Abstract:
:Despite accounting for only a minor fraction of all cells in Hodgkin's lymphoma tissue, the Hodgkin and Reed-Sternberg (HRS) cells represent the malignant tumor cell clone in Hodgkin's disease (HD). By far the most abundant cell type in the tumor tissue are CD4+ T cells. Some of them intimately associate with HRS cells forming rosettes around them. This study addresses the question whether the rosetting phenomenon reflects a specific interaction between T and HRS cells by asking whether the rosettes are composed of T cells expressing a restricted TCR repertoire. Single rosetting T cells were micromanipulated from frozen sections of tumor tissue in two cases of nodular sclerosing HD and one case of lymphocyte predominant HD. TCR Vbeta gene rearrangements were amplified from these single cells by PCR. Of 83 potentially functional Vbeta gene rearrangements obtained altogether from the three cases, 81 were found to be clonally unrelated. Furthermore, they did not show signs of selection of the receptor chains for recognition of common epitopes: The usage of Vbeta and Jbeta gene segments as well as the distribution of complementarity-determining region (CDR) 3 lengths was similar to what was seen in a collection of 60 Vbeta gene rearrangements from blood of healthy donors and no recurrent CDR3 amino acid motifs were found. These data suggest that the HRS cells attract CD4+ T cells nonspecifically.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Roers A,Montesinos-Rongen M,Hansmann ML,Rajewsky K,Küppers Rdoi
10.1002/(SICI)1521-4141(199808)28:08<2424::AID-IMMsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
2424-31issue
8eissn
0014-2980issn
1521-4141pii
10.1002/(SICI)1521-4141(199808)28:08<2424::AID-IMMjournal_volume
28pub_type
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