Abstract:
:Mismatch repair contributes to hypermutation in B lymphocytes, both by increasing the frequency of mutations and by changing the mutational patterns. In this paper, we investigated whether or not mismatch repair influences activation-induced cytidine deaminase (AID)-mediated hypermutation in a non-B lymphocyte line. We did so by regulating expression of MutL homologue MLH 1, which is essential in mismatch repair, in a kidney cell line that had been transduced by an AID-containing vector. Whether or not MLH1 was expressed, we found no difference in the mutation rates of an indicator gene. We conclude that in order to contribute to hypermutation, mismatch repair needs additional factors that are present in activated B lymphocytes, but absent in the cell line investigated.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Klasen M,Spillmann FJ,Marra G,Cejka P,Wabl Mdoi
10.1002/eji.200526221keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
2222-9issue
7eissn
0014-2980issn
1521-4141journal_volume
35pub_type
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