MicroRNA-142 controls thymocyte proliferation.


:T-cell development is a spatially and temporally regulated process, orchestrated by well-defined contributions of transcription factors and cytokines. Here, we identify the noncoding RNA miR-142 as an additional regulatory layer within murine thymocyte development and proliferation. MiR-142 deficiency impairs the expression of cell cycle-promoting genes in mature mouse thymocytes and early progenitors, accompanied with increased levels of cyclin-dependent kinase inhibitor 1B (Cdkn1b, also known as p27Kip1 ). By using CRISPR/Cas9 technology to delete the miR-142-3p recognition element in the 3'UTR of cdkn1b, we confirm that this gene is a novel target of miR-142-3p in vivo. Increased Cdkn1b protein expression alone however was insufficient to cause proliferation defects in thymocytes, indicating the existence of additional critical miR-142 targets. Collectively, we establish a key role for miR-142 in the control of early and mature thymocyte proliferation, demonstrating the multifaceted role of a single miRNA on several target genes.


Eur J Immunol


Mildner A,Chapnik E,Varol D,Aychek T,Lampl N,Rivkin N,Bringmann A,Paul F,Boura-Halfon S,Hayoun YS,Barnett-Itzhaki Z,Amit I,Hornstein E,Jung S




Has Abstract


2017-07-01 00:00:00












  • B cells activated via CD40 and IL-4 undergo a division burst but require continued stimulation to maintain division, survival and differentiation.

    abstract::T cell stimulation of B cell proliferation during T-B collaboration requires membrane-bound stimulatory ligands, such as CD40 ligand and the secretion of soluble cytokines, such as IL-4. Nevertheless, it remains unclear whether T cell contact is required to provoke each consecutive B cell division, or whether B cells ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Rush JS,Hodgkin PD

    更新日期:2001-04-01 00:00:00

  • Interaction of cutaneous stromal cells and gamma/delta T cell receptor (TcR)-positive cells. I. V gamma 5-gamma/delta TcR+ T cells migrating from organ-cultured murine skin proliferate by co-culture with cutaneous stromal cells in the presence of interleu

    abstract::It has been reported that Thy-1+CD3+CD4-CD8- cells as well as Langerhans cells migrate from organ-cultured murine skin into culture medium. We examined whether these Thy-1+ populations of migrating cells were derived from Thy-1+ dendritic epidermal T cells (Thy-1+ DEC) and found that they were Thy-1+CD3+CD4-CD8-gamma/...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Nakagawa S,Hara M,Seki M,Yagita H,Tagami H,Aiba S

    更新日期:1993-07-01 00:00:00

  • Biochemical properties of MHC class II molecules endogenously synthesized and expressed by mouse Langerhans cells.

    abstract::The cell surface expression and biosynthesis of Langerhans cells (LC)-derived major histocompatibility complex (MHC) class II molecules from epidermal cells (EC) prepared freshly and cultured for up to 3 days was investigated. Based on the constitutive expression of MHC class II determinants by LC, a panning and magne...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Becker D,Reske-Kunz AB,Knop J,Reske K

    更新日期:1991-05-01 00:00:00

  • A novel human HER2-derived peptide homologous to the mouse K(d)-restricted tumor rejection antigen can induce HLA-A24-restricted cytotoxic T lymphocytes in ovarian cancer patients and healthy individuals.

    abstract::A mouse HER2-derived peptide, HER2p63 (A) (TYLPANASL), can induce K(d)-restricted mouse cytotoxic T lymphocytes (CTL) and also function as a tumor rejection antigen in an in vivo assay. Since the anchor motif of mouse K(d) for peptide binding has much similarity to that of human HLA-A2402, we asked if human HER2p63 (T...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Okugawa T,Ikuta Y,Takahashi Y,Obata H,Tanida K,Watanabe M,Imai S,Furugen R,Nagata Y,Toyoda N,Shiku H

    更新日期:2000-11-01 00:00:00

  • T cell clones interacting with accessory and T helper cells for a proliferative response.

    abstract::The requirement for cell interactions in T cell activation has been studied with two continuously in vitro growing T cell clones. These clones are specific for minor histocompatibility antigens, are H-2K restricted, and one clone is functionally a cytolytic T lymphocyte. Both can proliferate when interleukin 2 is adde...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Di Pauli R,Brückner T

    更新日期:1984-10-01 00:00:00

  • Identification and characterization of lymphoid precursors in the murine intestinal epithelium.

    abstract::Although in vivo evidence supports a role for the murine intestinal epithelium in the extrathymic generation of certain intraepithelial T lymphocytes (IEL), no intraepithelial cells with in vitro lymphoid progenitor potential have yet been demonstrated. Using reaggregate fetal thymic organ culture techniques, we show ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Woodward J,Jenkinson E

    更新日期:2001-11-01 00:00:00

  • Human antibodies from phage libraries: neutralizing activity against human immunodeficiency virus type 1 equally improved after expression as Fab and IgG in mammalian cells.

    abstract::Human antibodies against HIV-1 have been sought to study neutralization events on the molecular level, and for possible use in passive immune intervention. The development of phage display techniques has opened the possibility of rapidly generating human monoclonal antibodies with desired specificities. We and others ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Samuelsson A,Yari F,Hinkula J,Ersoy O,Norrby E,Persson MA

    更新日期:1996-12-01 00:00:00

  • NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules.

    abstract::Using a new mAb, 2F1, we characterize a mouse natural killer (NK) cell antigen termed 'killer cell lectin-like receptor G1' (KLRG1; formerly mouse MAFA or 2F1-Ag). KLRG1 is expressed on 30-60% of murine NK cells, and a small fraction of T cells, and is composed of a homodimer of glycosylated 30-38-kDa subunits. Striki...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Corral L,Hanke T,Vance RE,Cado D,Raulet DH

    更新日期:2000-03-01 00:00:00

  • ICOS regulates the pool of group 2 innate lymphoid cells under homeostatic and inflammatory conditions in mice.

    abstract::Group 2 innate lymphoid cells (ILC2s) are innate effectors playing an important role in the defense against helminthic infections and in the pathogenesis of allergic inflammation. Cytokines have been identified as the major stimuli driving ILC2 activation and expansion. Conversely, it is unclear whether costimulatory ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Paclik D,Stehle C,Lahmann A,Hutloff A,Romagnani C

    更新日期:2015-10-01 00:00:00

  • Accumulation and activation of epidermal γδ T cells in a mouse model of chronic dermatitis is not required for the inflammatory phenotype.

    abstract::Chronic skin inflammation resulting from a defective epidermal barrier is a hallmark of atopic dermatitis (AD). We previously demonstrated that mice lacking FGF receptors 1 and 2 in keratinocytes (K5-R1/R2 mice) develop an AD-like chronic dermatitis as a result of an impaired epidermal barrier. Here, we show that γδ T...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Sulcova J,Maddaluno L,Meyer M,Werner S

    更新日期:2015-09-01 00:00:00

  • Evidence for Th2 cell-mediated suppression of antibody responses in transgenic, beef insulin-tolerant mice.

    abstract::Clonal deletion, anergy and suppression have all been considered mechanisms of immunological tolerance. Although adoptive transfer of immunosuppression has been shown to occur in the periphery, particularly for transplantation tolerance, it has proven difficult to characterize this phenomenon further, due to the lack ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Teng YT,Gorczynski RM,Iwasaki S,Williams DB,Hozumi N

    更新日期:1995-09-01 00:00:00

  • The generation of LMP2a-specific cytotoxic T lymphocytes for the treatment of patients with Epstein-Barr virus-positive Hodgkin disease.

    abstract::Based upon the success of using polyclonal, Epstein-Barr virus (EBV)-specific CTL lines for the prophylaxis and treatment of patients with post-transplant lymphoproliferative disease (PTLPD), there is now considerable incentive to develop CTL directed against the sub-dominant EBV antigens EBNA1, LMP1 and LMP2, which a...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Su Z,Peluso MV,Raffegerst SH,Schendel DJ,Roskrow MA

    更新日期:2001-03-01 00:00:00

  • Correlated expression of VH framework and VH idiotypic determinants on T helper cells and on functionally undefined T cells binding group A streptococcal carbohydrate.

    abstract::Antibodies to framework determinants of the VH and V lambda fragments of MOPC 315 and antisera to the VH idiotype determinants of the A 5 A antibody were used to analyze the antigen receptors of mouse T (and B) cells. This was done by using the antibodies as inhibitors in (a) an assay in which the binding of radiolabe...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Eichmann K,Ben-Neriah Y,Hetzelberger D,Polke C,Givol D,Lonai P

    更新日期:1980-02-01 00:00:00

  • Dendritic cell-derived IL-15 controls the induction of CD8 T cell immune responses.

    abstract::The development and the differentiation of CD8(+) T cells are dependent on IL-15. Here, we have studied the source and mechanism of how IL-15 modulates CD8(+) T cell-mediated Th1 immune responses by employing two delayed-type hypersensitivity (DTH) models. IL-15-deficient (IL-15(-/-)) mice or mice treated with soluble...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Rückert R,Brandt K,Bulanova E,Mirghomizadeh F,Paus R,Bulfone-Paus S

    更新日期:2003-12-01 00:00:00

  • Dominance of the BV17 element in nickel-specific human T cell receptors relates to severity of contact sensitivity.

    abstract::Hypersensitivity to nickel (Ni) represents the most common manifestation of contact allergy in humans. The role of metal-specific T cells in this disease is well established, but the molecular interactions involved in their activation are poorly understood. We examined the T cell receptor (TCR) repertoire in T cells a...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Vollmer J,Fritz M,Dormoy A,Weltzien HU,Moulon C

    更新日期:1997-08-01 00:00:00

  • Syntaxin 11 is required for NK and CD8⁺ T-cell cytotoxicity and neutrophil degranulation.

    abstract::Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtain...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: D'Orlando O,Zhao F,Kasper B,Orinska Z,Müller J,Hermans-Borgmeyer I,Griffiths GM,Zur Stadt U,Bulfone-Paus S

    更新日期:2013-01-01 00:00:00

  • Peptide variants reveal how antibodies recognize major histocompatibility complex class I.

    abstract::The T cell receptor (TcR) on CD8+ T lymphocytes recognizes a complex which consists of a major histocompatibility complex (MHC) heavy chain, beta 2-microglobulin (beta 2M), and peptide on the surface of antigen-presenting cells. Mutational analyses have suggested that the TcR recognizes both the alpha 1 and alpha 2 do...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Hogquist KA,Grandea AG 3rd,Bevan MJ

    更新日期:1993-11-01 00:00:00

  • Human anti-FcepsilonRIalpha autoantibodies isolated from healthy donors cross-react with tetanus toxoid.

    abstract::Natural antibodies (Ab) reacting with self antigens have been shown to be present in all individuals. These autoantibodies (auto-Ab) can be either pathogenic or non-pathogenic. Auto-Ab reacting with the alpha-subunit of the high-affinity receptor for IgE (FcepsilonRIalpha) have been implicated in the pathogenesis of a...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Horn MP,Gerster T,Ochensberger B,Derer T,Kricek F,Jouvin MH,Kinet JP,Tschernig T,Vogel M,Stadler BM,Miescher SM

    更新日期:1999-04-01 00:00:00

  • Adenovirus 5- and 35-based immunotherapy enhances the strength but not breadth or quality of immunity during chronic SIV infection.

    abstract::Heterologous adenovirus-based vectors hold promise as preventative HIV vaccines but their capacity to induce effective T-cell immunity in established infection has not been explored. We vaccinated rhesus macaques chronically infected with SIVmac251 and undergoing antiretroviral therapy (ART) with human adenovirus sero...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Soloff AC,Liu X,Gao W,Day RD,Gambotto A,Barratt-Boyes SM

    更新日期:2009-09-01 00:00:00

  • Studies on the mechanism of polyclonal B cell stimulation by TH2 cells.

    abstract::Recently, it has been shown that cloned L1/1 T helper cells of type 2 (TH2-cells), when stimulated with antigen, are able to induce polyclonal B cell proliferation. Here we present evidence that this process is dependent on direct cell-cell interaction between T and B cells, which in the effector phase, i.e., during s...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Lohoff M,Dirks M,Rohwer P,Röllinghoff M

    更新日期:1989-01-01 00:00:00

  • Comprehensive analysis of HIV Gag-specific IFN-gamma response in HIV-1- and HIV-2-infected asymptomatic patients from a clinical cohort in The Gambia.

    abstract::Majority of HIV-2-infected individuals meet the criteria of long-term non-progressors. This has been linked to superior qualitative HIV-2-specific cellular immune responses that correlate with viral control. However, it is unknown whether this is due to frequent targeting of immunodominant Gag epitopes in HIV-2 than H...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Ondondo BO,Rowland-Jones SL,Dorrell L,Peterson K,Cotten M,Whittle H,Jaye A

    更新日期:2008-12-01 00:00:00

  • Induction of cell death in T lymphocytes by invasin via beta1-integrin.

    abstract::Ligand binding to beta1-integrins exerts multiple effects on cells of the immune system including adhesion, spreading, haptotaxis and costimulation of T cells activated by anti-CD3. Here we show that a high-affinity ligand for beta1-integrins, the invasin (Inv) protein of Yersinia pseudotuberculosis, can induce cell d...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Arencibia I,Frankel G,Sundqvist KG

    更新日期:2002-04-01 00:00:00

  • Presence of T 145 on cytolytic T cell lines and their lectin-resistant mutants.

    abstract::Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural r...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Conzelmann A,Pink R,Acuto O,Mach JP,Dolivo S,Nabholz M

    更新日期:1980-11-01 00:00:00

  • Peroxisome proliferator-activated receptor gamma activators affect the maturation of human monocyte-derived dendritic cells.

    abstract::Peroxisome proliferator-activated receptor gamma (PPARgamma ), a member of the nuclear receptor superfamily, has recently been described as a modulator of macrophage functions and as an inhibitor of T cell proliferation. Here, we investigated the role of PPARgamma in dendritic cells (DC), the most potent antigen-prese...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Gosset P,Charbonnier AS,Delerive P,Fontaine J,Staels B,Pestel J,Tonnel AB,Trottein F

    更新日期:2001-10-01 00:00:00

  • Expression of two T cell receptor alpha chains on the surface of normal murine T cells.

    abstract::We have previously reported that a subset of T cells in T cell receptor (TCR)-transgenic mice may express two different alpha chains on their surface. The expression of two functional alpha chains has also been demonstrated for human peripheral blood T cells. In this report, we show that a proportion of normal murine ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Heath WR,Carbone FR,Bertolino P,Kelly J,Cose S,Miller JF

    更新日期:1995-06-01 00:00:00

  • De novo polyamine synthesis supports metabolic and functional responses in activated murine NK cells.

    abstract::Cellular metabolism is dynamically regulated in NK cells and strongly influences their responses. Metabolic dysfunction is linked to defective NK cell responses in diseases such as obesity and cancer. The transcription factors, sterol regulatory element binding protein (SREBP) and cMyc, are crucial for controlling NK ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: O'Brien KL,Assmann N,O'Connor E,Keane C,Walls J,Choi C,Oefner PJ,Gardiner CM,Dettmer K,Finlay DK

    更新日期:2020-09-18 00:00:00

  • Comparison of three different forms of HLA-DR4Dw4 proteins.

    abstract::The biochemical behavior and peptide binding properties of a soluble form of the human class II DR4Dw4 molecule (PI-DR4Dw4) were compared to DR4Dw4 molecules containing the transmembrane and cytoplasmic domains that were purified both from B and transfected chinese hamster ovary cells. Recombinant and B cell-derived D...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Buelow R,Paborsky LR,van Schooten WC,Kummerehl TJ,Schreifels R,Marshall K,Mayer J,Rothbard JB

    更新日期:1993-01-01 00:00:00

  • Incomplete effector/memory differentiation of antigen-primed CD8+ T cells in gene gun DNA-vaccinated mice.

    abstract::DNA vaccination is an efficient way to induce CD8+ T cell memory, but it is still unclear to what extent such memory responses afford protection in vivo. To study this, we induced CD8+ memory responses directed towards defined viral epitopes, using DNA vaccines encoding immunodominant MHC class I-restricted epitopes o...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Bartholdy C,Stryhn A,Hansen NJ,Buus S,Thomsen AR

    更新日期:2003-07-01 00:00:00

  • Identification of naturally processed CD8 T cell epitopes from prostein, a prostate tissue-specific vaccine candidate.

    abstract::Prostein is a prostate tissue-specific protein that is uniquely and abundantly expressed in normal and cancerous prostate tissues. Due to this expression profile, we examined the immunogenicity of prostein as a potential vaccine candidate for prostate cancer. To determine the presence of CD8 T cells specific for natur...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Friedman RS,Spies AG,Kalos M

    更新日期:2004-04-01 00:00:00

  • Division of human helper T cells into two sets on the basis of the induction of anti-tumor cytotoxicity by phorbol ester and calcium ionophore.

    abstract::Fourteen noncytotoxic human helper T cell clones were examined for autocrine proliferative responses and cytotoxicity to tumor cells after stimulation with 12-O-tetradecanoylphorbol 13-acetate (TPA) and ionomycin (Io). Although all clones responded to alloantigen, they could be divided into two groups based on their p...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Pawelec G,Busch F,Reinhold U,Rehbein A,Balko I,Bühring HJ

    更新日期:1988-07-01 00:00:00