Abstract:
:The CD4 and CD8 coreceptor molecules on immature thymocytes participate in T cell repertoire selection. To examine more definitively the role of CD4 and CD8 in the negative and positive selection of immature thymocytes, we generated transgenic mice with elevated surface CD4 expression and mated them with mice expressing a transgenic T cell receptor. Augmented CD4 expression was found to markedly alter CD8-dependent negative and positive selection of T cells specific for the male (H-Y) antigen presented by H-2Db major histocompatibility complex class I molecules. Moreover, the cytoplasmic tail of CD4 was essential for effecting these alterations, since the overexpression of tailless CD4 molecules failed to influence the outcome of CD8-dependent selection. The inhibition of positive and negative selection in double-transgenic mice expressing the full-length CD4 molecule was associated with a decreased interaction between the protein tyrosine kinase p56lck and CD8. These results strongly implicate p56lck in T cell repertoire selection.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Van Oers NS,Garvin AM,Davis CB,Forbush KA,Carlow DA,Littman DR,Perlmutter RM,Teh HSdoi
10.1002/eji.1830220317keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
735-43issue
3eissn
0014-2980issn
1521-4141journal_volume
22pub_type
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