Abstract:
:Activated monocyte-macrophages have been implicated in tumor angiogenesis via their capacity to produce many potent angiogenic factors. However, the mechanisms leading to production of these angiogenic factors in macrophages remain to be elucidated. In this study, we demonstrated by use of a mouse Matrigel implantation model that mouse peritoneal macrophages induce angiogenesis. mRNA expression and protein synthesis of macrophage-derived crucial angiogenic factors such as IL-1, TNF-alpha, basic fibroblast growth factor, and vascular endothelial growth factor (VEGF) were blocked by platelet-activating factor (PAF) receptor antagonists. It was also observed that inhibitors of NF-kappaB blocked macrophage production of these angiogenic factors. Gene expression and protein synthesis of the angiogenic factors cited above were also inhibited in IkappaBalpha-mutated macrophages. VEGF is the most potent angiogenic factor in macrophage-induced angiogenesis. PAF antagonists or NF-kappaB inhibitors also inhibit the capacity of conditioned medium from LPS-stimulated human peripheral blood monocytes to induce sprouting of porcine pulmonary arterial endothelial cells. These data indicate that PAF-induced NF-kappaB activation is a common upstream pathway leading to the production of crucial macrophage-derived angiogenic factors. This will provide an important clue for a better understanding of mechanisms involved in tumor angiogenesis.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Seo KH,Ko HM,Choi JH,Jung HH,Chun YH,Choi IW,Lee HK,Im SYdoi
10.1002/eji.200424957keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
2129-37issue
8eissn
0014-2980issn
1521-4141journal_volume
34pub_type
杂志文章abstract::To define activation-specific sequences in human T lymphocytes, a cDNA library was constructed by subtractive hybridization using resting and stimulated peripheral blood lymphocyte pairs. Stimulation of peripheral blood lymphocytes was achieved by triggering with mitogenic anti-CD2 (T11) monoclonal antibodies. Differe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190614
更新日期:1989-06-01 00:00:00
abstract::Antibodies to framework determinants of the VH and V lambda fragments of MOPC 315 and antisera to the VH idiotype determinants of the A 5 A antibody were used to analyze the antigen receptors of mouse T (and B) cells. This was done by using the antibodies as inhibitors in (a) an assay in which the binding of radiolabe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100207
更新日期:1980-02-01 00:00:00
abstract::Activation of mature CD8+ T cells requires recognition, via the T cell receptor (TCR), of peptide + MHC (pMHC) complexes with an avidity that exceeds a designated threshold. Multiple indicators of T cell avidity have been described that provide unique information on the characteristics of T cell interactions. However,...
journal_title:European journal of immunology
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doi:10.1002/eji.200636390
更新日期:2006-11-01 00:00:00
abstract::This article describes the study of the functional relationship between auto-tumor-reactive CD4(+) T cell clones (TCC) and autologous malignant B cells. Four auto-tumor-reactive CD4(+) TCC were derived from tumor-infiltrating T lymphocytes (TIL-T) from a freshly isolated human follicular lymphoma by the following tech...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3188::AID-IMM
更新日期:1999-10-01 00:00:00
abstract::Using a specific antibody, we found that expression of the viral restriction factor IFITM3 differs across cell types within the immune compartment with higher expression in myeloid rather than lymphoid cells. IFITM3 expression was increased following IFN stimulation, mostly type I, in immune cells, with the exception ...
journal_title:European journal of immunology
pub_type: 信件
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更新日期:2020-10-30 00:00:00
abstract::Growing evidence suggests that some immune responses are mediated not only by conventional and distinct NK cells and CTL, but also by T cell subsets expressing NK receptors and NK cell-associated molecules. Consistent with our previously published finding that the mAb 1F8 identifies non-T/non-B cells in Xenopus that e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1574::AID-IMMU1574>3
更新日期:2002-06-01 00:00:00
abstract::Autoantibodies in the form of immune complexes are known to be crucial mediators in initiating inflammation in a variety of autoimmune diseases. This has been well documented in the anti-collagen II antibody-induced arthritis animal model for a long time now. Recently, in the K/B x N mouse model (the F1 of the TCR-tra...
journal_title:European journal of immunology
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doi:10.1002/eji.200526167
更新日期:2005-10-01 00:00:00
abstract::We have shown previously that complement factor H (CFH) and complement factor B (CFB) are constitutively expressed by retinal pigment epithelial cells and their production is regulated by inflammatory cytokines, suggesting that the alternative pathway (AP) of complement activation might play a role in retinal inflamma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040323
更新日期:2010-10-01 00:00:00
abstract::The tight-skin (Tsk/+) mutant mouse, a putative murine model of scleroderma, is characterized primarily by the excessive deposition of collagen and other extracellular matrix molecules in the dermis, and also by a developmentally acquired defect in pulmonary architecture. Passive transfer experiments have suggested an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2619::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::During the chronic phase of infection with the parasite Trypanosoma cruzi, mice develop inflammatory lesions in the heart and skeletal muscles, as well as in peripheral nerves and the liver. We demonstrated the presence, in the blood of chronically infected mice, of L3T4+ T cells able to transfer a specific T. cruzi d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170720
更新日期:1987-07-01 00:00:00
abstract::The cloned human gamma 1 heavy chain gene (HIG1) was scarcely expressed in the stable transformants of a mouse fibroblast line, L cell or a T cell line, EL4, and no gamma 1 heavy chain was produced in these cells. Upon treatment with cycloheximide or other kinds of protein synthesis inhibitors, the transcription of HI...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170906
更新日期:1987-09-01 00:00:00
abstract::Double-negative (DN) thymocyte subsets were examined in mice deficient in the CD3 zeta chain (zeta-/-). The HSA+CD44-CD25- subset was found to be missing, and DN thymocytes seemed to differentiate directly from HSA+CD25+CD44- cells to double-positive (DP) cells. When fetal thymic ontogeny was examined, we found a mark...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240828
更新日期:1994-08-01 00:00:00
abstract::Human cytomegalovirus (CMV) infection can be life threatening in the immune compromised and is associated with congenital defects and / or mental retardation in the neonate. The demonstrated association between CMV infection and rheumatoid factor (RF) raised the possibility of an induction of an autoimmune response up...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199911)29:11<3643::AID-IMM
更新日期:1999-11-01 00:00:00
abstract::Pre-B cells which show a delayed development of responsiveness to the B cell mitogen lipopolysaccharide (LPS) are found in murine embryonic blood between day 10 and 14 of gestation. The highest frequency of pre-B cells in embryonic blood is found at day 12 of gestation. All pre-B cells of embryonic blood, isolated at ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101007
更新日期:1980-10-01 00:00:00
abstract::We previously showed that granulocyte-macrophage colony-stimulating factor (GM-CSF) breaks tolerance induction. The objective of this study was to determine whether GM-CSF breaks established inhalation tolerance. To induce tolerance, BALB/c mice were exposed to aerosolized ovalbumin (OVA) for 10 consecutive days. A co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425012
更新日期:2004-09-01 00:00:00
abstract::Beta1 integrins can provide T cell co-stimulation, but little is known concerning their downstream signaling pathways. We found that Pyk2, a focal adhesion kinase-related tyrosine kinase, is regulated by beta1 integrin signaling in human T cells. Stimulation of Jurkat T cells with the alpha4beta1 integrin ligand VCAM-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3867::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::A number of applications of antibodies in diagnosis and therapy require multivalent reagents either because of the polymeric nature of the antigens or because biological activity depends on an effect on the formation of homodimeric species. Here, we report a procedure for mass screening of phage-derived monomeric anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270307
更新日期:1997-03-01 00:00:00
abstract::The influence of muramyl dipeptide (N-acetylmuramyl-L-alanyl-D-isoglutamine) on the rejection of mouse skin allografts was investigated. While the 0.1-mg dose administered on days 7, 6, 5 prior to transplantation caused significant prolongation of the graft survival, the 0.5-mg dose administered on days 3, 2, 1 prior ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131015
更新日期:1983-10-01 00:00:00
abstract::Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunizati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050612
更新日期:1976-06-01 00:00:00
abstract::Newborn mouse spleen, whose cells strongly suppress the in vitro humoral response of adult spleen cells, is essentially a hematopoietic organ. It contains a large percentage of proliferating cells, among which about 50% are erythroblasts (identified by their spectrin content) and about 15% are cells of the myelocytic ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110112
更新日期:1981-01-01 00:00:00
abstract::Polyclonal differentiation of unprimed B cells into IgM-producing cells induced by lipopolysaccharide (LPS) or T cell-derived lymphokine B151-TRF2 has been shown to contain a process of I-A-restricted B-B cell interaction, so that the B cell responses are inhibited by monoclonal antibodies (mAb) specific for I-A molec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191208
更新日期:1989-12-01 00:00:00
abstract::The binding of antigen to the multicomponent T cell antigen receptor (TcR)-CD3 complex leads to the activation of several signal transduction pathways which result in T lymphocyte proliferation and lymphokine secretion by molecular mechanisms and catalytic molecules as yet poorly defined. One of the earliest events th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220142
更新日期:1992-01-01 00:00:00
abstract::Plaque-forming cell (PFC) assays were used to investigate in vitro the immunoregulatory mechanism operating in the self-limiting anti-glomerular basement membrane (GBM) autoantibody response of Brown Norway (BN) rats given HgCl2. The peak splenic PFC response occurred at day 9; thereafter the response fell sharply and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140516
更新日期:1984-05-01 00:00:00
abstract::A generalized Shwartzman reaction was found to occur in non-SPF-NMRI mice given a local injection of S. marcescens lipopolysaccharide (LPS) in the footpad, followed, with an interval of 24 h, by an i.v. injection. The reaction occurred several hours after the second injection. It was characterized by disseminated subc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171228
更新日期:1987-12-01 00:00:00
abstract::The variable region nucleotide sequences of seven (five IgM and two IgG) anti-histone monoclonal antibodies from a single MRL/Mp-lpr/lpr mouse have been determined. These antibodies are not clonally related and used diverse V, D and J genes. However, six of the seven antibodies have VH segments encoded by genes from t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210721
更新日期:1991-07-01 00:00:00
abstract::We generated cell hybrids between mouse T cell lymphoma EL4 cells and mouse fibroblast B82 cells (BELIII and BELIV) to examine the expression of T cell receptor (TcR)/CD3 genes and their lymphoid-specific transcription factor genes, which are normally detected in EL4 cells. In BELIII and BELIV, expression of the TcR a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250947
更新日期:1995-09-01 00:00:00
abstract::CBA/J mice immunized with thyroglobulin (Tg) develop an experimental autoimmune thyroiditis (EAT) with lymphocytic infiltration of the thyroid glands, autoantibodies to Tg and occurrence of EAT-specific T cells. When these mice were treated for 4 weeks after immunization with 1 mg/week of a monoclonal antibody (mAb) t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230143
更新日期:1993-01-01 00:00:00
abstract::This study reports on the earliest stages of mononuclear phagocyte differentiation. A crucial question in this developmental process is whether mature macrophage (M phi) heterogeneity is already appointed at the precursor cell level. For this purpose, we produced clonal populations of mononuclear phagocytes from bone ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200104
更新日期:1990-01-01 00:00:00
abstract::The magnitude of the output of lymphocytes from Peyer's patches (PP), the morphology of the lymphocytes and their route of exit from PP has been examined in sheep. An extracorporeal perfusion system was used to selectively label a 3-4 m length of the terminal ileum of lambs at 6-10 weeks of age (the mesenteric lymph n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140103
更新日期:1984-01-01 00:00:00
abstract::For long-term attack on tumor cells in patients with prostate cancer, induction of cytolytic T cells is desirable. Several lineage-specific target proteins are known and algorithms have identified candidate MHC class I-binding peptides, particularly for HLA-A*0201. We have designed tolerance-breaking DNA fusion vaccin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141518
更新日期:2011-08-01 00:00:00