Autoimmune diseases linked to abnormal K+ channel expression in double-negative CD4-CD8- T cells.

Abstract:

:Using the patch-clamp technique in combination with fluorescence microscopy we have found an abnormality in voltage-gated K+ channel expression in T cells that represents the first molecular marker linking three disparate autoimmune diseases in mice. CD4-CD8-Thy-1.2+ (double-negative or DN) lymphocytes from every known murine model for systemic lupus erythematosus, type-1 diabetes mellitus and experimental allergic encephalomyelitis exhibit abnormally high numbers of an unusual K+ channel, termed type l compared to their phenotypic counterparts in normal mice. Other T cell subsets from these diseased mice retain their normal pattern of K+ channel expression. The unique K+ channel phenotype of DN T cells arises in parallel with the onset of autoimmunity. Although mitogen-activated T cells and rapidly proliferating thymocytes exhibit large numbers of K+ channels, these channels are of an electrophysiologically distinct type called n. Thus, abundant expression of type l K+ channels appears to be a useful marker for DN T cells associated with autoimmunity and may provide a valuable tool for delineating the role of DN T cells in the pathogenesis of autoimmune diseases.

journal_name

Eur J Immunol

authors

Chandy KG,Cahalan MD,Grissmer S

doi

10.1002/eji.1830200406

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

747-51

issue

4

eissn

0014-2980

issn

1521-4141

journal_volume

20

pub_type

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