Abstract:
:For long-term attack on tumor cells in patients with prostate cancer, induction of cytolytic T cells is desirable. Several lineage-specific target proteins are known and algorithms have identified candidate MHC class I-binding peptides, particularly for HLA-A*0201. We have designed tolerance-breaking DNA fusion vaccines incorporating a domain of tetanus toxin fused to candidate tumor-derived peptide sequences. Using three separate peptide sequences from prostate-specific membrane antigen (PSMA) (peptides PSMA(27) , PSMA(663) , and PSMA(711) ), this vaccine design induced high levels of CD8(+) T cells against each peptide in a HLA-A(*) 0201 preclinical model. In contrast, the full-length PSMA sequence containing all three epitopes was poorly immunogenic. Induced T cells were cytotoxic against peptide-loaded tumor cells, but only those against PSMA(27) or PSMA(663) peptides, and not PSMA(711) , were able to kill tumor cells expressing endogenous PSMA. Cytotoxicity was also evident in vivo. The preclinical model provides a powerful tool for generating CD8(+) T cells able to predict whether target cells can process and present peptides, essential for planning peptide vaccine-based clinical trials.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Vittes GE,Harden EL,Ottensmeier CH,Rice J,Stevenson FKdoi
10.1002/eji.201141518subject
Has Abstractpub_date
2011-08-01 00:00:00pages
2447-56issue
8eissn
0014-2980issn
1521-4141journal_volume
41pub_type
杂志文章abstract::The human T cell leukemia virus type-1 (HTLV-1) is the etiologic agent of adult T cell leukemia (ATL). Since the HTLV-I-encoded transactivator Tax has been shown to activate many cellular genes including cytokine genes interleukin (IL-)1alpha, 2, 5, 6, 8, 10 and 15, we ask whether Tax also affects IL-4 expression. In ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200109)31:9<2623::aid-immu2623>3
更新日期:2001-09-01 00:00:00
abstract::Strains CBA (M523) (= M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B 10.D2 (M504 (= M504) and b 10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CB...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090208
更新日期:1979-02-01 00:00:00
abstract::ICAM-1 is a cell surface glycoprotein which is one of the ligands for the leukocyte function-associated antigen (LFA-1). It is involved in leukocyte adhesion to endothelial cells as well as in immune functions requiring cell-cell contact. The quantitative expression of ICAM-1 in various cell types can be either induce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201210
更新日期:1990-12-01 00:00:00
abstract::Lymphocyte infiltration to pancreatic islets is associated to chemoattraction, as are other inflammatory autoimmune processes. We examined whether development of insulitis and diabetes depends on chemoattraction of lymphocytes via the CCR5 chemokine receptor. In non-obese diabetic (NOD) mice, a substantial fraction of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324285
更新日期:2004-02-01 00:00:00
abstract::The recent discovery of IL-17-producing CD4(+) Th subset significantly revised the Th1/Th2 dichotomy model proposed by Mosmann and Coffman almost two decades ago. Th17 cells are involved in the pathogenesis of many human autoimmune diseases. Th17 cells, their developmental pathways and their effector functions, theref...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839105
更新日期:2009-03-01 00:00:00
abstract::In the thymus, in order to become MHC-restricted self-tolerant T cells, developing thymocytes need to interact with cortical and medullary thymic epithelial cells (TECs). Although the presence of a common bipotent progenitor for these functionally and structurally distinct epithelial subsets has been clearly establish...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201343323
更新日期:2013-03-01 00:00:00
abstract::Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the mole...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838689
更新日期:2009-03-01 00:00:00
abstract::While the nasal mucosa is a potentially useful site for human immunization, toxin-based nasal adjuvants are generally unsafe and less effective in humans. Safe mucosal adjuvants that activate protective immunity via mucosal administration are highly dependent on barrier antigen sampling by epithelial and DCs. Here, we...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142346
更新日期:2012-08-01 00:00:00
abstract::The cytotoxic response of cell sorter-purified small Lyt-2+ splenic cytotoxic lymphocyte precursors from 10 individual C57BL/6 mice to mutant class I H-2Kbm1 or H-2Dbm14 allodeterminants was analyzed under limiting dilution conditions. The cytotoxic activity of anti-bm1-specific or anti-bm14-specific cytotoxic T lymph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161220
更新日期:1986-12-01 00:00:00
abstract::The contribution of T cell responses to immunity against respiratory syncytial virus (RSV) is not fully defined, but this is an important issue for vaccine design. Recent studies demonstrating RSV-induced pulmonary T cell suppression suggest that RSV may have evolved strategies to escape T cell immunity. Here we evalu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200209)32:9<2562::AID-IMMU2562>3
更新日期:2002-09-01 00:00:00
abstract::Large granular lymphocyte clones were prepared from normal human mononuclear cells. Seven clones were studied in detail. All had high cytotoxic activity against the natural killer (NK) cell target K562 and all were T11- and DR-positive but T4- and T8-negative: none released migration inhibitory factor, a characteristi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150614
更新日期:1985-06-01 00:00:00
abstract::Accumulating evidence suggests that Th17 cells and Tregs may exhibit development plasticity and that CD4(+) Tregs can differentiate into IL-17-producing T cells; however, whether Th17 cells can reciprocally convert into Tregs has not been described. In this study, we generated Th17 clones from tumor-infiltrating T lym...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040682
更新日期:2011-04-01 00:00:00
abstract::To clarify reasons of the constant presence of IgM in human cord blood, some functions of this immunoglobulin class were analyzed. Cord IgM was collected from cord blood, and the cord IgM F(ab')2 fragment was obtained by trypsin cleavage. Maternal T and adult T cells were stained with fluorescein isothiocyanate (FITC)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110208
更新日期:1981-02-01 00:00:00
abstract::LIGHT is a recently identified member of the TNF superfamily that is up-regulated on activated T cells and can cooperate with CD40L to condition DC for the priming of CTL. In this report, we show that an intracellular pool of LIGHT is found selectively in some CD45RA-CD27+CD4+ "central" memory T cells and to a greater...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324410
更新日期:2003-11-01 00:00:00
abstract::We have developed a long-term culture system using the murine bone marrow stromal cells MS-5 to support the growth of progenitor B cells with CD34-, CD10+, CD19+, and cytoplasmic mu chain (C mu)-negative surface phenotype from human CD34+ cells purified from umbilical cord blood (CB). When 10(3) CD34+ cells/well were ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<855::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::It is widely believed that IL-4 exerts its influence by profiling the immune response during priming and expansion of immune cells, and thereby modulates the outcome of chronic inflammation. In the present investigation, collagen antibody-induced arthritis (CAIA) was used to delineate the role of IL-4 in a T cell-inde...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535633
更新日期:2006-06-01 00:00:00
abstract::Somatic hypermutation in B cells undergoing T cell dependent immune responses generates high affinity antibodies that provide protective immunity. B cells also switch from the expression of immunoglobulin (Ig) M and IgD to that of other Ig classes through somatic DNA recombination. Recent work has implicated DNA stran...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200202)32:2<316::AID-IMMU316>3.0
更新日期:2002-02-01 00:00:00
abstract::Membrane-coated microvesicles (MVs) have been identified as important mediators in intercellular communication. During the process of apoptosis, dying cells dynamically release MVs. Neutrophils are the most abundant type of leukocytes in the circulation. Due to their very short lifespan, it is likely that they are the...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546203
更新日期:2017-05-01 00:00:00
abstract::The human butyrophilin (BTN) 3 or CD277 molecules belong to the B7 family members and are expressed in various immune cells such as T and NK cells. Here, we show that CD277 triggering considerably enhances TCR-induced cytokine production and cell proliferation, even when another co-stimulatory molecule, CD28, is engag...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141404
更新日期:2011-12-01 00:00:00
abstract::IL-10 plays an important role in the regulation of immune responses. We and others have demonstrated recently that cyclic adenosine monophosphate (cAMP)-elevating substances up-regulate monocytic IL-10 expression in vitro and in vivo. Computer analysis of the IL-10 promoter/enhancer region localized four putative cAMP...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3098::AID-IMM
更新日期:1999-10-01 00:00:00
abstract::Mucosa-associated lymphoid tissue 1 (Malt1) regulates immune cell function by mediating the activation of nuclear factor κB (NF-κB) signaling through both its adaptor and proteolytic function. Malt1 is also a target of its own protease activity and this self-cleavage further contributes to NF-κB activity. Until now, t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847597
更新日期:2018-10-01 00:00:00
abstract::The delivery of T-cell help to B cells is antigen-specific, MHC-restricted, and CD40L (CD154) dependent. It has been thought that when a T cell recognizes an antigen-presenting B cell, CD40L expressed on the T-cell surface engages with CD40 on the surface of B cells as long as the cells remain conjugated. By adding fl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646504
更新日期:2017-01-01 00:00:00
abstract::F1----parental bone marrow chimeras between CBA and B10 mice were used to show that even after 1 year follicular dendritic cells (FDC), also termed dendritic reticular cells, are not derived from bone marrow stem cells. However clusters of cells wrapped in the dendritic processes of FDC, isolated by enzyme digestion o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140916
更新日期:1984-09-01 00:00:00
abstract::P-glycoprotein (P-gly) is the transmembrane efflux pump responsible for multidrug resistance in tumor cells. The activity of P-gly in mature peripheral lymphocytes is lineage specific, with CD8+ T cells and natural killer (NK) cells expressing high levels as compared to CD4+ T cells and B cells. We have now investigat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250549
更新日期:1995-05-01 00:00:00
abstract::B cell development in organ cultures of fetal liver from mice at day 14 of gestation resembles in kinetics and cell numbers generated the one observed in vivo. This development in vitro can be blocked by an IL-7 receptor-specific monoclonal antibody. Monoclonal antibodies specific for the pre-B cell receptor, i. e. fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200003)30:3<759::AID-IMMU759>3.0
更新日期:2000-03-01 00:00:00
abstract::Interferon (IFN) was detected upon co-culture of cloned Sendai virus (SV)-specific T lymphocyte with SV-presenting syngeneic stimulator cells. The antiviral activity was defined as IFN-alpha, beta. The T cell clones, upon contact with antigen-presenting stimulator cells, stimulated adherent cells present in the stimul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121212
更新日期:1982-12-01 00:00:00
abstract::Development of Th1 and Th2 effector lymphocytes is driven primarily by IL-12 or IL-4, but is also influenced by the strength of antigenic stimulation. However, the mechanism by which TCR signaling contributes to T cell polarization remains elusive. We show that in the presence of IL-12 a short TCR stimulation can lead...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4092::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::In chickens, the pre-B cell development takes place in a specialized organ, the bursa of Fabricius. In this study, we show that fibronectin can be detected in the bursa at least from the day 15 of embryonic incubation up to 10 weeks of age after which the bursa starts to involute. During the embryonic incubation, fibr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230323
更新日期:1993-03-01 00:00:00
abstract::Tissue factor (TF) is a transmembrane glycoprotein that plays an essential role in hemostasis by activating coagulation. TF is also expressed by monocytes/macrophages as part of the innate immune response to infections. In the current study, we determined the role of TF expressed by myeloid cells during Mycobacterium ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545817
更新日期:2016-02-01 00:00:00
abstract::Durch Pflanzenkrankheiten entstehen weltweit hohe Ernteverluste. Eine der häufigsten Infektionen in der Familie der Kreuzb&luml;tengewächse wird durch den Pilz Plasmodiophora brassicae verursacht. Die Krankheit äußert sich in Form von Wurzeltumoren, den "Kohlherniegallen". Da es auf verseuchten Böden zu hohen Ernteaus...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(sici)1521-415x(200001)30:1<14::aid-biuz14
更新日期:2000-01-01 00:00:00