Abstract:
:Rabbit antibodies to Atlantic cod (Gadus morhua L.) immunoglobulin were affinity purified and used to screen cDNA libraries from spleen and head kidney mRNA. cDNA clones for both the secretory and membrane-bound heavy (H) chain were isolated, the nucleotide and deduced amino acid sequences of which are reported here. Comparisons of the cod secretory H chain amino acid sequence show 24%, 27%, 30% identity to the mu chain of Mus, Xenopus and Ictalurus, respectively. The highest degree of identity was observed in the CH4 domain. The cDNA encoding the transmembrane form shows a novel splicing pattern where the TM1 exon is spliced directly onto the CH3 domain and not to the CH4 domain as in other animal groups. Southern blot analyses with VH and C probes on genomic DNA from cod erythrocytes indicate that there is a unique C gene but several V genes in the cod immunoglobulin H chain locus.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bengtén E,Leanderson T,Pilström Ldoi
10.1002/eji.1830211219subject
Has Abstractpub_date
1991-12-01 00:00:00pages
3027-33issue
12eissn
0014-2980issn
1521-4141journal_volume
21pub_type
杂志文章abstract::The MRC OX-22 monoclonal antibody recognizes a restricted determinant of the rat leukocyte-common antigen (L-CA, CD45), which is expressed on most peripheral T cells and all B cells. In contrast only 2%-3% of thymocytes are OX-22+ and these are now shown to be mostly of the immature CD4-CD8- (double-negative, DN), phe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191217
更新日期:1989-12-01 00:00:00
abstract::Using the patch-clamp technique in combination with fluorescence microscopy we have found an abnormality in voltage-gated K+ channel expression in T cells that represents the first molecular marker linking three disparate autoimmune diseases in mice. CD4-CD8-Thy-1.2+ (double-negative or DN) lymphocytes from every know...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200406
更新日期:1990-04-01 00:00:00
abstract::As shown recently, CD3+/TcR+ functional T lymphocytes can be derived in culture from embryonic liver cell precursors at a gestational age (6-8 weeks) preceding the colonization of the epithelial thymus. In this report, we analyzed the V beta repertoire of T lymphocytes derived from embryonic liver by applying a quanti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240949
更新日期:1994-09-01 00:00:00
abstract::Traumatic brain injury (TBI) elicits innate inflammatory responses that can lead to secondary brain injury. To better understand the mechanisms involved in TBI-induced inflammation, we examined the nature of macrophages responding to TBI in mice. In this model, brain macrophages were increased >20-fold the day after i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201243084
更新日期:2013-08-01 00:00:00
abstract::Recent studies have shown that in humans the germinal center reactions produce three types of V(D)J mutated B cells in similar proportions, i.e. Ig-switched, IgD-IgM+ (IgM-only) and IgD+IgM+ cells, and that together they form the CD27+ compartment of recirculating B cells. We investigated the Ig isotype switch capacit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200101)31:1<243::AID-IMMU243>3.0
更新日期:2001-01-01 00:00:00
abstract::Intravenous immunoglobulin G (IVIg) therapy is widely used to treat autoimmune and inflammatory diseases. Recent evidence suggests that in mice, splenic resident cells might be important for the anti-inflammatory activity of IVIg in a model of serum transfer arthritis. Splenectomized human immunothrombocytopenia (ITP)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142260
更新日期:2012-04-01 00:00:00
abstract::Normal human peripheral blood lymphocytes were shown to activate complement in normal human serum (NHS). This activation led to C3 fixation on the cell membrane, which in turn was visualized by fluorescence. The reaction occurred in the absence of detectable amounts of antibodies, since an agammaglobulinemic patient's...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090115
更新日期:1979-01-01 00:00:00
abstract::Tyrosine kinase 2 (Tyk2) contributes to the signals triggered by IL-12 for IFN-gamma production by NK cells and T cells. We found in this study that Tyk2-deficient (-/-) mice showed increased susceptibility at the early stage after an i.p. infection with Listeria monocytogenes, accompanied by impaired IFN-gamma produc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636173
更新日期:2006-11-01 00:00:00
abstract::Growing evidence suggests that some immune responses are mediated not only by conventional and distinct NK cells and CTL, but also by T cell subsets expressing NK receptors and NK cell-associated molecules. Consistent with our previously published finding that the mAb 1F8 identifies non-T/non-B cells in Xenopus that e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1574::AID-IMMU1574>3
更新日期:2002-06-01 00:00:00
abstract::Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a member of the immunoglobulin gene superfamily (IgSF) which is expressed by activated leukocytes and thymic epithelial cells and is a ligand for the lymphocyte antigen CD6. Herein, we report on the isolation and characterization of cDNA clones encoding mous...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270625
更新日期:1997-06-01 00:00:00
abstract::This study analyzes the role of the measles virus (MV) receptor, i.e. the human CD46 molecule, in the MHC class II-restricted presentation of MV hemagglutinin (H). We generated transgenic mice ubiquitously expressing CD46, with a similar level of transgene expression on the surface of antigen-presenting cells (APC), i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199804)28:04<1301::AID-IMM
更新日期:1998-04-01 00:00:00
abstract::The 95-kDa antigen recognized by the anti-CD19 panel of monoclonal antibodies is found on the surface of most cells of the B cell lineage. Anti-CD19 antibodies inhibit B cell proliferation in response to anti-Ig plus interleukin 4 (IL4), but enhance the response to mitogenic concentrations of either phorbol 12-myrista...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210302
更新日期:1991-03-01 00:00:00
abstract::A fraction of activated CD8+ T cells expresses CD40 ligand (CD40L), a molecule that plays a key role in T cell-dependent B cell stimulation. CD8+ T cell clones were examined for CD40L expression and for their capacity to allow the growth and differentiation of B cells, upon activation with immobilized anti-CD3. Accord...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251039
更新日期:1995-10-01 00:00:00
abstract::Lipopolysaccharide (LPS) is a potent inducer of interleukin 1 (IL 1) synthesis and release, and of tumor necrosis factor (TNF) secretion. Many signals can enhance the LPS-induced production of these cytokines. We have previously observed that addition of low amounts of normal human serum to the culture medium enhances...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200204
更新日期:1990-02-01 00:00:00
abstract::The existence of a functional receptor for secretory component (SC) on the eosinophil membrane might explain the preferential degranulation induced by secretory IgA (sIgA) when compared to serum IgA. Indeed, flow cytometry analysis revealed that purified human SC could bind to a subpopulation (4-59%) of blood eosinoph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250121
更新日期:1995-01-01 00:00:00
abstract::Ly49Q, a type II C-type lectin expressed on mouse plasmacytoid DC (pDC), contains a single carbohydrate recognition domain in its extracellular region and an ITIM in its cytoplasmic domain. We have identified the MHC class I molecule H-2K(b) as a Ly49Q ligand, confirming prior reports. Although H-2K(b) is expressed on...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838363
更新日期:2009-05-01 00:00:00
abstract::The CD4 T cell surface molecule binds MHC class II determinants expressed on antigen-presenting cells. CD4 is thought to enhance T cell activation by serving as an adhesion molecule as well as possibly by transducing an independent intracellular signal during the process of antigen stimulation. The recent observation ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200628
更新日期:1990-06-01 00:00:00
abstract::Genetic control has been studied of the response to the encephalitogenic nonapeptide (NP) determinant of myelin basic protein (BP) in inbred guinea pigs of strains resistant or susceptible to induction of experimental autoimmune encephalomyelitis (EAE). By studying bone marrow-reconstituted animals, we found that susc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110409
更新日期:1981-04-01 00:00:00
abstract::Human Vgamma9Vdelta2 T cells contribute to immunity against intracellular pathogens and recognize nonpeptidic antigens, such as the mycobacterial phosphoantigen TUBAg. HIV infection is associated with a polyclonal decrease of peripheral Vgamma9Vdelta2 T cells and we previously reported that the remaining cells show a ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199901)29:01<90::AID-IMMU9
更新日期:1999-01-01 00:00:00
abstract::Cross-linking of the high-affinity IgE receptor (FcepsilonRI) on mast cell activates signaling pathways that trigger degranulation and the release of multiple pro-inflammatory mediators. Mature,immature and precursor mast cells are degranulation competent. We show here that the signaling protein SWAP-70 has a function...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1121::AID-IMMU1121>3
更新日期:2002-04-01 00:00:00
abstract::T-cell destiny during thymic selection depends on the affinity of the TCR for autologous peptide ligands presented in the context of MHC molecules. This is a delicately balanced process; robust binding leads to negative selection, yet some affinity for the antigen complex is required for positive selection. All TCRs o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242606
更新日期:2012-12-01 00:00:00
abstract::The antigen receptor of the T lymphocytes is one of the most complex eukaryotic membrane structures studied to date. The T cell receptor (TcR) consists of two disulfide-linked glycoprotein chains (alpha/beta or gamma/delta) and is noncovalently associated with a group of small and invariable CD 3 proteins. Four CD 3 c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180508
更新日期:1988-05-01 00:00:00
abstract::The T cell repertoire of BALB/c mice contains clones capable of recognizing p-azobenzenearsonate (ABA)-tyrosine (Tyr) in association with both I-A and I-E-encoded class II molecules. Immunization of BALB/c animals with ABA-GAT (terpolymer of L-Glu60-L-Ala30-L-Tyr10) or ABA-GLT (terpolymer of L-Glu51-L-Lys34-L-Tyr15) i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151214
更新日期:1985-12-01 00:00:00
abstract::The Fas antigen (Fas), which is a cell surface protein belonging to the tumor necrosis factor receptor family, mediates apoptosis. To assess the contribution of Fas to the pathogenesis of retrovirus-induced immunodeficiency, we examined the kinetics of Fas expression on the lymphocytes during the course of murine acqu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241028
更新日期:1994-10-01 00:00:00
abstract::In this study, we examined which cell population contributes to IL-5 production by Peyer's patch (PP) cells. Thy1.2(-) fraction of PP cells, but not those of splenocytes, secreted IL-5 in response to IL-2. We found that CD3epsilon(-)IL-2Ralpha(+) cells purified from the Thy1.2(-)B220(-) fraction of PP cells secreted I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324696
更新日期:2004-07-01 00:00:00
abstract::Antigen-presenting macrophages (M phi) were derived from day 7 cultures of bone marrow stem cells using L cell conditioned medium. The adherent bone marrow-derived macrophages (BMM phi) were 100% esterase-positive, 95% positive for C3 receptors, 93% positive for Fc receptors, and 95% actively phagocytic. Indirect immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121202
更新日期:1982-12-01 00:00:00
abstract::DC-based vaccination against Leishmania major induces a parasite-specific Th1 response and long-lasting protective immunity in susceptible mice. Since distinct DC subsets have been proposed to direct the predominant development of either Th1 or Th2 cells, we analyzed the capability of plasmacytoid DC (pDC) to induce p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636780
更新日期:2007-09-01 00:00:00
abstract::Low zone tolerance (LZT) to contact allergens is induced by epicutaneous exposure to haptens in subsensitizing doses resulting in an inhibition of contact hypersensitivity (CHS), which, in contrast, occurs after sensitization with immunogenic doses of allergens. Performing the protocol of tolerance induction resulted ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425402
更新日期:2004-11-01 00:00:00
abstract::Expression of the complement-regulatory proteins (CRP) CD46, CD55 and CD59 represents a strategy used by tumor cells to evade complement-dependent cell cytoxicity stimulated by monoclonal antibodies. We have isolated two single-chain variable fragments (scFv) to CD55 and CD59 from a human phage-display library and fro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425920
更新日期:2005-07-01 00:00:00
abstract::Using immunoglobulin (Ig)-recognizing T helper clones the expression of Ig peptide/major histocompatibility complex class II complexes derived by the processing of endogeneous Ig molecules in the thymus was demonstrated. It was found that thymic B cells but not "classic" thymic antigen-presenting cells and macrophages...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201012
更新日期:1990-10-01 00:00:00