate protection in the cornea against microbial infection, a response involving multiple genes and cell types. In this study, we used a Candida albicans (CA)-C57BL/6 mouse keratitis model to delineate the contribution of CXCL10- and CXCR3-expressin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444490
更新日期:2014-09-01 00:00:00
abstract::Aging is accompanied by altered immunity, resulting in a variable state of poorly understood immunodeficiency. While both the numbers and the functionality of naïve T cells are decreased by aging, the impact of these changes upon immune defense against bacterial pathogens in vivo remains understudied. Using a model of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041141
更新日期:2011-05-01 00:00:00
abstract::The TNF member LIGHT also known as TL4 or TNFSF14) can play a major role in cancer control via its two receptors; it induces tumor cell death through lymphotoxin-beta receptor (LT-betaR) and ligation to the herpes virus entry mediator (HVEM) amplifies the immune response. By studying the effect of LIGHT in the transcr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939069
更新日期:2009-09-01 00:00:00
abstract::NKR-P1 is a type II transmembrane protein which acts as an activation receptor on natural killer (NK) cells. The cytoplasmic domains of the CD4, CD8 and 4-1BB receptors contain the sequence Cys-X-Cys-Pro which is directly involved in coupling to another pair of cysteines in the N-terminal domain of the src family tyro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270111
更新日期:1997-01-01 00:00:00
abstract::The CD38 antigen displays restricted functional associations with surface molecules involved in immune system and complement. Capping of the CD38 molecule in normal or neoplastic T cells is followed by rapid and specific co-modulation of the CD3-T cell receptor (TcR) complex. In normal and tumor cells of B lineage, CD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231005
更新日期:1993-10-01 00:00:00
abstract::The analysis of an Ia specificity was performed in several H-2 haplotypes by evaluating the affinity of the binding to mouse spleen cells of a monoclonal anti-Ia alloantibody. We used the monoclonal antibody 10-2.16 (Oi et al., Curr. Top. Microbiol. Immunol. 1978. 81: 115.) which recognizes on I-A gene products a publ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110912
更新日期:1981-09-01 00:00:00
abstract::R24 is a monoclonal antibody directed against the cell surface ganglioside GD3. It can detect GD3 on the surface of a subset of T lymphocytes and can stimulate proliferation and secretion of cytokines in vitro. In the present report, we examined the effects of the R24 antibody upon antigen-specific T cell response, em...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260928
更新日期:1996-09-01 00:00:00
abstract::The genetic control of in vitro primary syngeneic sensitization on monolayers of thyroid epithelial cells was investigated both at the level of the responder lymphocytes and that of stimulator thyroid epithelial cells using congenic resistant strains of mice. It clearly appears that similar to the in vivo model of exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131115
更新日期:1983-11-01 00:00:00
abstract::LL-37, derived from human cathelicidin, stimulates immune responses in neutrophils. Although FPR2 and P2X7 were proposed as LL-37 receptors, we have shown that among 21 neutrophil receptors only CXCR2 was down-regulated by LL-37. LL-37 functions similarly to CXCR2-specific chemokines CXCL1 and CXCL7 in terms of recept...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939496
更新日期:2009-11-01 00:00:00
abstract::The murine T cell response to heterologous insulins provides a good model system for studying the mechanism of immune response (Ir)-gene function, since insulin is a small, chemically well-defined molecule. H-2b mice respond predominantly to A chain loop determinants of beef insulin, presented by the I-A epitope Ia. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141208
更新日期:1984-12-01 00:00:00
abstract::Group 2 innate lymphoid cells (ILC2s) are innate effectors playing an important role in the defense against helminthic infections and in the pathogenesis of allergic inflammation. Cytokines have been identified as the major stimuli driving ILC2 activation and expansion. Conversely, it is unclear whether costimulatory ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545635
更新日期:2015-10-01 00:00:00