Abstract:
:To clarify reasons of the constant presence of IgM in human cord blood, some functions of this immunoglobulin class were analyzed. Cord IgM was collected from cord blood, and the cord IgM F(ab')2 fragment was obtained by trypsin cleavage. Maternal T and adult T cells were stained with fluorescein isothiocyanate (FITC)-conjugated cord IgM and FITC-cord F(ab')2 mu. Cord IgM, unlike cord IgG, bound to about one-third of maternal and one-fourth of adult T cells and killed them, but it killed only a small proportion of the B cells. However, adult F(ab)'2 mu did not kill these cells. Cord T cells were not killed by cord IgM. Thus, it is apparent that cord IgM contains cytotoxic antibodies against maternal and adult T cells. T lymphocytotoxic fetal antibody (TLFA) may be largely responsible for the absence of maternal T cells in cord blood. The percentage of [3H]thymidine incorporation into concanavalin A-induced adult mononuclear cells treated with cord IgM containing TLFA was 56.0 +/- 17.9% (p less than 0.01) and that into pokeweed mitogen-induced cells treated with the cord IgM preparation was 100.3 +/- 17.8% (p greater than 0.05) indicating the possibility that TLFA might kill a certain subset(s) of T cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Miyagawa Y,Komiyama A,Akabane Tdoi
10.1002/eji.1830110208subject
Has Abstractpub_date
1981-02-01 00:00:00pages
106-9issue
2eissn
0014-2980issn
1521-4141journal_volume
11pub_type
杂志文章abstract::Lymphoid follicles of the ileal Peyer's patch (PP) of young sheep function as the major source of B cells and a site of immunoglobulin (Ig) receptor diversification. However, extensive cell death in culture has restricted investigations of ileal PP follicular (iPf)B cell biology. We investigated the possibility that s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240220
更新日期:1994-02-01 00:00:00
abstract::Soluble anti-immunoglobulin (Ig) antibodies have been generally found to inhibit Ig secretion in B cells, via largely unknown mechanisms. To investigate this phenomenon further a two-step culture system was used in which B cells are primed for 24-72 h with various soluble monoclonal or polyclonal anti-Ig antibodies: a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220629
更新日期:1992-06-01 00:00:00
abstract::B cell development in organ cultures of fetal liver from mice at day 14 of gestation resembles in kinetics and cell numbers generated the one observed in vivo. This development in vitro can be blocked by an IL-7 receptor-specific monoclonal antibody. Monoclonal antibodies specific for the pre-B cell receptor, i. e. fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200003)30:3<759::AID-IMMU759>3.0
更新日期:2000-03-01 00:00:00
abstract::Genetic control has been studied of the response to the encephalitogenic nonapeptide (NP) determinant of myelin basic protein (BP) in inbred guinea pigs of strains resistant or susceptible to induction of experimental autoimmune encephalomyelitis (EAE). By studying bone marrow-reconstituted animals, we found that susc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110409
更新日期:1981-04-01 00:00:00
abstract::IL-10 is a potent anti-inflammatory molecule, which regulates TNF-alpha at multiple levels. We investigated whether IL-10 also modulated the activity of the TNF-alpha-converting enzyme (TACE). Using an ex vivo fluorogenic assay we observed that LPS rapidly induced TACE activity in monocytes coinciding with release of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737821
更新日期:2008-04-01 00:00:00
abstract::Lymphocyte function-associated-antigen-1 (LFA-1) is able to bind selectively to its ligands intercellular adhesion molecules 1 and 3 (ICAM-1 and ICAM-3), suggesting that LFA-1 can exist in distinct ligand-specific binding states. In the case of ICAM-1, apart from ligand itself and the recently cloned molecule cytohesi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270423
更新日期:1997-04-01 00:00:00
abstract::Human T cell clones expressing the T cell receptor (TcR) gamma/delta were isolated from peripheral blood lymphocytes of two unrelated donors. The TcR gamma/delta+ clones derived from one of these donors were all of the Ti gamma A+, delta-TCS1-, BB3+ phenotype indicating the exclusive use of the V gamma 9 and V delta 3...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191120
更新日期:1989-11-01 00:00:00
abstract::Rabbit antibodies to Atlantic cod (Gadus morhua L.) immunoglobulin were affinity purified and used to screen cDNA libraries from spleen and head kidney mRNA. cDNA clones for both the secretory and membrane-bound heavy (H) chain were isolated, the nucleotide and deduced amino acid sequences of which are reported here. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211219
更新日期:1991-12-01 00:00:00
abstract::Proliferation of BCL1 cells, generally used for the measurement of murine interleukin (IL)5, is shown to be strongly suppressed by interferon (IFN)-gamma. This effect can be abrogated by the addition of anti-IFN-gamma antibodies. Thus, the IL5 activity of culture supernatants derived from T cells simultaneously produc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190726
更新日期:1989-07-01 00:00:00
abstract::Human natural killer (NK) cells can be subdivided into two populations based on the density of cell surface CD56 antigen. The great majority (approximately 90%) of NK cells express CD56 at low levels (the CD56dim phenotype), whereas a small NK cell subset (approximately 10%) exhibits approximately fivefold greater den...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220102
更新日期:1992-01-01 00:00:00
abstract::Plasticity and heterogeneity are hallmarks of myelomonocytic differentiation and polarized activation. Evidence published in this issue of the European Journal of Immunology, together with other recent data, add new elements and perspectives to the current understanding of mononuclear phagocyte differentiation and act...
journal_title:European journal of immunology
pub_type: 评论,杂志文章,评审
doi:10.1002/eji.200636910
更新日期:2007-01-01 00:00:00
abstract::Despite accounting for only a minor fraction of all cells in Hodgkin's lymphoma tissue, the Hodgkin and Reed-Sternberg (HRS) cells represent the malignant tumor cell clone in Hodgkin's disease (HD). By far the most abundant cell type in the tumor tissue are CD4+ T cells. Some of them intimately associate with HRS cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199808)28:08<2424::AID-IMM
更新日期:1998-08-01 00:00:00
abstract::A simple and efficient procedure for the construction of bifunctional molecules is described and their use in a variety of applications documented. This procedure is based on our observation that mouse IgG1 monoclonal antibodies, when mixed with equimolar amounts of a high-affinity rat monoclonal antibody specific for...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160615
更新日期:1986-06-01 00:00:00
abstract::T lymphocyte subsets expressing either T cell receptor alpha/beta or gamma/delta were selected from human peripheral blood T cells and proliferative responses to molecular mass-fractionated mycobacterial lysates were determined. alpha/beta T cells primarily responded to fractions greater than 30 kDa whereas gamma/delt...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200534
更新日期:1990-05-01 00:00:00
abstract::Type I IFN play a very important role in immunity against viral infections. Murine type I IFN belongs to a multigene family including 14 IFN-alpha subtypes but the biological functions of IFN-alpha subtypes in retroviral infections are unknown. We have used the Friend retrovirus model to determine the anti-viral effec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838311
更新日期:2009-01-01 00:00:00
abstract::Immunodominant T cell epitopes from the autoantigen human cartilage glycoprotein 39 have previously been mapped in the context of HLA-DR*0401 and *0402, using mice expressing HLA-DR4 transgenes. We measured the dissociation rates of these epitopes from soluble recombinant DR*0401 and DR*0402 to assess the relationship...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200203)32:3<662::AID-IMMU662>3.0
更新日期:2002-03-01 00:00:00
abstract::CD40 ligand (CD40L) is a 33-kDa type II membrane glycoprotein induced on T cells upon activation. CD40L has previously been shown to induce proliferation of resting B cells, immunoglobulin (Ig) secretion from B cells cultured with cytokines and cytokine secretion and tumoricidal activity from monocytes. In this report...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230941
更新日期:1993-09-01 00:00:00
abstract::T cell activation and cytokine secretion are important mediators of inflammation in allergic asthma. The costimulatory pathway CD28/CD80/CD86 has been shown to play an important role in T cell activation in allergic asthma, but less is known about the effect of other costimulatory molecules in allergy. The costimulato...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2002010)32:10<2874::AID-IMMU2874
更新日期:2002-10-01 00:00:00
abstract::Recent studies have suggested that T cell memory for recall antigens resides in clones of primed T cells with a short inter-mitotic half-life. In humans such cells express an isoform of the leukocyte common antigen termed CD45RO. Nevertheless, little is known of the fate of these primed T cells after initial activatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240417
更新日期:1994-04-01 00:00:00
abstract::Th17 cells are defined by their capacity to produce IL-17, and are important mediators of inflammation and autoimmunity. Human Th17 cells express high levels of the retinoic acid-related orphan receptor variant 2 (RORC2), but it is currently unclear whether expression of this transcription factor alone is sufficient t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838908
更新日期:2009-06-01 00:00:00
abstract::The expression and action of Fas/Fas ligand (FasL) in multiple sclerosis has been postulated as a major pathway leading to inflammatory demyelination. To formally test this hypothesis, C57BL/6-lpr and -gld mice, which due to gene mutation express Fas and FasL in an inactive form, were immunized with myelin oligodendro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271211
更新日期:1997-12-01 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma (PPARgamma ), a member of the nuclear receptor superfamily, has recently been described as a modulator of macrophage functions and as an inhibitor of T cell proliferation. Here, we investigated the role of PPARgamma in dendritic cells (DC), the most potent antigen-prese...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2001010)31:10<2857::aid-immu2857
更新日期:2001-10-01 00:00:00
abstract::Low zone tolerance (LZT) to contact allergens is induced by epicutaneous exposure to haptens in subsensitizing doses resulting in an inhibition of contact hypersensitivity (CHS), which, in contrast, occurs after sensitization with immunogenic doses of allergens. Performing the protocol of tolerance induction resulted ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425402
更新日期:2004-11-01 00:00:00
abstract::We have previously reported the development of a two-step culture system in which soluble anti-mu monoclonal antibodies prime small resting murine B cells to secrete immunoglobulin (Ig) in response to restimulation with a mixture of interleukin-4 (IL-4) and IL-5. Here we have extended these studies to investigate the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230243
更新日期:1993-02-01 00:00:00
abstract::T cell stimulation of B cell proliferation during T-B collaboration requires membrane-bound stimulatory ligands, such as CD40 ligand and the secretion of soluble cytokines, such as IL-4. Nevertheless, it remains unclear whether T cell contact is required to provoke each consecutive B cell division, or whether B cells ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200104)31:4<1150::aid-immu1150>3
更新日期:2001-04-01 00:00:00
abstract::Polymorphonuclear neutrophils (PMN) modulate the adaptive immune response through interactions with immature dendritic cells (iDC) while spontaneous apoptotic neutrophils PMNapo (PMNapo) may have an inhibitory effect on DC functions. We investigate the effect exerted by PMNapo in DC maturation and the role of Mycobact...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636771
更新日期:2007-06-01 00:00:00
abstract::Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilized multip...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847511
更新日期:2018-09-01 00:00:00
abstract::Cells of the innate immune system build immunological memory via epigenetic reprogramming after stimulations with microbial ligands. This functional readjustment allows for enhanced nonspecific inflammatory responses upon secondary challenges, a process termed "trained immunity." The epigenomic blueprint of trained mo...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201545497
更新日期:2016-04-01 00:00:00
abstract::NK cells and cytotoxic T lymphocytes can induce apoptosis in virus-infected and transformed target cells via the granule exocytosis pathway. The key components of the cytolytic granules are perforin and several serine esterases, termed granzymes. While the cellular distribution of human granzymes A (GrA) and B (GrB) h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526122
更新日期:2005-09-01 00:00:00
abstract::Vaccine efficacy largely depends upon DC targeting and activation. The most potent TLR soluble ligands induce diffuse DC activation, which may be associated with marked pro-inflammatory responses and possibly adverse effects. This raises the concern that effective vaccine adjuvants may similarly rely on widespread DC ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737889
更新日期:2008-05-01 00:00:00