Abstract:
:In the present study, interleukin 1 (IL 1)-containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2 secretion by chondrocytes. A high correlation was found between the enhancement of thymocyte proliferation and the induction of collagenase and PGE2 secretion by chondrocytes. Furthermore, a highly purified IL 1-like factor, namely mononuclear cell factor (MCF) was also active on chondrocytes. The addition of highly purified IL 2 to rabbit chondrocytes had no effect on collagenase and PGE2 secretion induced by IL 1-containing media. Our findings suggest that the factor which induced collagenase and PGE2 secretion by rabbit chondrocytes was an IL 1-like factor. Thus, collagenase secretion by chondrocytes may be used as an IL 2-insensitive assay for the detection of IL 1-like factors.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Evêquoz V,Bettens F,Kristensen F,Trechsel U,Stadler BM,Dayer JM,De Weck AL,Fleisch Hdoi
10.1002/eji.1830140603subject
Has Abstractpub_date
1984-06-01 00:00:00pages
490-5issue
6eissn
0014-2980issn
1521-4141journal_volume
14pub_type
杂志文章abstract::Splenic T cells were primed, after removal of alloreactive cells, to beef insulin on allogeneic antigen-presenting cells (APC). The fine specificity of in vitro secondary response was tested in combinations H-2b (responder) T cell-H-2k (nonresponder) APC, and vice versa, using separated chains of beef and pork insulin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130810
更新日期:1983-08-01 00:00:00
abstract::In a search for proteins involved in signal transduction through the T cell receptor (TcR/CD3 complex), a recently developed highly efficient method for labeling of nucleotide binding proteins in permeabilized cells was applied. Here, we report that human CD3-gamma could be labeled by periodate-oxidized [alpha-32P] GT...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230224
更新日期:1993-02-01 00:00:00
abstract::Development of Th1 and Th2 effector lymphocytes is driven primarily by IL-12 or IL-4, but is also influenced by the strength of antigenic stimulation. However, the mechanism by which TCR signaling contributes to T cell polarization remains elusive. We show that in the presence of IL-12 a short TCR stimulation can lead...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4092::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::MICA and MICB (MHC-class-I-related chain A/B) are transmembrane proteins expressed in pathological conditions that are ligands for NKG2D, an activating receptor found on cytotoxic lymphocytes. The recognition on target cells of NKG2D ligands leads to the activation of lysis and cytokine secretion by NK cells and T cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141645
更新日期:2011-12-01 00:00:00
abstract::Reciprocal expression of CD45RA and CD45RO in human CD4+ T cells defines populations understood to be naive cells (CD45RA+CD45RO-) and memory cells (CD45RA-CD45RO+). We investigate two subsets of CD45RA-CD45RO+ CD4+ human T cells which differ by fourfold in their expression of the CD45RB isoform; one is CD45RBbright a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240536
更新日期:1994-05-01 00:00:00
abstract::Dendritic cells (DC) are the main orchestrators of specific immune responses. Depending on microbial information they encounter in peripheral tissues, they promote the development of Th1, Th2 or unpolarized Th cell responses. In this study we have investigated the immunomodulatory effect of non-pathogenic intestinal G...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324815
更新日期:2004-05-01 00:00:00
abstract::The migration of immunocytes within the extracellular matrix (ECM) is influenced by the activation state of the incoming cell and its responses to the presence of chemokines and cytokines. We studied the regulatory role of TGF-beta1 on T cell homing to secondary lymphatic organs, such as the spleen, and chemotaxis wit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<193::AID-IMMU193>3.0
更新日期:2002-01-01 00:00:00
abstract::The mechanisms responsible for the down-modulation of the activation of separated CD4(+) or CD8(+) human T cell blasts were studied using cells obtained from healthy donors. In the presence of IL-2, human CD8(+) T cell blasts were more sensitive than CD4(+) T cell blasts to regulation by APO2 ligand/TNF-related apopto...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526046
更新日期:2005-06-01 00:00:00
abstract::In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonst...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948474
更新日期:2020-06-01 00:00:00
abstract::The causative agents of Lyme disease, Borrelia burgdorferi s.s., B. garinii, and B. afzelii, differ in their susceptibility to complement-mediated lysis. This phenomenon apparently depends on the expression of proteins termed complement regulator-acquiring surface proteins (CRASP) and their binding to the inhibitory p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323571
更新日期:2003-03-01 00:00:00
abstract::Anti-immunoglobulin antibodies (anti-Ig) provoke the rapid breakdown of phosphatidylinositol bisphosphate (PIP2), elevation of cytoplasmic Ca2+ levels ([Ca2+]i) and activation of protein kinase C (PKC) in B lymphocytes. Tumor-promoting phorbol esters, like phorbol myristate acetate, also activate PKC, but inhibit anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170119
更新日期:1987-01-01 00:00:00
abstract::NKR-P1 is a type II transmembrane protein which acts as an activation receptor on natural killer (NK) cells. The cytoplasmic domains of the CD4, CD8 and 4-1BB receptors contain the sequence Cys-X-Cys-Pro which is directly involved in coupling to another pair of cysteines in the N-terminal domain of the src family tyro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270111
更新日期:1997-01-01 00:00:00
abstract::Macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19 are members of the CC chemokine subfamily which exert their effects through specific receptors, CCR6 and CCR7, respectively. Previously, we have reported that human neutrophils have the capacity to produce a number of chemokines, including I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<1981::aid-immu19
更新日期:2001-07-01 00:00:00
abstract::With the aid of a specific rabbit antibody preparation to purified monoclonal murine IgE, two plaque-forming cell (PFC) assays have been developed for the detection and enumeration of mouse IgE-secreting cells. The first assay, utilizing protein A-coated sheep red cells (protein A-SRC), detected antibody-secreting cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101210
更新日期:1980-12-01 00:00:00
abstract::A mouse HER2-derived peptide, HER2p63 (A) (TYLPANASL), can induce K(d)-restricted mouse cytotoxic T lymphocytes (CTL) and also function as a tumor rejection antigen in an in vivo assay. Since the anchor motif of mouse K(d) for peptide binding has much similarity to that of human HLA-A2402, we asked if human HER2p63 (T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200011)30:11<3338::AID-IMMU3338>
更新日期:2000-11-01 00:00:00
abstract::Mast cells are widely distributed in tissues, particularly near surfaces exposed to the environment. Mast cells can be activated to secrete diverse mediators and cytokines by IgE and specific Ag and many other stimuli, including products derived from either pathogens or the host during innate immune responses. Althoug...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201040559
更新日期:2010-07-01 00:00:00
abstract::Precursor CD4-CD8- (DN) thymocytes rearrange their TCR-beta genes, and only those which succeed in beta-selection subsequently expand and differentiate into immature CD4+CD8+ (DP) thymocytes. The cell subsets corresponding to the successive steps of this transition can be defined in terms of CD44 and CD25 expression. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2001010)31:10<3038::aid-immu3038
更新日期:2001-10-01 00:00:00
abstract::Viruses encode immune evasion mechanisms to survive in the immunocompetent host. Chemokines mediate the migration of immune cells and their critical role in immunity is emphasized by the numerous virus-encoded strategies to modulate their activity. Evidence published in this issue of the European Journal of Immunology...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737215
更新日期:2007-04-01 00:00:00
abstract::Cold agglutinins (CA) are autoantibodies that bind to erythrocyte carbohydrates at low temperatures and induce complement-mediated cell lysis, thus causing hemolytic anemia. Tolerance mechanisms towards CA-expressing B cells and the factors inducing pathogenic CA production are unknown. In order to develop an animal m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2000)30:8<2290::AID-IMMU2290>3.0
更新日期:2000-08-01 00:00:00
abstract::Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3
更新日期:2000-07-01 00:00:00
abstract::Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunizati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050612
更新日期:1976-06-01 00:00:00
abstract::Current models of lymphocyte traffic suggest that homing specificities of T cells to tissues such as skin are generated outside the thymus as a result of activation of naive T cells by antigen in lymph nodes. Virgin T cells are thought to home to high endothelial venules in lymph nodes, but are thought to be unable to...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250314
更新日期:1995-03-01 00:00:00
abstract::Autophagy is an intracellular degradation system that plays an important role in T-cell survival. However, the precise mechanism linking autophagy and cell death in primary human T cells is unclear because methods for monitoring autophagy in small numbers of primary human cells remain controversial. We established a n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344248
更新日期:2014-08-01 00:00:00
abstract::Previous reports suggested that lethal toxin (LT)-induced caspase-1 activity and/or IL-1beta accounted for Bacillus anthracis (BA) infection lethality. In contrast, we now report that caspase-1-mediated IL-1beta expression in response to BA spores is required for anti-BA host defenses. Caspase-1(-/-) and IL-1beta(-/-)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838141
更新日期:2008-06-01 00:00:00
abstract::The genetic control of in vitro primary syngeneic sensitization on monolayers of thyroid epithelial cells was investigated both at the level of the responder lymphocytes and that of stimulator thyroid epithelial cells using congenic resistant strains of mice. It clearly appears that similar to the in vivo model of exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131115
更新日期:1983-11-01 00:00:00
abstract::Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>
更新日期:2000-12-01 00:00:00
abstract::The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171006
更新日期:1987-10-01 00:00:00
abstract::The in vitro immune response of murine spleen cells to the T cell-independent antigen, 2,4-dinitrophenyl-beta-alanyl-glycyl-glycyl-N(2-aminoethyl)carbamyl-methylated Ficoll (DAGG-Ficoll), requires the presence of macrophages, or 24-hr culture supernates from peritoneal cells. Complete reconstitution of the response by...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081105
更新日期:1978-11-01 00:00:00
abstract::Dendritic cells (DCs) are essential in dictating the nature and effectiveness of immune responses. In the intestine DCs can be separated into discrete subsets, defined by expression of CD11b and CD103, each with different developmental requirements and distinct functional potential. Recent evidence has shown that diff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747073
更新日期:2018-01-01 00:00:00
abstract::Regulatory T cells (Treg) provide protection from autoimmune disease, graft-versus-host disease, transplant rejection and overwhelming tissue destruction during infections. Conversely, high Treg numbers enable cancer cells to evade the host immune response. Thus, Treg are seen as an important tool to manipulate the im...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200636459
更新日期:2006-11-01 00:00:00