Abstract:
:The variable region nucleotide sequences of seven (five IgM and two IgG) anti-histone monoclonal antibodies from a single MRL/Mp-lpr/lpr mouse have been determined. These antibodies are not clonally related and used diverse V, D and J genes. However, six of the seven antibodies have VH segments encoded by genes from the J558 family, two of these (an IgM and an IgG) share an identical VH gene. The isoelectric points of MRA3 and MRA12, the two IgG antibodies of the panel, range from 6.3 to 7.0 and from 6.0 to 6.3, respectively. The second conplementarity-determining region (CDR) of the VH gene of MRA12 (the most acidic and the most strongly histone-reactive antibody) includes only two positively charged but five negatively charged amino acid residues. This feature is unusual since the equivalent CDR in most VHJ558 genes are not comprised predominantly of acidic residues and suggests that such negatively charged residues are important for antibody binding to histones.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Monestier Mdoi
10.1002/eji.1830210721subject
Has Abstractpub_date
1991-07-01 00:00:00pages
1725-31issue
7eissn
0014-2980issn
1521-4141journal_volume
21pub_type
杂志文章abstract::The crucial role of interleukin (IL)-4 in the induction of murine IgG1 and IgE responses, which are coupled through the process of sequential isotype switching, has been well documented. Whereas IL-4 is obligatory for the induction of IgE responses, it enhances IgG1 responses. In this study, using neutralizing antibod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240624
更新日期:1994-06-01 00:00:00
abstract::One of the clear paradoxes in tumor immunology is the fact that cross-presentation of cell-associated tumor antigens to CD8(+) T cells is efficient, yet CTL generation is weak, and tumors continue to grow. We examined, for the first time whether this may be due to alterations in the phenotype or function of cross-pres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940153
更新日期:2010-06-01 00:00:00
abstract::To determine whether antigen (Ag)-specific cytotoxic T lymphocytes are generated during experimental Candida albicans infection, purified L3T4+ and Ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, C. albicans Ag, and interleukin 2. Yeast-infected bone marrow macrophages...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210636
更新日期:1991-06-01 00:00:00
abstract::Delivery of foreign antigens to mucosal surfaces, such as the pulmonary airways, has been shown to preferentially induce Th2-mediated responses in humans and in mice. What is not clear from these studies is whether this preferential skewing in responses is the result of the limited types of antigen being administered ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200003)30:3<840::AID-IMMU840>3.0
更新日期:2000-03-01 00:00:00
abstract::The mer receptor tyrosine kinase mediates phagocytosis of apoptotic cells and modulates cytokine production; it is also required for prevention of systemic autoimmune disease. Using a mer-specific antibody, we have confirmed the presence of mer on macrophages and now report its expression on NK cells, NKT cells, and d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324076
更新日期:2003-08-01 00:00:00
abstract::The in vitro recognition of acetylsalicilyl ovalbumin, penicilloylated bovine IgG (BPO-BGG) and the multichain copolymer poly-L-(Tyr, Glu)-poly-D L-Alapoly-L-Lys [(T, G)-A-L] by primed (2 x 13) F1 cells could be markedly and specifically blocked by alloantisera directed against "receptors" (R) or recognition structure...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830051012
更新日期:1975-10-01 00:00:00
abstract::Psoriasis is an inflammatory disease of the skin affecting 2-3% of the population, characterized by a thickening of the epidermis and immune infiltrates throughout the dermis and epidermis, causing skin lesions that can seriously affect quality of life. The study of psoriasis has historically been hampered by the lack...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343801
更新日期:2013-12-01 00:00:00
abstract::Chemokines are chemotactic cytokines with a key role in the control of cell trafficking and positioning under homeostatic and inflammatory conditions. D6 is a promiscuous 7-transmembrane-domain receptor expressed on lymphatic vessels which recognizes most inflammatory, but not homeostatic, CC chemokines. In vitro expe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526114
更新日期:2005-05-01 00:00:00
abstract::By combining velocity and linear density fractionations as well as target cell rosetting techniques we have isolated and morphologically identified the human effector cell type responsible for spontaneous, trypsin-augmentable cytotoxicity against chicken red cells and human myeloma cell line targets. This cell is a la...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830071104
更新日期:1977-11-01 00:00:00
abstract::The role of redox regulation in immune-mediated arthritis has been previously described. However, the relationship between innate immune cells, including innate lymphoid cells (ILCs) and phagocyte-derived ROS, in this process remains unclear. Here, we characterize ILCs and measure the IL-1 family cytokines along with ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948141
更新日期:2019-11-01 00:00:00
abstract::Reactive nitrogen intermediates (RNI), like nitric oxide (NO) and peroxynitrite, have antiviral effects against certain viruses. Hantaviruses, like other members of the Bunyaviridae family, have previously not been shown to be sensitive to RNI. In this study, we compared the effects of NO and peroxynitrite on hantavir...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535587
更新日期:2006-10-01 00:00:00
abstract::Mismatch repair contributes to hypermutation in B lymphocytes, both by increasing the frequency of mutations and by changing the mutational patterns. In this paper, we investigated whether or not mismatch repair influences activation-induced cytidine deaminase (AID)-mediated hypermutation in a non-B lymphocyte line. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526221
更新日期:2005-07-01 00:00:00
abstract::T-cell destiny during thymic selection depends on the affinity of the TCR for autologous peptide ligands presented in the context of MHC molecules. This is a delicately balanced process; robust binding leads to negative selection, yet some affinity for the antigen complex is required for positive selection. All TCRs o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242606
更新日期:2012-12-01 00:00:00
abstract::Anti-peroxidase antibody (Ab)-secreting hybrids have been produced by fusion of peroxidase (PO)-immunized mouse lymph node cells and immunoglobulin (Ig)-secreting P3-X63-Ag8 (X63) myeloma cells. Identification of Ab-secreting hybrids can be performed as early as day 5 after cell fusion by the hemolytic plaque assay. I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090102
更新日期:1979-01-01 00:00:00
abstract::For homeostasis, T cells integrate non-cognate TCR-dependent and -independent signals to survive and weakly proliferate. In contrast to antigen-specific, stable, and long-lived contacts, signaling in short-lived homeostatic interactions depends upon the coordination of ongoing T-cell migration on the surface of DC and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040575
更新日期:2010-10-01 00:00:00
abstract::Murine splenic T lymphocytes display maximal cellular myc gene (c-myc) expression already 3 h after concanavalin A stimulation and subsequent down-regulation before the onset of DNA synthesis. Stimulation by leucoagglutinin in the presence or absence of interleukin 2 leads to only low initial levels of c-myc-specific ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160319
更新日期:1986-03-01 00:00:00
abstract::IL-15 is involved in lymphocyte homeostasis. To investigate the role of IL-15 in the skin in vivo, mice were generated that overexpress IL-15 in keratinocytes, resulting in increased IL-15 protein levels in the skin but not elevated IL-15 serum concentrations. Keratin 14 (K14)-IL-15 transgenic (tg) mice showed increas...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324785
更新日期:2004-07-01 00:00:00
abstract::Normal BALB/c spleen cells incubated for 2 days with Streptococcus pneumoniae R 36 a (Pn) vaccine in vitro acquired the specific ability to inhibit the T-independent anti-Pn response of fresh lymphocytes. The activated suppressor cells were susceptible to cytotoxic treatment with mouse anti-theta serum plus complement...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100902
更新日期:1980-09-01 00:00:00
abstract::Studies of experimental autoimmune encephalomyelitis (EAE) in rodents have revealed that encephalitogenic T cell lines reactive with myelin basic protein (BP) are frequently dominated by clones expressing a restricted T cell receptor repertoire. Using the rat EAE model, we have begun to examine the basis for clonal do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250114
更新日期:1995-01-01 00:00:00
abstract::PKCε is implicated in T cell activation and proliferation and is overexpressed in CD4+ -T cells from patients with autoimmune Hashimoto's thyroiditis. Although this might induce the suspicion that PKCε takes part in autoimmunity, its role in the molecular pathophysiology of immune-mediated disorders is still largely u...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747102
更新日期:2018-04-01 00:00:00
abstract::Caspases are essential mediators of cytokine release and apoptosis. Additionally, caspase activity is required for the proliferation of naive T lymphocytes. It remained unclear how proliferating cells are able to cope with the pro-apoptotic activity especially of effector caspases-3 and -7. Possible reasons might incl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838211
更新日期:2008-07-01 00:00:00
abstract::The human type I interferon (IFN) receptor was characterized by ligand blotting. In this method, plasmalemma proteins or detergent-lysed whole-cell extracts from human Burkitt lymphoma cell lines were separated on polyacrylamide gels and subsequently transferred onto nitrocellulose sheets. Probing the blots with 3 x 1...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181221
更新日期:1988-12-01 00:00:00
abstract::We have previously demonstrated that mycobacterial lipoproteins engage TLR2 on human CD4(+) T cells and upregulate TCR-triggered IFN-γ secretion and cell proliferation in vitro. Here we examined the role of CD4(+) T-cell-expressed TLR2 in Mycobacterium tuberculosis (MTB) Ag-specific T-cell priming and in protection ag...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344100
更新日期:2014-05-01 00:00:00
abstract::Human peripheral blood T lymphocytes are stimulated to grow and divide by lectins such as concanavalin A (Con A) and Phaseolus vulgaris phytohemagglutinin (PHA), as well as a few anti-T cell monoclonal antibodies. The latter antibodies recognize the T3 antigen. It has been suggested previously that PHA and Con A media...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150512
更新日期:1985-05-01 00:00:00
abstract::Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency is common in young adul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343940
更新日期:2014-03-01 00:00:00
abstract::A fraction of activated CD8+ T cells expresses CD40 ligand (CD40L), a molecule that plays a key role in T cell-dependent B cell stimulation. CD8+ T cell clones were examined for CD40L expression and for their capacity to allow the growth and differentiation of B cells, upon activation with immobilized anti-CD3. Accord...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251039
更新日期:1995-10-01 00:00:00
abstract::The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night cu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260630
更新日期:1996-06-01 00:00:00
abstract::Treg subsets play a role in sustaining peripheral tolerance, are characterized by markers such as forkhead winged-helix transcription factor (FOXP3) and CD25, and produce suppressive cytokines, such as IL-10 and TGF-β. Glucocorticoid-induced TNF receptor family-related (GITR) protein has been suggested to regulate Tre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040943
更新日期:2011-08-01 00:00:00
abstract::MS is a multifactorial disease in which a series of genetic and non-genetic, environmental factors plays a role in its etiology. In particular, HLA class II alleles, mainly HLADRB1*15:01 (HLA-DR15), increase the risk for this disease. Out of several environmental factors, and with regard to infections, EBV remains to ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202049030
更新日期:2020-12-22 00:00:00
abstract::IL-12 is a potent inducer of IFN-gamma production and drives the development of Th1 cells. Human polarized Th2 cells do not express the signaling beta2-subunit of the IL-12R and, therefore, do not signal in response to IL-12. The question was raised as to what extent the loss of the IL-12Rbeta2 chain in Th2 cells has ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200104)31:4<1055::aid-immu1055>3
更新日期:2001-04-01 00:00:00