Abstract:
:Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in high concentrations of GM-CSF (GM(hi) DC) or in low GM-CSF plus IL-4 (GM(lo)/IL-4 DC). An effect of IL-4 on maturation was observed only at low but not high doses of GM-CSF. Compared to mature DC, GM(lo) DC were phenotypically immature, weak stimulators of allogeneic and peptide-specific T cell responses, but substantially more potent in presentation of native protein. Immature GM(lo) DC were resistant to maturation by lipopolysaccharide, TNF-alpha or anti-CD40 monoclonal antibodies, as the expression of co-stimulatory molecules was not increased, and stimulatory activity in oxidative mitogenesis was not enhanced. These maturation-resistant immature GM(lo) DC induced T cell unresponsiveness in vitro and in vivo. GM(lo) DC also prolonged haplotype-specific cardiac allograft survival (from 8 days to >100 days median survival time) when they were administered 7 days (but not 3, 14 or 28 days) before transplantation. Our findings may have important implications for future studies in T cell tolerance induction in vivo.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Lutz MB,Suri RM,Niimi M,Ogilvie AL,Kukutsch NA,Rössner S,Schuler G,Austyn JMdoi
10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
1813-22issue
7eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3journal_volume
30pub_type
杂志文章abstract::The effect of cyclosporin A (CsA) on the delayed-type hypersensitivity (DTH) elicited in mice by sheep red blood cells was investigated. Evidence is presented that a single injection of CsA adversely affects the inflammatory reaction. Sensitized T lymphocytes initiate the DTH reaction by their recruiting activity on p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140407
更新日期:1984-04-01 00:00:00
abstract::Mice carrying the lpr gene, SCG and MRL-lpr/lpr mice, were used to characterize the phenotype and lpr gene of abnormally proliferating T cells in these mice. A major population which expanded in these mice were T cells expressing intermediate (int) levels of T cell receptor (TCR) (and CD3) and the phenotype of interle...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260702
更新日期:1996-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220604
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
abstract::Follicular helper T (Tfh) cells provide crucial help to germinal center B (GCB) cells for proper antibody production, and a specialized subset of regulatory T cells, follicular regulatory T (Tfr) cells, modulate this process. However, Tfr-cell function in the GC is not well understood. Here, we define Tfr cells as a C...
journal_title:European journal of immunology
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doi:10.1002/eji.201546094
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journal_title:European journal of immunology
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更新日期:1998-10-01 00:00:00
abstract::Despite accounting for only a minor fraction of all cells in Hodgkin's lymphoma tissue, the Hodgkin and Reed-Sternberg (HRS) cells represent the malignant tumor cell clone in Hodgkin's disease (HD). By far the most abundant cell type in the tumor tissue are CD4+ T cells. Some of them intimately associate with HRS cell...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-08-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1998-10-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1985-01-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2002-08-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210415
更新日期:1991-04-01 00:00:00
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更新日期:2007-12-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.201747289
更新日期:2018-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3380::AID-IMM
更新日期:1999-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 评论,杂志文章
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journal_title:European journal of immunology
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doi:10.1002/eji.1830240205
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210209
更新日期:1991-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250238
更新日期:1995-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1998-10-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
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更新日期:1995-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200436
更新日期:1990-04-01 00:00:00