Abstract:
:Transgenic mice in which mouse interleukin (IL)-7 cDNA is expressed under the control of the mouse major histocompatibility complex (MHC) class II (E alpha) promoter develop a lymphoproliferative disease characterized by the early polyclonal expansion of T cells followed in many cases by the development of lymphomas of immature B cells. Here, we have analyzed B cell development in these transgenic mice. Phenotypic analysis using monoclonal antibodies to B220, IgM, IgD, c-kit, IL-7 receptor, MHC class II, AA4.1, CD19, CD23, CD25, CD40 and CD43 shows that B lymphopoiesis in the bone marrow is dramatically altered and the number of pro/pre-B and immature B cells is significantly increased. Interestingly, pro/pre-B and immature B cells persist in the spleens of adult transgenic mice and are also present in lymph nodes and blood. Cell cycle analysis of lymph node cells shows that subpopulations of developing B cells retain the cell cycle profiles of their bone marrow counterparts. Limiting dilution analysis shows that the number of clonable pre-B cells is significantly increased and that at limiting dilution, growth of transgenic pre-B cells is still dependent on exogenous IL-7. Using semiquantitative polymerase chain reaction (PCR) and in situ hybridization, the level of IL-7 transcripts in the spleen was found to decrease between 2 and 4 weeks in control mice with levels in transgenics mice being approximately 50 times greater. These transgenic mice represent an interesting model with which to study the effects of IL-7 overexpression in the bone marrow and raise interesting questions regarding the regulation of B lymphopoiesis in normal mice.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Mertsching E,Grawunder U,Meyer V,Rolink T,Ceredig Rdoi
10.1002/eji.1830260105subject
Has Abstractpub_date
1996-01-01 00:00:00pages
28-33issue
1eissn
0014-2980issn
1521-4141journal_volume
26pub_type
杂志文章abstract::The germ-line V gene segments encoding the subgroups A and G of the BALB/c V kappa 21 family were cloned and assigned to the previously described 30-kb cluster of the V kappa 21 family. Sequence comparison revealed close homology between the two gene segments at the DNA and the predicted amino acid sequence level, ind...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191031
更新日期:1989-10-01 00:00:00
abstract::CD16-positive (CD14(++) CD16(+) and CD14(+) CD16(++) ) monocytes have unique features with respect to phenotype and function. We have used transcriptional profiling for comparison of CD16-positive monocytes and classical monocytes. We show herein that 187 genes are greater than fivefold differentially expressed, incl...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/eji.201141907
更新日期:2012-04-01 00:00:00
abstract::To investigate the role of membrane lymphotoxin (LT)alpha1 / beta2 and its LTbeta receptor (LTbetaR) in the protective immune response to Mycobacterium bovis bacillus Calmette-Guérin (BCG) infection, we have used a soluble fusion molecule (LTbetaR-IgG1). LTbetaR-Ig treatment interferes with granuloma formation mainly ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4002::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::Growing evidence suggests that some immune responses are mediated not only by conventional and distinct NK cells and CTL, but also by T cell subsets expressing NK receptors and NK cell-associated molecules. Consistent with our previously published finding that the mAb 1F8 identifies non-T/non-B cells in Xenopus that e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1574::AID-IMMU1574>3
更新日期:2002-06-01 00:00:00
abstract::We isolated immunoglobulin (Ig) VH4 genes that were rearranged in the genomic DNA of 160 day human fetal spleen. Productively rearranged VH 4-21 genes were cloned into pRTM1, a human IgM expression vector. This allowed us to generate IgM kappa-expressing transfectomas by co-transfecting each of these constructs with p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241204
更新日期:1994-12-01 00:00:00
abstract::TCR degeneracy may facilitate self-reactive T cell activation and the initiation of an autoimmune response in multiple sclerosis (MS). MHC class II alleles of the DR2 haplotype DR2a (DRB5*0101) and DR2b (DRB1*1501) are associated with an increased risk for MS in Caucasian populations. In order to selectively expand an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737519
更新日期:2008-05-01 00:00:00
abstract::The aim of this study was to investigate the effects of IL-17-producing T cells, including Th17 and Tc17 cells, on acute graft-versus-host disease (aGVHD) in patients who had undergone granulocyte colony-stimulating factor (G-CSF)-mobilised peripheral blood progenitor cell (PBPC) and G-CSF-primed bone marrow (G-BM) tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040793
更新日期:2011-02-01 00:00:00
abstract::Missing-self-reactivity can be mimicked by blocking self-specific inhibitory receptors on NK cells, leading to increased rejection of syngeneic tumor cells. Using a mouse model, we investigated whether Ab-mediated blocking of inhibitory receptors, to a degree where NK cells rejected syngeneic tumor cells, would still ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939755
更新日期:2010-03-01 00:00:00
abstract::Alpha-galactosylceramide (alpha-GalCer) activates invariant (i)NKT cells, which in turn stimulate immunocompetent cells. Although activation of iNKT cells appears critical for regulation of immune responses, it remains elusive whether protection against intracellular bacteria can be induced by alpha-GalCer. Here, we s...
journal_title:European journal of immunology
pub_type: 杂志文章,收录出版
doi:10.1002/eji.200939594
更新日期:2010-05-01 00:00:00
abstract::In human T lymphocytes a functional complex is formed between the protein tyrosine phosphatase CD45, the protein tyrosine kinase p56lck and a phosphoprotein, pp32, a possible common substrate. Here we demonstrate that the previously described pp32 protein is composed of two distinct molecules (pp29 and pp32) in both r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220727
更新日期:1992-07-01 00:00:00
abstract::Adenosine monophosphate-activated protein kinase (AMPK) is a serine/threonine kinase and is crucial for cellular energy homeostasis. The exact role of AMPK during memory CD8(+) T-cell differentiation, a process that changes from the metabolically active state of effector T cells to one of quiescence in memory cells is...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201343483
更新日期:2013-04-01 00:00:00
abstract::Aging is accompanied by altered immunity, resulting in a variable state of poorly understood immunodeficiency. While both the numbers and the functionality of naïve T cells are decreased by aging, the impact of these changes upon immune defense against bacterial pathogens in vivo remains understudied. Using a model of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041141
更新日期:2011-05-01 00:00:00
abstract::Viruses encode immune evasion mechanisms to survive in the immunocompetent host. Chemokines mediate the migration of immune cells and their critical role in immunity is emphasized by the numerous virus-encoded strategies to modulate their activity. Evidence published in this issue of the European Journal of Immunology...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737215
更新日期:2007-04-01 00:00:00
abstract::The interaction between activated T cells and eosinophils has been proposed to play an important role in the pathogenesis of allergic diseases. T cell-derived cytokines such as interleukin-5 and granulocyte/macrophage colony-stimulating factor inhibit eosinophil apoptosis and may therefore contribute to the developmen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260817
更新日期:1996-08-01 00:00:00
abstract::Although the role of interleukin 2 (IL 2) in mature T lymphocyte function is well documented, its effect on hematopoietic progenitor cells is less well characterized. Here we have used recombinant retroviruses to transduce and express a cDNA clone encoding the p55 component of the human IL 2 receptor (h-p55), in a mur...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200317
更新日期:1990-03-01 00:00:00
abstract::Restriction fragment length polymorphism has been compared between the Igh loci of C57BL/6 and MOLF/EI (Mus musculus molossinus) mice utilizing probes which detect the C gamma 2b gene and genes from nine VH-gene families. Distinct restriction site patterns were found for the CH genes and for VH families PC7183, Q52 an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170920
更新日期:1987-09-01 00:00:00
abstract::We have identified a novel subset of early B lineage cells in the mouse bone marrow (BM) by GL7 expression on cell surface. GL7(+)B220(low) BM cells have a large cell size and are CD43(-to low), CD95(-), Sca-1(-), I-A(low), IgM(-) and IgD(-), suggesting that they are large pre-B cells. These BM cells express lambda5 a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<291::AID-IMMU291>3.0
更新日期:2002-01-01 00:00:00
abstract::High-dose infusion of IgG (IVIG) is used to treat autoimmune and inflammatory diseases, including Kawasaki disease (KD). Although the immunomodulatory effects of IVIG on blood cells such as macrophages have been well studied, its effects on tissue cells remain unclear. Here, we show that high-dose IgG specifically and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242398
更新日期:2012-08-01 00:00:00
abstract::To clarify reasons of the constant presence of IgM in human cord blood, some functions of this immunoglobulin class were analyzed. Cord IgM was collected from cord blood, and the cord IgM F(ab')2 fragment was obtained by trypsin cleavage. Maternal T and adult T cells were stained with fluorescein isothiocyanate (FITC)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110208
更新日期:1981-02-01 00:00:00
abstract::The regulation of nuclear factor (NF)-kappa B activation by the T cell receptor (TcR)/CD3 complex in primary human T cells has been studied at various times after activation. Only p50 NF-kappa B protein bound the kappa B element of interleukin-2 receptor (IL-2R) alpha chain promoter on resting T cells. However, immedi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250130
更新日期:1995-01-01 00:00:00
abstract::Cells in homeostasis metabolize glucose mainly through the tricarboxylic acid cycle and oxidative phosphorylation, while activated cells switch their basal metabolism to aerobic glycolysis. In this study, we examined whether metabolic reprogramming toward aerobic glycolysis is important for the host response to Mycoba...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546259
更新日期:2016-11-01 00:00:00
abstract::A panel of monoclonal antibodies (mAb) specific for the C gamma 2, C gamma 3 or inter C gamma 2/C gamma 3 domain epitopes was tested for inhibition of antibody-dependent cellular cytotoxicity (ADCC) specific for anti-D IgG-coated erythrocytes. Significant inhibition of ADCC was demonstrable for some antibodies having ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151015
更新日期:1985-10-01 00:00:00
abstract::Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunizati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050612
更新日期:1976-06-01 00:00:00
abstract::The forkhead-family transcription factor FoxP3 is important for the development and function of CD4+CD25+ regulatory T cells. While the overall phenotypic effects of FoxP3 expression are evident, the mechanism by which FoxP3 regulates T cell activation is not well understood. CD4+ T cells from mice that express a FoxP...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526339
更新日期:2005-12-01 00:00:00
abstract::Mice with homologous disruption of the gene coding for either the p35 subunit or the p40 subunit of interleukin-12 (IL-12) and derived from a strain genetically resistant to infection with Leishmania major have been used to study further the role of this cytokine in resistance to infection and the differentiation of f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260722
更新日期:1996-07-01 00:00:00
abstract::During the course of many human autoimmune diseases, antibodies which recognize negatively charged epitopes on self antigens are detected. Trypanosoma cruzi, an intracellular protozoan parasite capable of infecting a wide variety of vertebrates, is the cause of Chagas disease in humans. Infection with the parasite fre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221204
更新日期:1992-12-01 00:00:00
abstract::The F1 progeny of autoimmune NZB and normal SWR mice uniformly develop severe and accelerated lupus nephritis. The (SWR x NZB)F1 mice produce an oligoclonally expanded population of nephritogenic anti-DNA autoantibodies that share a recurrent cross-reactive idiotype (Id564), use highly homologous VH genes and surprisi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220302
更新日期:1992-03-01 00:00:00
abstract::The genetic control of in vitro primary syngeneic sensitization on monolayers of thyroid epithelial cells was investigated both at the level of the responder lymphocytes and that of stimulator thyroid epithelial cells using congenic resistant strains of mice. It clearly appears that similar to the in vivo model of exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131115
更新日期:1983-11-01 00:00:00
abstract::The expression of human leukocyte function-associated antigens (HLFA, equivalents of murine LFA-1) was studied on early lympho-hemopoietic cells in infant thymus and normal bone marrow by double immunofluorescence methods, cell sorting and colony-forming assays. Monoclonal antibodies MHM-24 recognizing the alpha chain...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160513
更新日期:1986-05-01 00:00:00
abstract::We identified the presence in the Aleutian skate, Bathyraja aleutica, of two classes of immunoglobulins (Ig), a high molecular weight Ig analogous to mammalian IgM and a low molecular weight Ig, similarly to the spiny rasp skate, Raja kenojei, (Kobayashi, K. et al., Mol. Immunol. 1984. 21: 397), using an immunological...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150916
更新日期:1985-09-01 00:00:00