Abstract:
:Normal human peripheral blood lymphocytes were shown to activate complement in normal human serum (NHS). This activation led to C3 fixation on the cell membrane, which in turn was visualized by fluorescence. The reaction occurred in the absence of detectable amounts of antibodies, since an agammaglobulinemic patient's serum also supported complement activation, and the results were unaltered by absorption of normal human serum. Trypsin treatment of lymphocytes to remove possible complement activators bound to the cell membrane, did not have any effect on the complement activation by lymphocytes. Complement membrane fluorescence was abolished in the presence of EDTA or ethyleneglycolbis(aminoethylether) tetraacetate (EGTA) supplemented by Mg++ions indicating that the classical pathway of complement activation was involved in the reaction. Experiments performed with T and B-purified populations showed that the phenomenon was confined to the B cell fraction. Complement deposition did not diminish the number of EAC rosettes, and viability remained unchanged after exposure of lymphocytes to normal human serum.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Gutiérrez C,Vega J,Kreisler Mdoi
10.1002/eji.1830090115subject
Has Abstractpub_date
1979-01-01 00:00:00pages
72-6issue
1eissn
0014-2980issn
1521-4141journal_volume
9pub_type
杂志文章abstract::The second HLA-DR molecules, which are encoded by loci different from HLA-DRB1 are weakly polymorphic. Predominant alleles such as HLA-DRB3*0101, HLA-DRB4*0101 and HLA-DRB5*0101 are therefore interesting targets to define antigenic peptides with major impact for the entire population. Strikingly, they have been poorly...
journal_title:European journal of immunology
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abstract::Cellular metabolism is dynamically regulated in NK cells and strongly influences their responses. Metabolic dysfunction is linked to defective NK cell responses in diseases such as obesity and cancer. The transcription factors, sterol regulatory element binding protein (SREBP) and cMyc, are crucial for controlling NK ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048784
更新日期:2020-09-18 00:00:00
abstract::The potential of synthetic peptides as vaccines is restricted by their frequent lack of immunogenicity. As with haptens, coupling to a carrier protein is usually required to provide T cell help to anti-peptide antibody-producing B cells. In spite of their short length, a few natural or synthetic peptides are immunogen...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150823
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:2018-12-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1977-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
abstract::Strains CBA (M523) (= M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B 10.D2 (M504 (= M504) and b 10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CB...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090208
更新日期:1979-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242557
更新日期:2013-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271004
更新日期:1997-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242974
更新日期:2013-02-01 00:00:00
abstract::Lymphocyte infiltration to pancreatic islets is associated to chemoattraction, as are other inflammatory autoimmune processes. We examined whether development of insulitis and diabetes depends on chemoattraction of lymphocytes via the CCR5 chemokine receptor. In non-obese diabetic (NOD) mice, a substantial fraction of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324285
更新日期:2004-02-01 00:00:00
abstract::The murine X-linked lymphoproliferative disease scurfy is similar to the Wiskott-Aldrich syndrome in humans. Disease in scurfy (sf) mice is mediated by CD4+ T cells. Based on similarities in scurfy mice and transgenic mice that overexpress specific cytokine genes, we evaluated the expression of cytokines in the lesion...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260125
更新日期:1996-01-01 00:00:00
abstract::Leptin, a protein produced by adipocytes, exerts several functions, including modulation of the immune response. A report in this issue of the European Journal of Immunology describes for the first time the effect of either leptin receptor deficiency or blockade on murine dendritic cell (DC) maturation, survival and f...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2006-12-01 00:00:00
abstract::The cell surface expression and biosynthesis of Langerhans cells (LC)-derived major histocompatibility complex (MHC) class II molecules from epidermal cells (EC) prepared freshly and cultured for up to 3 days was investigated. Based on the constitutive expression of MHC class II determinants by LC, a panning and magne...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2014-12-01 00:00:00
abstract::The initiation of CD8(+) T cell (CTL) immune responses can occur via cross-priming. Recent data suggested a relationship between cross-presentation and immunodominance of epitope-specific T cells. To test this association, we evaluated the efficacy of cross-presentation for several virus epitopes in vitro and examined...
journal_title:European journal of immunology
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更新日期:2010-08-01 00:00:00
abstract::To clarify reasons of the constant presence of IgM in human cord blood, some functions of this immunoglobulin class were analyzed. Cord IgM was collected from cord blood, and the cord IgM F(ab')2 fragment was obtained by trypsin cleavage. Maternal T and adult T cells were stained with fluorescein isothiocyanate (FITC)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110208
更新日期:1981-02-01 00:00:00
abstract::Hypersensitivity to nickel (Ni) represents the most common manifestation of contact allergy in humans. The role of metal-specific T cells in this disease is well established, but the molecular interactions involved in their activation are poorly understood. We examined the T cell receptor (TCR) repertoire in T cells a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270808
更新日期:1997-08-01 00:00:00
abstract::Human T cell clones expressing the T cell receptor (TcR) gamma/delta were isolated from peripheral blood lymphocytes of two unrelated donors. The TcR gamma/delta+ clones derived from one of these donors were all of the Ti gamma A+, delta-TCS1-, BB3+ phenotype indicating the exclusive use of the V gamma 9 and V delta 3...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191120
更新日期:1989-11-01 00:00:00
abstract::Activation of T cells was shown to up-regulate the Fas ligand (FasL) which binds to the CD95 (APO-1/Fas) antigen and mediates activation-induced cell death (AICD) of activated T cells and T lymphoma cells. A recent report showed that mouse B cells express the FasL upon activation with lipopolysaccharide (LPS). We ther...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270433
更新日期:1997-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200211)32:11<3152::AID-IMMU3152>
更新日期:2002-11-01 00:00:00
abstract::A novel surface molecule, Tp90, is described which appears to be involved in an antigen-independent pathway of human T lymphocyte activation. The Tp90 molecule was identified by a monoclonal antibody (mAb), MX20, obtained from a fusion using spleen cells of a mouse immunized with cells from two T cell leukemia lines, ...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
abstract::To study the biological consequences of cytokine production and apoptosis by intraepithelial lymphocytes (IEL), we have studied these characteristics in both the high and low density CD3+ IEL populations. Stimulation of low- or high-density CD3+ IEL via the T cell receptor (TCR)-CD3 complex using monoclonal anti-CD3, ...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00