Abstract:
:We generated cell hybrids between mouse T cell lymphoma EL4 cells and mouse fibroblast B82 cells (BELIII and BELIV) to examine the expression of T cell receptor (TcR)/CD3 genes and their lymphoid-specific transcription factor genes, which are normally detected in EL4 cells. In BELIII and BELIV, expression of the TcR alpha, TcR beta and CD3 delta genes was extinguished, whereas expression of the CD3 epsilon gene was still detected. Expression of the (lymphoid enhancer binding factor 1) LEF-1 gene was extinguished and that of the GATA-3 gene was hardly detected in BELIII and BELIV. Ets-1 gene expression, observed not only in EL4 cells but also in B82 cells, was considerably reduced in BELIII and BELIV. A much higher level of PEBP2 alpha A gene expression was observed in B82 cells than in EL4 cells and was preserved in BELIII and BELIV. To examine whether reduced expression of these genes is also found in T cell x B cell hybrids, we generated an additional cell hybrid between EL4 cells and mouse plasmacytoma S194 cells (SELIII). Marked differences were observed in the expression of the TcR alpha, CD3 delta, LEF-1 and PEBP2 alpha A genes in BEL and SEL hybrids. Expression of the TcR alpha, CD3 delta and LEF-1 genes, which was extinguished in BELIII and BELIV, was detected in SELIII. PEBP2 alpha A gene expression, not detected in S194 cells, was considerably reduced in SELIII. Almost the sum of the chromosomes from the parental cells were retained by, and the presence of every gene was proven, in each cell hybrid. These results suggest that suppression of the expression of lymphoid-specific transcription factor genes may precede that of the TcR/CD3 genes in the cell hybrids, and that the presence of a different trans-acting negative regulatory mechanism(s) suppresses the expression of T cell specific genes in fibroblasts and B cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Yamada T,Hitomi Y,Satake M,Oikawa Tdoi
10.1002/eji.1830250947subject
Has Abstractpub_date
1995-09-01 00:00:00pages
2710-3issue
9eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::The activity of granzyme B, a main effector molecule of cytotoxic T lymphocytes (CTL) and natural killer cells, is regulated by the human intracellular serpin proteinase inhibitor 9 (PI9). This inhibitor is particularly expressed by CTL and dendritic cells, in which it serves to protect these cells against endogenous ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425949
更新日期:2005-04-01 00:00:00
abstract::The cell surface expression and biosynthesis of Langerhans cells (LC)-derived major histocompatibility complex (MHC) class II molecules from epidermal cells (EC) prepared freshly and cultured for up to 3 days was investigated. Based on the constitutive expression of MHC class II determinants by LC, a panning and magne...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210518
更新日期:1991-05-01 00:00:00
abstract::The regulation of nuclear factor (NF)-kappa B activation by the T cell receptor (TcR)/CD3 complex in primary human T cells has been studied at various times after activation. Only p50 NF-kappa B protein bound the kappa B element of interleukin-2 receptor (IL-2R) alpha chain promoter on resting T cells. However, immedi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250130
更新日期:1995-01-01 00:00:00
abstract::Glomerular crescent formation characterizes severe glomerulonephritis (GN). Evidence suggests that crescent formation results from a delayed-type hypersensitivity-like Th1 response. As IL-12 directs Th1 responses, we tested the hypothesis that IL-12 is important in crescentic GN. Neutralization of IL-12 attenuated cre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199901)29:01<1::AID-IMMU1>
更新日期:1999-01-01 00:00:00
abstract::Thymic nurse cells, cortical epithelial cells enclosing 20-200 lymphocytes, were prepared from mouse thymus by enzyme digestion and repetitive sedimentation. Individual nurse cells were then isolated free of any exogenous thymocytes by micromanipulation, and the endogeneous thymocytes released from inside the nurse ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151016
更新日期:1985-10-01 00:00:00
abstract::Mice lacking the tumor suppressor gene p53 spontaneously develop T-cell lymphomas at a high rate, suggesting that in these mice lymphomas arise due to defective apoptosis mechanisms in T cells mediated by p53. However, a role of p53 in regulation of T-cell responses or apoptosis has been poorly defined. TCR-mediated s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939736
更新日期:2010-02-01 00:00:00
abstract::Normal immunosuppressive protein, prepared from human plasma by DEAE-cellulose chromatography, inhibits DNA synthesis in human cell lines of lymphocytes of both T and B origin. It also inhibits [3H]thymidine incorporation in mouse cell lines. Normal immunosuppressive protein was able to inhibit the proliferation of th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090216
更新日期:1979-02-01 00:00:00
abstract::The mast cell function-associated antigen (MAFA) is a membrane glycoprotein first identified on rat mucosal type mast cells (line RBL-2H3) and known to inhibit the Fc epsilon RI-mediated secretory response. In its extracellular domain, an amino acid stretch homologous to the carbohydrate binding domain of calcium-depe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271014
更新日期:1997-10-01 00:00:00
abstract::T-cell development is a spatially and temporally regulated process, orchestrated by well-defined contributions of transcription factors and cytokines. Here, we identify the noncoding RNA miR-142 as an additional regulatory layer within murine thymocyte development and proliferation. MiR-142 deficiency impairs the expr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201746987
更新日期:2017-07-01 00:00:00
abstract::Immunotherapy generally fails to induce tumour regression in spontaneously arising tumours. Failure is attributed to both tumour-related factors and an ineffective immune response. As a model of tumour immunotherapy, without the confounding effects of potential tumour-determined mechanisms of immune evasion, we studie...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737599
更新日期:2008-01-01 00:00:00
abstract::Thymectomy was performed on the urodele amphibian Pleurodeles waltlii Michah. from the 5th to the 20th week of larval development. Thymectomized animals were allografted at 7 months of age. The results obtained are as follows: (1) Pleurodeles thymectomized before 10 weeks always tolerated allografts: (2) Pleurodeles t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050215
更新日期:1975-02-01 00:00:00
abstract::CD16 is an IgG Fc receptor that is predominantly expressed on human natural killer (NK) cells and granulocytes. The CD16 antigen expressed on neutrophils is a 50 to 70-kDa glycoprotein attached to the plasma membrane by a phosphatidylinositol-glycan linkage that is susceptible to cleavage by phosphatidylinositol-speci...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190431
更新日期:1989-04-01 00:00:00
abstract::Natural antibodies (Ab) reacting with self antigens have been shown to be present in all individuals. These autoantibodies (auto-Ab) can be either pathogenic or non-pathogenic. Auto-Ab reacting with the alpha-subunit of the high-affinity receptor for IgE (FcepsilonRIalpha) have been implicated in the pathogenesis of a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1149::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::The immune system relies on homeostatic mechanisms in order to adapt to the changing requirements encountered during steady-state existence and activation by antigen. For T cells, this involves maintenance of a diverse repertoire of naïve cells, rapid elimination of effector cells after pathogen clearance, and long-te...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200939444
更新日期:2009-08-01 00:00:00
abstract::Adoptive lymphocyte transfers between Iga, Igb and Igd allotype-congenic mouse strains revealed host barriers against the production of certain donor allotypes. First, as recipients of Igb cells, Iga and Igd mice permitted the production of donor Ig-4b but not that of Ig-1 b. The apparent mediators of this Ig-1 b barr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080805
更新日期:1978-08-01 00:00:00
abstract::Central and peripheral tolerance mechanisms are expected to hamper the generation of effective immunity against tumors. To break self tolerance against malignant gliomas, we assessed the therapeutic potential of self/foreign antigen cross-reactivity in an immunocompetent rat glioma model. Immunotherapy of tumors using...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200390005
更新日期:2003-01-01 00:00:00
abstract::Impaired control of chronic pathogen replication may be associated to alterations of NK-cell function. Whether mechanisms underlying this dysfunction involve perturbations of differentiating NK cells is still unknown. We studied an "in vitro" model of differentiation from CD34(+)Lin(-) precursors growing only myelomon...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838532
更新日期:2009-01-01 00:00:00
abstract::An association between T-cell lymphopenia and autoimmunity has long been proposed, but it remains to be elucidated whether T-cell lymphopenia affects B-cell responses to autoantigens. Human neonatal thymectomy (Tx) results in a decrease in T-cell numbers and we used this model to study the development of autoreactivit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201746971
更新日期:2017-11-01 00:00:00
abstract::A small dose of the IgG1 fraction of anti-idiotypic antibody (aId1) raised in guinea pigs against a strain A/J antibody specific for streptococcal Group A carbohydrate sensitizes A/J mice against Group A streptococci. This is opposed to the previously established suppressive function of anti-idiotypic antibody of the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830051002
更新日期:1975-10-01 00:00:00
abstract::The antigen receptor of the T lymphocytes is one of the most complex eukaryotic membrane structures studied to date. The T cell receptor (TcR) consists of two disulfide-linked glycoprotein chains (alpha/beta or gamma/delta) and is noncovalently associated with a group of small and invariable CD 3 proteins. Four CD 3 c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180508
更新日期:1988-05-01 00:00:00
abstract::An intracellular staining procedure for the cytoskeletal marker, glial fibrillary acidic protein of astrocytes, has been developed which allows flow cytometric phenotyping of astrocytes within complex mixtures of glial cells. Employing this technique, we show here that measles virus infection of rat mixed glial cell c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220126
更新日期:1992-01-01 00:00:00
abstract::Accumulating evidence suggest that infection with Chlamydia pneumoniae is associated with atherosclerosis, but the mechanisms involved remain unclear. Inflammation is important in the initial phase of atherogenesis, and cytokines are important in the initiation and progression of inflammation. The aim of this study wa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<541::AID-IMMU541>3.0
更新日期:2000-02-01 00:00:00
abstract::Autophagy is an intracellular degradation system that plays an important role in T-cell survival. However, the precise mechanism linking autophagy and cell death in primary human T cells is unclear because methods for monitoring autophagy in small numbers of primary human cells remain controversial. We established a n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344248
更新日期:2014-08-01 00:00:00
abstract::In previous publications it was shown by avidity measurements, cross-reactivity patterns and genetic analyses, that the tetrapeptide T-T-G-G is the immuno-dominant epitope of the synthetic polypeptide (T, G)-A--L. In the present study this close immunological relationship between the random multichain copolymer (T, G)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060905
更新日期:1976-09-01 00:00:00
abstract::Tuberculosis (TB) is one of the world's most pernicious diseases mainly due to immune evasion strategies displayed by its causative agent Mycobacterium tuberculosis (Mtb). Blood monocytes (Mos) represent an important source of DCs during chronic infections; consequently, the alteration of their differentiation constit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242557
更新日期:2013-02-01 00:00:00
abstract::Mice carrying the H-2b haplotype (e.g., inbred strains C57BL/6 and C57BL/10) are low responders to sperm whale myoglobin when tested in the T cell proliferation assay. Their response is improved by the removal of the Ly-2+ cells from the lymph node population, but it still remains significantly lower than that of cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180905
更新日期:1988-09-01 00:00:00
abstract::Upon activation in vitro, only a fraction of the bulk human T helper cell cultures secret the hallmark Th1/2 cytokines (IFN-gamma for Th1 and IL-4 for Th2, respectively). It is uncertain whether these IFN-gamma-/IL-4- cells are differentiated Th1 or Th2 cells. Here, we have characterized live IFN-gamma+, IL-4+ and IFN...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425957
更新日期:2005-09-01 00:00:00
abstract::Propionibacterium acnes-primed mice develop an IFN-gamma-dependent hypersensitivity towards LPS. Since CD14 plays a key role in LPS-induced cell activation the regulation and function of CD14 in this sensitization process were studied in IFN-gamma R-/- and the respective wild-type (wt) mice. In unprimed mice, CD14 (mR...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200203)32:3<761::AID-IMMU761>3.0
更新日期:2002-03-01 00:00:00
abstract::The effector function of activated CD4(+) T cells and secretion of cytokines are important in the establishment of productive immune responses and tolerance. We identified expression by CD4(+) T cells of Notch receptors and ligands and enhanced Notch signaling upon activation. Notch1 expression was up regulated and co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425562
更新日期:2005-03-01 00:00:00
abstract::It is well accepted that Th17 cells are a highly plastic cell subset that can be easily directed toward the Th1 phenotype in vitro and also in vivo during inflammation. However, there is an ongoing debate regarding the reverse plasticity (conversion from Th1 to Th17). We show here that ectopic ROR-γt expression can re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847677
更新日期:2019-01-01 00:00:00