Abstract:
:IL-23, a proximal regulator of IL-17, may be a major driving force in the induction of autoimmune inflammation. We have used a model of subdiabetogenic treatment with multiple low doses of streptozotocin (MLD-STZ; 4 x 40 mg/kg body weight) in male C57BL/6 mice to study the effect of IL-23 on immune-mediated beta cell damage and the development of diabetes, as evaluated by blood glucose, quantitative histology, immunohistochemistry and expression of relevant cytokines in the islets. Ten daily injections of 400 ng IL-23, starting on the first day of MLD-STZ administration led to significant and sustained hyperglycemia along with weight loss compared with controls (no IL-23), and a significant increase in the number of infiltrating cells, a lower insulin content, enhanced apoptosis, expression of IFN-gamma and IL-17 (not seen in the controls) and a significant increase in the expression of TNF-alpha and IL-18 in the pancreatic islets. IL-23 treatment started 5 days prior to MLD-STZ administration had no effect on diabetogenesis or cytokines expression in the pancreatic islets. We provide the first evidence in an animal model that IL-23 is involved in the development of type-1 diabetes, by inducing IL-17 and possibly IFN-gamma production in the target tissue.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Mensah-Brown EP,Shahin A,Al-Shamisi M,Wei X,Lukic MLdoi
10.1002/eji.200535325keywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
216-23issue
1eissn
0014-2980issn
1521-4141journal_volume
36pub_type
杂志文章abstract::C57BL/10ScCr (Cr) mice carry a deletion of the Toll-like receptor 4 (tlr4) gene (i.e. they are tlr4(0/0)) and are thus refractory to LPS effects. Insertion of wild-type tlr4 transgene into the tlr4(0/0) Cr germ line endowed LPS susceptibility in the two transgenic lines created, indicating that TLR4 is the only limiti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323431
更新日期:2003-03-01 00:00:00
abstract::CD40-CD40 ligand (CD40L) signaling plays multiple indispensable roles in cellular and humoral immunity. Impaired memory T-cell responses in the absence of CD40L have been well documented, but the requirement of this interaction for efficient priming of CD8+ T cells especially under inflammatory conditions has been und...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646420
更新日期:2016-11-01 00:00:00
abstract::Although alterations in CD3-associated signal-transducing molecules in tumor-infiltrating T cells of patients with advanced cancer have been previously described, the mechanism behind these changes is not known. We demonstrate that macrophages isolated from metastatic lymph nodes of patients with malignant melanoma do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260620
更新日期:1996-06-01 00:00:00
abstract::Biochemical and functional studies have demonstrated major histocompatibility complex (MHC) class II-restricted presentation of peptides derived from cytosolic proteins, but the underlying processing and presentation pathways have remained elusive. Here we show that endogenous presentation of an epitope derived from t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323730
更新日期:2003-05-01 00:00:00
abstract::Allergic asthma is a chronic inflammation of the airways mediated by an adaptive type 2 immune response. Upon allergen exposure, group 2 innate lymphoid cells (ILC2s) can be rapidly activated and represent an early innate source of IL-5 and IL-13. Here, we used a house dust mite (HDM)-driven asthma mouse model to stud...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546119
更新日期:2016-06-01 00:00:00
abstract::Vaccination of mice with irradiated Schistosoma mansoni larvae confers high levels of immunity which is mediated by Th1-type lymphocytes. To investigate a possible role for IL-12 in the induction of protection, we have compared the immune response of IL-12 p40-deficient (KO) mice and their C57BL/6 (WT) counterparts fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2827::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Specific members of the Rho family of GTPases exert unique influences on thymocyte proliferation, differentiation and deletion. Dbs is a guanine nucleotide exchange factor which is expressed throughout thymocyte development and is able to activate the Rho family GTPases CDC42, RhoA and RhoG. Transgenic mice expressing...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324400
更新日期:2004-03-01 00:00:00
abstract::ME3738 is a new compound that attenuates liver disease in several models of acute and chronic liver inflammation. We used the concanavalin A (Con A) model to elucidate the molecular mechanisms of ME3738 to block liver cell damage. Pretreatment of BALB/c mice with ME3738 prior to Con A injection resulted in a significa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323651
更新日期:2003-08-01 00:00:00
abstract::The purpose of this work was to study the interrelationship of proliferation and secretion of plasminogen activator (PA) and interferon (IFN) by murine macrophages. For induction of macrophage proliferation and secretion of PA, concanavalin A (Con A) was used. Secretion of IFN was induced by polyinosinic polycytidylic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130210
更新日期:1983-02-01 00:00:00
abstract::Endoglin is an homodimeric membrane antigen with capacity to bind transforming growth factor-beta (TGF-beta) and whose expression is up-regulated on myeloid cells upon differentiation to macrophages. We have isolated full-length cDNA clones from a lambda gt 10 library, prepared from phorbol 12-myristate 13-acetate-dif...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230943
更新日期:1993-09-01 00:00:00
abstract::Our understanding of the biology of human natural killer (NK) cells has significantly advanced in recent years upon identification of a family of NK cell-expressed genes that encode killer cell inhibitory receptors (KIR). Individual KIR can selectively bind various HLA class I allotypes and consequently transduce inhi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199802)28:02<599::AID-IMMU
更新日期:1998-02-01 00:00:00
abstract::Protein kinase C (PKC) has been implicated in the signaling of a number of cellular responses including activation of T cells. In the present report we have evaluated the effect of increased cAMP levels on PKC activation after stimulation of two distinct receptor systems on normal human T cells. PKC substrate phosphor...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190622
更新日期:1989-06-01 00:00:00
abstract::Low zone tolerance (LZT) to contact allergens is induced by epicutaneous exposure to haptens in subsensitizing doses resulting in an inhibition of contact hypersensitivity (CHS), which, in contrast, occurs after sensitization with immunogenic doses of allergens. Performing the protocol of tolerance induction resulted ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425402
更新日期:2004-11-01 00:00:00
abstract::Thymic nurse cells, cortical epithelial cells enclosing 20-200 lymphocytes, were prepared from mouse thymus by enzyme digestion and repetitive sedimentation. Individual nurse cells were then isolated free of any exogenous thymocytes by micromanipulation, and the endogeneous thymocytes released from inside the nurse ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151016
更新日期:1985-10-01 00:00:00
abstract::A gene-encoding ligand for deletion of T cells bearing TcRV beta 6 and V beta 8.1 cosegregates a new mammary tumor provirus locus, Mtv-50 in NC mice. The sequence of the open reading frame (ORF) in the 3' long terminal repeat (LTR) of Mtv-50 was strikingly similar to those of Mtv-7, Mtv-43 and exogenous mouse mammary ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240235
更新日期:1994-02-01 00:00:00
abstract::The thymus is the primary organ for T-cell differentiation and maturation. Unlike other major organs, the thymus is highly dynamic, capable of undergoing multiple rounds of almost complete atrophy followed by rapid restoration. The process of thymic atrophy, or involution, results in decreased thymopoiesis and emigrat...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201142305
更新日期:2012-05-01 00:00:00
abstract::Tissue distribution and expression on mitogen and virally stimulated lymphocytes render the ACT35 molecule a human lymphocyte activation antigen which as yet could not be clustered. Expression cloning of the ACT35 antigen from a pCDM8 library of the HUT-102 cell line revealed strong homology of the cDNA and its encode...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240329
更新日期:1994-03-01 00:00:00
abstract::DC not only activate CD4 T (Th) cell and cytotoxic CD8 T cell (CTL) responses against pathogens, but they also tolerize autoreactive T cells in order to avoid autoimmunity. Previous studies have demonstrated that steady-state DC can tolerize naïve CTL, naïve Th cells and memory CTL. A study in this issue of the Europe...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040704
更新日期:2010-07-01 00:00:00
abstract::Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3
更新日期:2000-07-01 00:00:00
abstract::Deficiencies in terminal complement components, including the component C7, are uncommon and associated with an increased risk of recurrent systemic neisserial infection. A total of 22 molecular defects have been reported in the C7 gene with both complete (C7Q0) and subtotal (C7SD) C7 deficiencies. In this study we re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636812
更新日期:2007-05-01 00:00:00
abstract::We have studied the kinetics of the antigen induced response of naive and memory CD8 T cells expressing a transgenic T cell receptor (TCR) specific for the glycoprotein peptide amino acid 33-41 (GP33) of the lymphocytic choriomeningitis virus (LCMV). Memory T cells were generated in vivo by adoptive transfer of LCMV T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199901)29:01<284::AID-IMMU
更新日期:1999-01-01 00:00:00
abstract::The effect of Trypanosoma brucei infections on parasite-specific antibody responses was examined by immunization of infected mice with radio-attenuated trypanosomes of a noncross-reacting clone. Antibody titers (total Ig) against variant surface antigen were determined by indirect immunofluorescence. The suppression r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101216
更新日期:1980-12-01 00:00:00
abstract::Lipoproteins or lipopeptides (LP) are bacterial cell wall components detected by the innate immune system. For LP, it has been shown that TLR2 is the essential receptor in cellular activation. However, molecular mechanisms of LP recognition are not yet clear. We used a FLAG-labeled derivative of the synthetic lipopept...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425336
更新日期:2005-03-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) is acquired by neonates through milk and first infects lymphocytes in Peyer's patches. We show here that newborn mice lacking beta7 integrin or L-selectin were infected with MMTV at wild-type levels in both their lymphoid and mammary tissues. Superantigen-mediated activation and cognat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<945::AID-IMMU945>3.0
更新日期:2002-04-01 00:00:00
abstract::DNase II in macrophages cleaves the DNA of engulfed apoptotic cells and of nuclei expelled from erythroid precursor cells. Macrophages in DNase II-deficient mice accumulate undigested DNA and constitutively produce IFN-beta as well as TNF-alpha. The IFN-beta causes severe anemia in the DNase II(-/-) embryos, which die...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838559
更新日期:2008-11-01 00:00:00
abstract::Several T cell abnormalities have been described in the course of acute Trypanosoma cruzi infection in mice, including severe effects on the thymus. In the present study, looking at the expression of extracellular matrix ligands in the thymus, we observed that deposits of fibronectin and laminin increased progressivel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323860
更新日期:2003-09-01 00:00:00
abstract::The MHC class Ib molecule H2-M3 presents N-formylated peptides from the N terminus of proteins encoded by the mitochondrial genome to CTL. A panel of CTL specific for a peptide derived from a mitochondrial protein, either COI or ND1, was used to determine the optimal peptide length for sensitizing antigen-deficient ta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3773::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::Human Vγ9Vδ2 T cells are potent anti-tumor lymphocytes that specifically respond to pyrophosphate (phospho-) antigens, which constitute the basis of current γδ T-cell-based immunotherapy strategies. Despite a clear involvement of the TCR, the costimulation requirements of Vγ9Vδ2 T cells remain ill-defined. Here, we sh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040905
更新日期:2011-01-01 00:00:00
abstract::A study was made of the ability of the superantigen staphylococcal enterotoxin B (SEB) to induce relapses of experimental allergic encephalomyelitis (EAE) in SJL mice that had partially or completely recovered from acute EAE. We find that a single injection of 0.05 mg SEB i.v. induces mild relapses in 50% of such mice...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251108
更新日期:1995-11-01 00:00:00
abstract::Orally administrated iron is suspected to increase susceptibility to enteric infections among children in infection endemic regions. Here we investigated the effect of dietary iron on the pathology and local immune responses in intestinal infection models. Mice were held on iron-deficient, normal iron, or high iron di...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545642
更新日期:2015-09-01 00:00:00