Abstract:
:Vaccine efficacy largely depends upon DC targeting and activation. The most potent TLR soluble ligands induce diffuse DC activation, which may be associated with marked pro-inflammatory responses and possibly adverse effects. This raises the concern that effective vaccine adjuvants may similarly rely on widespread DC activation. Using a promising candidate vaccine against tuberculosis (fusion protein of Ag85B and 6-kDa early secretory antigenic target (ESAT-6)) formulated in the potent IC31 adjuvant, DC targeting and activation was studied in vivo, following the fate of antigen and adjuvant in the draining lymph nodes, to define the magnitude of DC targeting/activation required in vivo to induce protective vaccine responses. Unexpectedly, protective IFN-gamma-mediated Ag85B-ESAT-6/IC31 responses were associated to the activation of a minute population (less than 0.3%) of CD11c(+) lymph node DC, without detectable systemic pro-inflammatory responses. This activated peripheral tissue-derived DC population, characterized by enhanced CD80, CD86, CD40 and IL-12p40 expression, was only identified when focusing on adjuvant- or antigen-labeled CD11c(+) DC, which were found to support T cell proliferation. Immunization with aluminum hydroxide adjuvant (Alum) resulted in a similar proportion of antigen-associated DC but without detectable enhancement of CD80, CD86, CD40 or IL-12p40 expression. Thus, potent protective IFN-gamma-producing responses may be elicited by the exquisite activation of a minute number of in vivo targeted DC.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Kamath AT,Valenti MP,Rochat AF,Agger EM,Lingnau K,von Gabain A,Andersen P,Lambert PH,Siegrist CAdoi
10.1002/eji.200737889subject
Has Abstractpub_date
2008-05-01 00:00:00pages
1247-56issue
5eissn
0014-2980issn
1521-4141journal_volume
38pub_type
杂志文章abstract::Accumulating evidence suggests that Th17 cells and Tregs may exhibit development plasticity and that CD4(+) Tregs can differentiate into IL-17-producing T cells; however, whether Th17 cells can reciprocally convert into Tregs has not been described. In this study, we generated Th17 clones from tumor-infiltrating T lym...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040682
更新日期:2011-04-01 00:00:00
abstract::Injection of incomplete Freund's adjuvant (IFA) into the footpads of BALB/c mice induced an acute inflammation. Draining popliteal lymph nodes showed major histocompatibility complex (MHC) class II-restricted proliferation when challenged in vitro with recombinant Mycobacterium bovis 65-kDa heat shock protein (hsp65)....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230107
更新日期:1993-01-01 00:00:00
abstract::Exosomes are nanovesicles produced constitutively and inducibly by several types of cells. They are generated as intraluminal vesicles of multivesicular bodies and express MHC and several endosomal/lysosomal proteins. In spite of their potential role in cellular immunity, the regulatory mechanisms of exosome productio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737694
更新日期:2008-05-01 00:00:00
abstract::After incorporation of 14C-labeled amino acids and/or of [3H]fucose during in vitro culture of the Thy-1.1-bearing, Fc receptor-positive T lymphoma, L-5187-Y, attempts were made to purify the Fc binding structure(s). Following solubilization of a crude membrane pellet using sodium deoxycholate, the 110 000 x g superna...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070916
更新日期:1977-09-01 00:00:00
abstract::Interleukin-5 (IL-5) plays a critical role in the pathogenesis of eosinophil-associated allergic disorders, such as asthma. IL-5 may also play a major role in the development of eosinophilia-associated lymphoproliferative disorders caused by human T lymphotropic virus type I (HTLV-I). In this study, we have investigat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3694::aid-immu3694>
更新日期:2001-12-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease inducible in rodents by immunization of brain-specific antigens such as myelin basic protein (MBP). It is also well known that various strains of rats differ in their susceptibility to EAE upon active immunization. To elucidate the...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200934
更新日期:1990-09-01 00:00:00
abstract::Germinal center (GC) B cells undergo proliferation, somatic hypermutation and isotype switching in the course of differentiation into plasma cells to produce high-affinity antibodies. To understand the molecular mechanism regulating the expansion of memory B cells and the termination of expansion by differentiation in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199802)28:02<508::AID-IMMU
更新日期:1998-02-01 00:00:00
abstract::We have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231122
更新日期:1993-11-01 00:00:00
abstract::The thymus is the primary organ for T-cell differentiation and maturation. Unlike other major organs, the thymus is highly dynamic, capable of undergoing multiple rounds of almost complete atrophy followed by rapid restoration. The process of thymic atrophy, or involution, results in decreased thymopoiesis and emigrat...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201142305
更新日期:2012-05-01 00:00:00
abstract::Physical exercise, mental stress, or infusion of beta-adrenergic agonists result in an increase in the number of natural killer (NK) cells in the peripheral circulation. In view of the specific migration pattern of NK cells in vivo, it has been suggested that these cells may be released from the marginating pool in bl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231230
更新日期:1993-12-01 00:00:00
abstract::An experimental model of adenosine deaminase deficiency was established on the human T cell line Jurkat by using 2'-deoxycoformycin, a strong specific inhibitor of the enzyme. When deoxyadenosine was added to the inhibited cells, the nucleotide profile was modified reproducing that found in lymphocytes from adenosine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200322
更新日期:1990-03-01 00:00:00
abstract::The repertoire of V regions of alpha/beta+ T cell receptor (TcR) on CD4+ and CD8+ T cells from the peripheral blood of six patients with a novel X-linked combined immunodeficiency was investigated by flow cytometry. The relative frequencies of V region subfamilies V alpha 2a on CD4+ T lymphocytes and V beta 5b and V b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220435
更新日期:1992-04-01 00:00:00
abstract::The studies described in this report were designed to investigate factors that could influence the behavior of erythrocytes following their interaction with monoclonal antibodies (mAb) in a fully homologous experimental opsonization system in vivo. The clearance profiles and tissue distribution of target erythrocytes ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210413
更新日期:1991-04-01 00:00:00
abstract::Activated monocyte-macrophages have been implicated in tumor angiogenesis via their capacity to produce many potent angiogenic factors. However, the mechanisms leading to production of these angiogenic factors in macrophages remain to be elucidated. In this study, we demonstrated by use of a mouse Matrigel implantatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424957
更新日期:2004-08-01 00:00:00
abstract::A cDNA encoding the human beta Fc gamma receptor II (FcRII) was isolated from a placental cDNA library. Analysis of the predicted amino acid sequence indicates that this receptor is synthesized with a 42-amino acid leader sequence. The mature protein consists of 249 amino acids. The leader sequence and the cytoplasmic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200624
更新日期:1990-06-01 00:00:00
abstract::In this study the genetic control of major histocompatibility complex (MHC) class II gene expression during the transition from B cell to plasma cell has been analyzed. Class II molecules are not expressed in plasma cells because of an active suppression resulting in the abrogation of class II gene transcription. We s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261028
更新日期:1996-10-01 00:00:00
abstract::The CD4 and CD8 coreceptor molecules on immature thymocytes participate in T cell repertoire selection. To examine more definitively the role of CD4 and CD8 in the negative and positive selection of immature thymocytes, we generated transgenic mice with elevated surface CD4 expression and mated them with mice expressi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220317
更新日期:1992-03-01 00:00:00
abstract::We recently reported that parenteral injection of malaria palmitoyl-tailed peptides without adjuvant efficiently induces B, Th and CTL responses. We now show that intranasal (IN) or sub-lingual (SL) delivery of such lipopeptides induces strong systemic immune responses, as demonstrated by specific Th cell responses fr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200208)32:8<2274::AID-IMMU2274>3
更新日期:2002-08-01 00:00:00
abstract::A mouse T cell hybrid specific for pigeon cytochrome c in the context of I-Ek responds by secreting interleukin 2 when co-cultured with the native antigen and the B cell lymphoma, LK-35.2, or naive splenic B cells as antigen-presenting cells (APC). Cytochromes c and their corresponding C-terminal fragments which are n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160702
更新日期:1986-07-01 00:00:00
abstract::AMPK (adenosine monophosphate-activated protein kinase) is phosphorylated (AMPK-P) in response to low energy through allosteric activation by Adenosine mono- or diphosphate (AMP/ADP). Folliculin (FLCN) and the FLCN-interacting proteins 1 and 2 (FNIP1, 2) modulate AMPK. FNIP1 deficiency patients have a AMPK-P gain of f...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201948504
更新日期:2020-07-01 00:00:00
abstract::The transcriptional repressor Gfi1 is a nuclear zinc-finger protein that is expressed in T cell precursors in the thymus, but is down-regulated in mature, resting T cells. Gfi1 expression rises transiently to levels seen in thymocytes upon antigenic activation. We show here that lack of Gfi1 causes delayed cell cycle ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737130
更新日期:2007-12-01 00:00:00
abstract::Missing-self-reactivity can be mimicked by blocking self-specific inhibitory receptors on NK cells, leading to increased rejection of syngeneic tumor cells. Using a mouse model, we investigated whether Ab-mediated blocking of inhibitory receptors, to a degree where NK cells rejected syngeneic tumor cells, would still ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939755
更新日期:2010-03-01 00:00:00
abstract::Mitochondria are master regulators of metabolism. Mitochondria generate ATP by oxidative phosphorylation using pyruvate (derived from glucose and glycolysis) and fatty acids (FAs), both of which are oxidized in the Krebs cycle, as fuel sources. Mitochondria are also an important source of reactive oxygen species (ROS)...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201445427
更新日期:2016-01-01 00:00:00
abstract::gp140 is the Epstein-Barr virus receptor and the C3d receptor (EBVR/C3dR) of human B lymphocytes. Recently, we have shown that cross-linking of EBVR/C3dR on cell surface by polyclonal anti-gp140 induced B cell activation, in presence of T cell factors. Immunoregulatory abnormalities of EBV-induced B cell activation ha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161108
更新日期:1986-11-01 00:00:00
abstract::Monoclonal gammopathies (MG) are monoclonal proliferative disorders of B cells at the differentiation stage of Ig production. They can be detected in the serum, either as transient or as persistent homogenous immunoglobulin (H-Ig) components. The exact phenotype, localization, and cell lineage origin of the precursor ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270943
更新日期:1997-09-01 00:00:00
abstract::LL-37, derived from human cathelicidin, stimulates immune responses in neutrophils. Although FPR2 and P2X7 were proposed as LL-37 receptors, we have shown that among 21 neutrophil receptors only CXCR2 was down-regulated by LL-37. LL-37 functions similarly to CXCR2-specific chemokines CXCL1 and CXCL7 in terms of recept...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939496
更新日期:2009-11-01 00:00:00
abstract::This study reports on the earliest stages of mononuclear phagocyte differentiation. A crucial question in this developmental process is whether mature macrophage (M phi) heterogeneity is already appointed at the precursor cell level. For this purpose, we produced clonal populations of mononuclear phagocytes from bone ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200104
更新日期:1990-01-01 00:00:00
abstract::It is widely accepted that females have superior immune responses than males, but the ways by which sex hormones may enhance T cell responses are still poorly understood. In the present study, we analyzed the effect of estrogens on CD4 T cell activation and differentiation after immunization with exogenous antigens. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200310027
更新日期:2003-02-01 00:00:00
abstract::We analyzed the stimulating capacities of malignant B cells from non-Hodgkin's lymphomas (NHL) to induce an allogeneic response in primary mixed lymphocyte reaction (MLR). T cells purified from a single healthy donor (KS) were used to compare the responses induced by either malignant or hyperplastic cells. Malignant B...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251220
更新日期:1995-12-01 00:00:00
abstract::The development of preventive or therapeutic recombinant vaccines and the generation of serodiagnostic assays for infectious diseases depend essentially on the availability of molecularly defined antigens. A major bottleneck for the identification of suitable target antigens for many pathogens is the isolation of suff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535538
更新日期:2006-04-01 00:00:00