Abstract:
:To evaluate a long peptide-based allergy vaccine in a murine model, CBA/J mice were sensitized with low dose alum-adsorbed phospholipase A2 (PLA2), a major bee venom allergen. Presensitized mice were treated by daily i.p. injections of a mixture of three long overlapping peptides (44- to 60-mer) spanning the entire PLA2 molecule (100 microg/peptide) for 6 consecutive days. This therapeutic approach induced a sharp drop in PLA2-specific IgE, an increase in specific IgG2a, and a marked T cell hyporesponsiveness. T cell cytokine secretion was characterized by a shift from a Th2 to a Th1 profile. Prophylactic treatment of naive mice with long peptides prior to sensitization with PLA2 induced a comparable modulation of B and T cell responses. Upon i.p. challenge with native PLA2, presensitized mice treated with the long peptide mixture were fully protected from anaphylaxis. This indicated that allergen-derived long overlapping peptides were safe and able to modulate an established Th2 response or to prevent its development. Furthermore, long peptide-based immunotherapy provided clinical protection against anaphylaxis, thus appearing as a promising approach of the therapy of allergic diseases.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
von Garnier C,Astori M,Kettner A,Dufour N,Heusser C,Corradin G,Spertini Fdoi
10.1002/1521-4141(200006)30:6<1638::AID-IMMU1638>3keywords:
subject
Has Abstractpub_date
2000-06-01 00:00:00pages
1638-45issue
6eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200006)30:6<1638::AID-IMMU1638>3journal_volume
30pub_type
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