Abstract:
:Protein kinase C (PKC) has been implicated in the signaling of a number of cellular responses including activation of T cells. In the present report we have evaluated the effect of increased cAMP levels on PKC activation after stimulation of two distinct receptor systems on normal human T cells. PKC substrate phosphorylation can be induced via either the CD3 complex or, to a limited extent, the high affinity interleukin 2 (IL 2) receptor. Substrate phosphorylation via both pathways is shown to be blocked by increased intracellular levels of cAMP. In accordance with previous reports, the CD3-dependent autocrine proliferative response could also be blocked by a cAMP-dependent mechanism. Since direct activation of PKC with a phorbol ester reversed this inhibition, a causal relationship between cAMP-dependent PKC blockage and inhibition of the CD3 response is suggested. In contrast, however, initiation of IL 2-induced proliferation was essentially unaltered by cAMP and could progress in the apparent absence of PKC activity. Thus, this study indicates that IL 2-induced proliferation can under such conditions be completely uncoupled from IL 2-induced PKC activation in normal T cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Friedrich B,Cantrell DA,Gullberg Mdoi
10.1002/eji.1830190622subject
Has Abstractpub_date
1989-06-01 00:00:00pages
1111-6issue
6eissn
0014-2980issn
1521-4141journal_volume
19pub_type
杂志文章abstract::Alkaline phosphatases (APases, EC 3.1.3.1) are ecto-enzymes bound to cell membranes by a phosphatidyl-inositol anchor. We have previously shown that APase is present on activated murine B cells and its expression correlates with the process of B cell differentiation into immunoglobulin secretion. Recently, a monoclona...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200436
更新日期:1990-04-01 00:00:00
abstract::A human monoclonal IgE from patient DES, IgE (DES), has been shown to activate the classical pathway of complement. The mechanism of this activation has been investigated and can be summarized as follows: (a) IgE (DES) is able to bind and activate C1 in a dose-dependent fashion. This activation increases with the size...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140310
更新日期:1984-03-01 00:00:00
abstract::Leishmania-induced macrophage (Mphi) dysfunctions have been correlated with altered signaling events. Recent findings from our laboratory suggest that modulation of host protein tyrosine phosphatase (PTP) following Leishmania infection could lead to these Mphi defects. To address this issue, Mphi PTP activity and IFN-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199911)29:11<3737::AID-IMM
更新日期:1999-11-01 00:00:00
abstract::On the premise that an individual with an intact immune system has the capability to develop both cellular and antibody immune responses supported by the balance between the lymphokines secreted by T helper (Th) cells, we studied the interaction between different types of Th cell clones in vivo and the parameters that...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250312
更新日期:1995-03-01 00:00:00
abstract::At the surface of the murine T lymphoma cell line RMA-S, the expression of "empty" class I molecules can be dramatically enhanced by culture at 26 degrees C. These class I molecules are unstable following transfer to 37 degrees C unless they are loaded with exogenously added peptides. Class I heterodimers that have fa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220639
更新日期:1992-06-01 00:00:00
abstract::This study describes a general strategy to produce hybrid monoclonal antibodies that are capable of targeting human cytotoxic T lymphocytes (CTL) against any cell carrying the appropriate target antigen. This is done by fusing a HAT-sensitive, G418-resistant anti-T3 hybridoma with immune spleen cells (or with other hy...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170118
更新日期:1987-01-01 00:00:00
abstract::Naive and memory CD4 T cells differ in cell surface phenotype, function, activation requirements, and modes of regulation. To investigate the molecular bases for the dichotomies between naive and memory CD4 T cells and to understand how the T cell receptor (TCR) directs diverse functional outcomes, we investigated pro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270838
更新日期:1997-08-01 00:00:00
abstract::The effect of phorbol 12-myristate 13-acetate (PMA) on the synthesis, assembly and processing of the components of the T cell receptor (TcR) was studied with special focus on the CD3 omega chain. Treatment of the human leukemic T cell line Jurkat with PMA increased the synthesis of the Ti alpha, CD3 gamma and CG3 zeta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230625
更新日期:1993-06-01 00:00:00
abstract::Human cytomegalovirus (CMV) infection can be life threatening in the immune compromised and is associated with congenital defects and / or mental retardation in the neonate. The demonstrated association between CMV infection and rheumatoid factor (RF) raised the possibility of an induction of an autoimmune response up...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199911)29:11<3643::AID-IMM
更新日期:1999-11-01 00:00:00
abstract::The anti-PC antibodies of BALB/c origin bear predominantly the idiotype characteristic of the phosphorylcholine (PC)-binding T15 idiotype than sera from adult mice, and, unlike the latter, they also contain detectable amounts of anti-T15 antibodies. By 2 weeks of age the anti-T15 antibodies are no longer detectable wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060710
更新日期:1976-07-01 00:00:00
abstract::C57BL/10ScCr (Cr) mice carry a deletion of the Toll-like receptor 4 (tlr4) gene (i.e. they are tlr4(0/0)) and are thus refractory to LPS effects. Insertion of wild-type tlr4 transgene into the tlr4(0/0) Cr germ line endowed LPS susceptibility in the two transgenic lines created, indicating that TLR4 is the only limiti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323431
更新日期:2003-03-01 00:00:00
abstract::Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3
更新日期:2000-07-01 00:00:00
abstract::The multimeric Fc gamma RIIIA (CD16) complex is expressed on the surface of natural killer (NK) cells and is composed of a 50-70-kDa transmembrane glycoprotein Fc gamma receptor (CD16), the T cell receptor (TCR)-zeta chain, and the Fc epsilon RI gamma chain. Cross-linking Fc gamma RIIIA initiates the rapid tyrosine ph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231017
更新日期:1993-10-01 00:00:00
abstract::Transcriptional up-regulation of the VCAM-1 gene, induced by proinflammatory cytokines such as IL-1beta and TNF-alpha, requires activation of not only NF-kappaB, but also involves interferon regulatory factor (IRF)-1. During a study of gene induction by mechanical stimuli in cultured human endothelial cells, we noted ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200207)32:7<1821::AID-IMMU1821>3
更新日期:2002-07-01 00:00:00
abstract::Changes in the migration pattern of lymphocytes represent a key event in the evolution of an immune response since they enable lymphocytes to gain access to infected tissues. We studied the location of virus-specific CD8 T cells in various splenic compartments in response to infection with lymphocytic choriomeningitis...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199911)29:11<3562::AID-IMM
更新日期:1999-11-01 00:00:00
abstract::Beta1 integrins can provide T cell co-stimulation, but little is known concerning their downstream signaling pathways. We found that Pyk2, a focal adhesion kinase-related tyrosine kinase, is regulated by beta1 integrin signaling in human T cells. Stimulation of Jurkat T cells with the alpha4beta1 integrin ligand VCAM-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3867::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::Considering the role of interleukin-8 (IL-8) in a large number of acute and chronic inflammatory diseases, the regulation of IL-8-mediated biological responses is important. Alpha-melanocyte-stimulating hormone (alpha-MSH), a tridecapeptide, inhibits most forms of inflammation by an unknown mechanism. In the present s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535209
更新日期:2006-03-01 00:00:00
abstract::Bispecific antibodies binding to the TCR/CD3 complex and to a tumor-associated surface molecule can be used to target cytotoxic T lymphocytes against tumor cells. We reasoned that high-affinity binding to CD3 may reduce the efficiency of T cell stimulation and target the bispecific reagent to T cells rather than to tu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200211)32:11<3102::AID-IMMU3102>
更新日期:2002-11-01 00:00:00
abstract::Graft-versus-host disease (GVHD) caused by donor T cells attacking recipient tissues is a major cause of morbidity and mortality following allogeneic hematopoietic stem cell transplantation (alloSCT). Studies have shown that effector memory T (T(EM) ) cells do not cause GVHD but are capable of immune functions post-tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141678
更新日期:2011-09-01 00:00:00
abstract::The cell surface expression and biosynthesis of Langerhans cells (LC)-derived major histocompatibility complex (MHC) class II molecules from epidermal cells (EC) prepared freshly and cultured for up to 3 days was investigated. Based on the constitutive expression of MHC class II determinants by LC, a panning and magne...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210518
更新日期:1991-05-01 00:00:00
abstract::E-selectin is a cytokine-inducible membrane glycoprotein capable of mediating adhesion of leukocytes to endothelial cells. It is highly glycosylated, containing 11 sites for N-linked glycosylation. N-Glycosylation of E-selectin was analyzed by endoglycosidase treatment. Analysis of immunoprecipitated E-selectin from h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250907
更新日期:1995-09-01 00:00:00
abstract::Although potentially autoreactive T cells are present even in healthy subjects, most individuals do not develop autoimmune disease. It has been well demonstrated that CD4+ CD25+ regulatory T cells play a significant role in controlling the expansion of autoreactive T cells in the periphery. However, some healthy indiv...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636383
更新日期:2007-01-01 00:00:00
abstract::The inheritance of responsiveness to lipopolysaccharide (LPS), and of a marker recognized in LPS-reactive cells by a heterologous antiserum, was studied in crosses between C3H/HeJ (nonresponder) and C3H/Tif (high responder) mice. F1 hybrid mice show codominant expression of these traits: (a) LPS-reactive cells are onl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080113
更新日期:1978-01-01 00:00:00
abstract::The MHC-related 1 (MR1) protein is a monomorphic, evolutionarily conserved MHC class I-like molecule, which is necessary for the development and functions of mucosal-associated invariant T (MAIT) cells, a new subset of innate-like lymphocytes. Multiple isoforms of the MR1 gene are naturally transcribed, but only the f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242461
更新日期:2013-05-01 00:00:00
abstract::In this study we have investigated the specificity of bioassays in which interleukin (IL) 1 and/or IL 6 are active. The thymocyte assay cannot be used to discriminate between IL 1 and IL 6; both monokines are active in this assay. Moreover the detection limit for both IL 1 and IL 6 is around 100 pg/ml. IL 6 activity c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181010
更新日期:1988-10-01 00:00:00
abstract::The autoimmune response in experimental autoimmune thyroiditis (EAT) is characterized by a lymphocyte infiltration of the thyroid gland and by the appearance of circulating autoantibodies to thyroglobulin (Tg). Cytokines play a crucial role in the immunoregulation and pathology of EAT. Systemic administration of IL-10...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199903)29:03<958::AID-IMMU
更新日期:1999-03-01 00:00:00
abstract::Allogeneic bone marrow transplantation in the neonate is an effective way of inducing permanent tolerance to donor tissue. To do the same in the immunocompetent adult requires immunosuppression to counter host-versus-graft alloreactivity. Conditioning with monoclonal antibodies (mAb) to CD4 and CD8 has been sufficient...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221111
更新日期:1992-11-01 00:00:00
abstract::The invariant chain (Ii) binds to newly synthesized major histocompatibility complex (MHC) class II molecules and is targeted to an acidic compartment where it is degraded. To evaluate its role on the conformation and the subcellular distribution of murine MHC class II molecules we have established stable L cell trans...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231218
更新日期:1993-12-01 00:00:00
abstract::The third component of complement C3 has been implied in the stimulation of B lymphocytes to proliferation and maturation for Ig secretion. We have reinvestigated the extent of this activation with either activated or resting murine splenic lymphocytes in serum-substituted cultures. Human C3 was used in either soluble...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150214
更新日期:1985-02-01 00:00:00
abstract::Previous studies have demonstrated that immunization of BALB/c mice with alpha(1----3) dextran (Dex) is accompanied by a reduction in the subsequent immune response to the acetylcholine receptor (AChR), depending on the timing of the Dex administration relative to AChR challenge. Here, we report that suppression of th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200803
更新日期:1990-08-01 00:00:00