Abstract:
:Double-negative (DN) thymocyte subsets were examined in mice deficient in the CD3 zeta chain (zeta-/-). The HSA+CD44-CD25- subset was found to be missing, and DN thymocytes seemed to differentiate directly from HSA+CD25+CD44- cells to double-positive (DP) cells. When fetal thymic ontogeny was examined, we found a marked difference between zeta-/- embryos and heterozygous littermates from embryonic day 17.5, in terms of CD25, CD4 and CD8 expression, and thymus size. The zeta-/- thymocytes failed to down-regulate CD25 and to expand exponentially. The cell cycle status of adult thymocyte subsets indicated that although the HSA+CD25-CD44- subset was missing, the CD25+ DN population contained normal numbers of cycling cells, and the CD25+ DP cells (which were not detectable in normal mice) contained 5-10% cells in G2/M+S. Taken together these data suggest that the CD3 zeta chain might have a specific role in the control of proliferation of DN thymocytes during T cell development. Our data clearly show that one can dissociate the signal for a CD25+ DN cell to differentiate (which occurs in the absence of CD3 zeta), from a signal to proliferate and from loss of cell surface CD25.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Crompton T,Moore M,MacDonald HR,Malissen Bdoi
10.1002/eji.1830240828subject
Has Abstractpub_date
1994-08-01 00:00:00pages
1903-7issue
8eissn
0014-2980issn
1521-4141journal_volume
24pub_type
杂志文章abstract::The regulation of nuclear factor (NF)-kappa B activation by the T cell receptor (TcR)/CD3 complex in primary human T cells has been studied at various times after activation. Only p50 NF-kappa B protein bound the kappa B element of interleukin-2 receptor (IL-2R) alpha chain promoter on resting T cells. However, immedi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250130
更新日期:1995-01-01 00:00:00
abstract::Cervical thymus mimics the thoracic thymus in supporting T-cell development and exists in a subset of mice and humans. Importantly, it remains unknown whether the cervical thymus can generate T cells that are self-tolerant in the complete absence of signals from the thoracic thymus. Using a fetal liver reconstitution ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343846
更新日期:2014-08-01 00:00:00
abstract::There is limited insight into the mechanisms involved in the counterregulation of TLR. Given the important role of TLR3/TIR domain-containing adaptor-inducing IFN-β (TRIF)-dependent signalling in innate immunity, novel insights into its modulation is of significance in the context of many physiological and pathologica...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040547
更新日期:2010-11-01 00:00:00
abstract::Human herpesvirus 6B (HHV-6B) is a ubiquitous pathogen with frequent reactivation observed in immunocompromised patients such as BM transplant (BMT) recipients. Adoptive immunotherapy is a promising therapeutic avenue for the treatment of opportunistic infections, including herpesviruses. While T-cell immunotherapy ca...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/eji.201444931
更新日期:2014-12-01 00:00:00
abstract::In vitro prepared antigen-specific helper factors reactive to the synthetic polypeptide antigens poly-L(Tyr, Glu)-poly-DLAla--poly-LLys [(T, G)-A--L] or LGlu60-LAla30-LTyr10 (GAT) and bearing Ia determinants were analyzed serologically to determine the nature of the Ia determinants they expressed. I subregion-specific...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090703
更新日期:1979-07-01 00:00:00
abstract::To study the control of immunoglobulin kappa light chain gene rearrangement, we generated transgenic mice carrying a germ-line human kappa minilocus (HK) containing the J kappa-proximal V gene, V kappa IV, the V-J intergenic region, the five J kappa segments and the C kappa gene. This construct includes the intronic, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::A monoclonal antibody (12-15) reactive with the mouse CD2 was used to study the expression of the antigen in different lymphoid cell subsets. By two-color immunofluorescence using B or T cell-specific reagents and cell sorting in combination with biochemical analysis we provide evidence that the CD2 antigen is present...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190826
更新日期:1989-08-01 00:00:00
abstract::In hematopoietic stem cell transplantation (HSCT), when no HLA full-matched donor is available, alternative donors could include one HLA-mismatched donor. Recently, the low expressed HLA-C alleles have been identified as permissive mismatches for the best donor choice. Concerning HLA-A, the degree of variability of ex...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545927
更新日期:2015-12-01 00:00:00
abstract::The MRC OX-22 monoclonal antibody recognizes a restricted determinant of the rat leukocyte-common antigen (L-CA, CD45), which is expressed on most peripheral T cells and all B cells. In contrast only 2%-3% of thymocytes are OX-22+ and these are now shown to be mostly of the immature CD4-CD8- (double-negative, DN), phe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191217
更新日期:1989-12-01 00:00:00
abstract::Expression and function of the fractalkine receptor CX3CR1 by T lymphocyte subpopulations was evaluated in healthy individuals. In CD8(+) T lymphocytes, CX3CR1 was expressed by and functional in both CD45RO(-) and CD45RO(+) cells. In CD4(+) T lymphocytes, CX3CR1 was expressed mainly by CD45RO(+) cells, and almost excl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200001)30:1<87::AID-IMMU87>3.0.C
更新日期:2000-01-01 00:00:00
abstract::Monoclonal antibodies (mAb) derived from BALB/c mice immunized with human anti-HLA-A2 cloned cytotoxic T lymphocytes (CTL) were screened for their ability to block, in the absence of complement, the cytolytic activity of the immunizing CTL clone. Eight cytolysis-inhibiting mAb have been derived. One of these was direc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120908
更新日期:1982-09-01 00:00:00
abstract::The human T lymphocyte-specific CD2 (T11) molecule, that regulates T cell activation, is capable of mediating increases of intracellular cAMP. Monoclonal antibodies directed to different epitopes of the CD2 molecule which either induce or inhibit T cell proliferation are capable of triggering an increase of cAMP compa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180620
更新日期:1988-06-01 00:00:00
abstract::KARAP/DAP12 is a broadly distributed transmembrane signaling polypeptide with an immunoreceptor tyrosine-based activation motif, and is non-covalently associated with a variety of activating surface receptors. We report here the characterization of transgenic mice that overexpress KARAP/DAP12 polypeptides in both myel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200209)32:9<2653::AID-IMMU2653>3
更新日期:2002-09-01 00:00:00
abstract::Exosomes are nanovesicles produced constitutively and inducibly by several types of cells. They are generated as intraluminal vesicles of multivesicular bodies and express MHC and several endosomal/lysosomal proteins. In spite of their potential role in cellular immunity, the regulatory mechanisms of exosome productio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737694
更新日期:2008-05-01 00:00:00
abstract::Cytokine-induced memory-like (CIML) NK cells are endowed with the capacity to mediate enhanced effector functions upon cytokine or activating receptor restimulation for several weeks following short-term preactivation with IL-12, IL-15, and IL-18. Promising results from a first-in-human clinical trial highlighted the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847931
更新日期:2019-08-01 00:00:00
abstract::The modulatory activity of dextran sulfate with a relative molecular mass of 8 and 500 kDa and pentosan polysulfate with a relative molecular mass of 6 kDa on human T cell surface molecules CD2, CD3, CD4, CD8 and HLA-DR was investigated by analytical flow cytometry. The 500-kDa dextran sulfate induces a complete disap...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190630
更新日期:1989-06-01 00:00:00
abstract::In the present study, interleukin 1 (IL 1)-containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2 secretion by chondrocytes...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140603
更新日期:1984-06-01 00:00:00
abstract::The biological behavior of immune cells is determined by their intrinsic properties and interactions with other cell populations within their microenvironment. Several studies have confirmed the existence of tight spatial interactions between mast cells (MCs) and Tregs in different settings. For instance, we have rece...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041300
更新日期:2011-07-01 00:00:00
abstract::Interleukin (IL)-6 transsignaling plays a pivotal role in the shift from neutrophil to monocyte recruitment at the inflammatory site. Release of neutrophil IL-6 receptor-alpha (IL-6Ralpha, CD126) in its soluble form is a key step of IL-6 transsignaling, however, its physiological inducers are poorly known. Here, we ob...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2002010)32:10<2965::AID-IMMU2965
更新日期:2002-10-01 00:00:00
abstract::The thymus is the primary organ for T-cell differentiation and maturation. Unlike other major organs, the thymus is highly dynamic, capable of undergoing multiple rounds of almost complete atrophy followed by rapid restoration. The process of thymic atrophy, or involution, results in decreased thymopoiesis and emigrat...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201142305
更新日期:2012-05-01 00:00:00
abstract::Systemic lupus erythematosus is characterized by the presence of high titers of autoantibodies reacting with various components of the U1 small nuclear ribonucleoprotein particle (snRNP). It has been suggested that these antibodies are produced by an antigen-driven mechanism under the dependence of antigen-specific T ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2000)30:8<2191::AID-IMMU2191>3.0
更新日期:2000-08-01 00:00:00
abstract::Macrophage-chemotactic factor (MChF)-producing T cell hybridomas were established. These hybridomas produced MChF upon the specific antigenic stimulation with phosphorylcholine (PC)-conjugated protein in the presence of I-Ed-positive antigen-presenting cells. The hybridomas also secreted MChF after co-culture with con...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140804
更新日期:1984-08-01 00:00:00
abstract::The murine T cell response to heterologous insulins provides a good model system for studying the mechanism of immune response (Ir)-gene function, since insulin is a small, chemically well-defined molecule. H-2b mice respond predominantly to A chain loop determinants of beef insulin, presented by the I-A epitope Ia. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141208
更新日期:1984-12-01 00:00:00
abstract::Immunizations via the i.n. and intravaginal (ivag) routes effectively generate strong genital tract antibody-mediated immunity. To what extent the same is true for T-cell responses is incompletely known. Therefore, we set out to investigate optimal conditions for stimulation of genital tract CD4(+) T-cell responses, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041297
更新日期:2011-09-01 00:00:00
abstract::Activation-induced deaminase (AID) is the master regulator of class switch recombination (CSR) and somatic hypermutation (SHM), but the mechanisms regulating AID function are obscure. The differential pattern of switch plasmid activity in three IgM(+)/AID(+) and two IgG(+)/AID(+) B cell lines prompted an analysis of g...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737664
更新日期:2008-01-01 00:00:00
abstract::Vitamin A deficiency is known to be accompanied with immune deficiency and susceptibility to a wide range of infectious diseases. Experimental evidence suggests that the active metabolites of vitamin A that mediate its effects on the immune system are the retinoic acids (RA), which are ligands for the nuclear RA recep...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737342
更新日期:2008-01-01 00:00:00
abstract::Co-culture of purified T and B cells obtained from cytochrome c-specific TCR- and hen egg lysozyme (HEL)-specific Ig-transgenic mice was used to examine the role of B cell receptor (BCR) ligation and TCR affinity on the efficiency of T-B cell collaboration. The results showed that BCR ligation of naive B cells with HE...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<4037::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::Experimental allergic encephalomyelitis (EAE) is an animal model for the human disease, multiple sclerosis. The LEW rat strain is very susceptible to induction of EAE, whereas the closely related, major histocompatibility complex (MHC)-identical, inbred strain LER is resistant. In this report, the two rat strains have...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210910
更新日期:1991-09-01 00:00:00
abstract::Wiskott-Aldrich syndrome (WAS) is an X-linked disorder characterized by congenital thrombocytopenia and progressive deterioration of the immune function. Dendritic cells (DC) are key effectors in the induction of specific immunity and are highly specialized in antigen uptake and subsequent migration to draining lymph ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3413::aid-immu3413>
更新日期:2001-12-01 00:00:00
abstract::Precursor CD4-CD8- (DN) thymocytes rearrange their TCR-beta genes, and only those which succeed in beta-selection subsequently expand and differentiate into immature CD4+CD8+ (DP) thymocytes. The cell subsets corresponding to the successive steps of this transition can be defined in terms of CD44 and CD25 expression. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2001010)31:10<3038::aid-immu3038
更新日期:2001-10-01 00:00:00