Abstract:
:CD59 is a 18-20-kDa membrane glycoprotein that inhibits formation of the membrane attack complex of complement (C) on homologous cells. In the present study we analyzed the expression and function of CD59 on human endothelial cells. Immunohistochemical analysis of renal cortex demonstrated a predominant expression of CD59 on peritubular capillary endothelial cells and glomerular endothelial cells. Flow cytometry analysis showed that human umbilical vein endothelial cells (HUVEC) expressed CD59 and the fluorescence intensity was approximately four times that of peripheral blood lymphocytes. CD59 is detected on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single 20-kDa molecule in 2% deoxycholate extracts of HUVEC. CD59 was released from the surface of HUVEC by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor. The functional activity of CD59 expressed on HUVEC was studied. Blocking of CD59 antigen with F(ab')2 fragments of polyclonal anti-CD59 enhanced markedly the susceptibility of HUVEC to C-mediated lysis. This effect was dependent on the amount of blocking antibodies added. Northern blot analysis revealed the presence of three species of mRNA expressed in HUVEC, which hybridized to a cDNA probe specific for CD59, with sizes of about 800, 1400 and 2000 bp. These findings suggest that CD59 may be important in protection of endothelial cells against C-mediated damage at local sites of inflammation, thereby maintaining the vascular integrity in vivo.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Brooimans RA,Van der Ark AA,Tomita M,Van Es LA,Daha MRdoi
10.1002/eji.1830220324keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
791-7issue
3eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::Development of Th1 and Th2 effector lymphocytes is driven primarily by IL-12 or IL-4, but is also influenced by the strength of antigenic stimulation. However, the mechanism by which TCR signaling contributes to T cell polarization remains elusive. We show that in the presence of IL-12 a short TCR stimulation can lead...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4092::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::The mast cell function-associated antigen (MAFA) is a membrane glycoprotein first identified on rat mucosal type mast cells (line RBL-2H3) and known to inhibit the Fc epsilon RI-mediated secretory response. In its extracellular domain, an amino acid stretch homologous to the carbohydrate binding domain of calcium-depe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271014
更新日期:1997-10-01 00:00:00
abstract::C57BL/10ScCr (Cr) mice carry a deletion of the Toll-like receptor 4 (tlr4) gene (i.e. they are tlr4(0/0)) and are thus refractory to LPS effects. Insertion of wild-type tlr4 transgene into the tlr4(0/0) Cr germ line endowed LPS susceptibility in the two transgenic lines created, indicating that TLR4 is the only limiti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323431
更新日期:2003-03-01 00:00:00
abstract::T cell-depleted B cells from a patient with LFA-1 deficiency were tested in costimulation assays for responsiveness to recombinant human IL4 (BSF-1) and purified low molecular weight B cell growth factor (BCGFlow). In both cases the response of LFA-1-deficient B cells was comparable with normal controls. Monoclonal an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180212
更新日期:1988-02-01 00:00:00
abstract::Antigen-specific human T lymphocytes were selected by adsorption of peripheral blood mononuclear cells on monolayers of antigen-treated autologous monocytes. The enriched cell populations were propagated in interleukin 2-containing medium for 60-80 days. Their activity was tested by a proliferative response against th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131116
更新日期:1983-11-01 00:00:00
abstract::Paxillin is an adaptor protein associated with focal adhesion complex, and is activated by tyrosine phosphorylation through focal adhesion kinase (FAK) and Src kinase. Recent studies reveal that serine phosphorylation of paxillin by JNK and p38 MAPK is essential for cell migration or neurite extension, but their cellu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142192
更新日期:2012-08-01 00:00:00
abstract::A fraction of activated CD8+ T cells expresses CD40 ligand (CD40L), a molecule that plays a key role in T cell-dependent B cell stimulation. CD8+ T cell clones were examined for CD40L expression and for their capacity to allow the growth and differentiation of B cells, upon activation with immobilized anti-CD3. Accord...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251039
更新日期:1995-10-01 00:00:00
abstract::The inositol phospholipid metabolism and the increase in cytosolic free Ca2+ concentration ([Ca2+]i) into the cell are recognized as two important events in the anti-mu-induced B cell activation. The anti-mu stimulation caused the [3H]inositol incorporation and also a rapid increase in [Ca2+]i from 85 nM to 285 nM. Th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170916
更新日期:1987-09-01 00:00:00
abstract::We recently constructed IL-15 transgenic (Tg) mice using cDNA encoding a secretable isoform of the IL-15 precursor protein under the control of an MHC class I promoter. The IL-15 Tg mice exhibited resistance against a primary infection with Listeria monocytogenes. The numbers of memory CD8(+) T cells were markedly inc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200103)31:3<757::aid-immu757>3.0
更新日期:2001-03-01 00:00:00
abstract::Majority of HIV-2-infected individuals meet the criteria of long-term non-progressors. This has been linked to superior qualitative HIV-2-specific cellular immune responses that correlate with viral control. However, it is unknown whether this is due to frequent targeting of immunodominant Gag epitopes in HIV-2 than H...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838759
更新日期:2008-12-01 00:00:00
abstract::The cloned human gamma 1 heavy chain gene (HIG1) was scarcely expressed in the stable transformants of a mouse fibroblast line, L cell or a T cell line, EL4, and no gamma 1 heavy chain was produced in these cells. Upon treatment with cycloheximide or other kinds of protein synthesis inhibitors, the transcription of HI...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170906
更新日期:1987-09-01 00:00:00
abstract::The 4-1BB is a costimulatory molecule similar to the receptor activator of NF-kappaB ligand (RANKL), both of which are key factors for the differentiation of osteoclasts and are expressed mainly by activated T cells. The 4-1BB shares common signaling pathways with RANK, suggesting a potential role in osteoclastogenesi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737650
更新日期:2008-06-01 00:00:00
abstract::Vaccinia virus (VV)-encoded MHC class I Kd molecules which differ by a single amino acid change from glutamine (Kdw, wild type) to histidine (Kdm, mutant) at position 114 located in the floor of the peptide binding groove were compared in terms of peptide binding and cytotoxic T (Tc) cell recognition. Most anti-viral ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1228::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::The glycoprotein CD8 is believed to play an important role in the maturation and function of MHC class I-restricted T lymphocytes. CD8 has been proposed to function as a co-receptor of the TcR to participate in signal transduction, possibly through its cytoplasmic domain that binds to protein tyrosine kinase p56lck. A...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231117
更新日期:1993-11-01 00:00:00
abstract::Newborn mouse spleen, whose cells strongly suppress the in vitro humoral response of adult spleen cells, is essentially a hematopoietic organ. It contains a large percentage of proliferating cells, among which about 50% are erythroblasts (identified by their spectrin content) and about 15% are cells of the myelocytic ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110112
更新日期:1981-01-01 00:00:00
abstract::Gene targeting in hybridoma cells provides a tool for generating chimeric antibodies with great ease and at high yield. We present an evaluation of integration vectors for the chimerization of the immunoglobulin heavy chain locus which are universally applicable to hybridomas of different isotypes and mouse strains. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250325
更新日期:1995-03-01 00:00:00
abstract::Natural killer (NK) cells (CD56(+)/CD3(-)) in the circulation of cancer patients were reported to have low NK activity and undergo spontaneous apoptosis. A possible relationship between apoptosis and impaired NK activity was studied by Annexin V-binding and NK-cell assays performed with peripheral blood mononuclear ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200390014
更新日期:2003-01-01 00:00:00
abstract::Lipoproteins or lipopeptides (LP) are bacterial cell wall components detected by the innate immune system. For LP, it has been shown that TLR2 is the essential receptor in cellular activation. However, molecular mechanisms of LP recognition are not yet clear. We used a FLAG-labeled derivative of the synthetic lipopept...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425336
更新日期:2005-03-01 00:00:00
abstract::Treatment of systemic lupus erythematosus patients with epratuzumab (Emab), a humanized monoclonal antibody targeting CD22, leads to moderately reduced B-cell numbers but does not completely deplete B cells. Emab appears to induce immunomodulation of B cells, but the exact mode of action has not been defined. In the p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646383
更新日期:2016-09-01 00:00:00
abstract::The contribution of the costimulatory molecules B7-1 and B7-2 to the in vivo differentiation of Th cells remains controversial. The infection of resistant and susceptible strains of mice with the parasite Leishmania major provides a well-established model for studying in vivo differentiation of CD4+ T cells. We have i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1764::AID-IMMU1764>3
更新日期:2002-06-01 00:00:00
abstract::Large-scale extravasation of lymphocytes takes place in vivo under physiological conditions in lymph nodes at very specialized vascular segments called high endothelial venules (HEV). When circulating lymphocytes leave the blood, they first bind to endothelial cells of HEV (HE cells) and subsequently enter lymph nodes...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180814
更新日期:1988-08-01 00:00:00
abstract::NK cells display several killer inhibitory receptors (KIR) specific for different alleles of MHC class I molecules. A family of KIR are represented by type I transmembrane proteins belonging to the immunoglobulin superfamily (Ig-SF). Besides cDNA encoding for these KIR, additional cDNA have been identified which encod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199806)28:06<1980::AID-IMM
更新日期:1998-06-01 00:00:00
abstract::The expression of human leukocyte function-associated antigens (HLFA, equivalents of murine LFA-1) was studied on early lympho-hemopoietic cells in infant thymus and normal bone marrow by double immunofluorescence methods, cell sorting and colony-forming assays. Monoclonal antibodies MHM-24 recognizing the alpha chain...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160513
更新日期:1986-05-01 00:00:00
abstract::To study allelic exclusion of TcR genes we analyzed two types (I and II) of TcR beta transgenic mice. T cells derived from both types of mice contained similar amounts of transgenic RNA transcripts; however, surface expression of the transgenic beta chain was drastically reduced in type II compared to type I. In type ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200227
更新日期:1990-02-01 00:00:00
abstract::The role of B cells and antibody in the pathogenesis of experimental autoimmune encephalomyelitis (EAE) remains controversial. We previously demonstrated that B cells are required for EAE to be induced by the 120-amino acid extracellular domain of myelin oligodendrocyte glycoprotein (MOG). In the present study, the ro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200207)32:7<1905::AID-IMMU1905>3
更新日期:2002-07-01 00:00:00
abstract::Site-directed mutagenesis of a recombinant Fc hinge fragment has recently been used to localize the site of the mouse IgG1 (mIgG1) molecule that is involved in the intestinal transfer of recombinant Fc hinge fragments in neonatal mice. This site encompasses Ile-253, His-310, Gln-311, His-433 and Asn-434, localized at ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261038
更新日期:1996-10-01 00:00:00
abstract::Chloride intracellular channel (CLIC) 4 has diverse functions in membrane trafficking, apoptosis, angiogenesis and cell differentiation. CLIC4 is abundantly expressed in macrophages, but its role in innate immune functions is unclear. Here, we show that primary murine macrophages express increased amounts of CLIC4 aft...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041266
更新日期:2011-05-01 00:00:00
abstract::IL-23, a proximal regulator of IL-17, may be a major driving force in the induction of autoimmune inflammation. We have used a model of subdiabetogenic treatment with multiple low doses of streptozotocin (MLD-STZ; 4 x 40 mg/kg body weight) in male C57BL/6 mice to study the effect of IL-23 on immune-mediated beta cell ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535325
更新日期:2006-01-01 00:00:00
abstract::IL-7 is a major regulator of lymphocyte homeostasis; however, little is known about the mechanisms that regulate IL-7 production. To study Il7 gene regulation in vivo, we generated a novel IL-7-reporter mouse, which allows the non-invasive quantification of Il7 gene activity in live mice and, additionally, the simulta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040441
更新日期:2010-09-01 00:00:00
abstract::DNA vaccination is an efficient way to induce CD8+ T cell memory, but it is still unclear to what extent such memory responses afford protection in vivo. To study this, we induced CD8+ memory responses directed towards defined viral epitopes, using DNA vaccines encoding immunodominant MHC class I-restricted epitopes o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323928
更新日期:2003-07-01 00:00:00