Abstract:
:Although potentially autoreactive T cells are present even in healthy subjects, most individuals do not develop autoimmune disease. It has been well demonstrated that CD4+ CD25+ regulatory T cells play a significant role in controlling the expansion of autoreactive T cells in the periphery. However, some healthy individuals exhibit measurable responses to self peptide even in the presence of CD4+ CD25+ regulatory cells. This article describes the regulation of human CD4+ T cell responses to glutamic acid decarboxylase 65 (GAD65), an autoantigen implicated in type-1 diabetes, by autologous CD8+ suppressor T cells. In cells cultured from healthy individuals, the inclusion of autologous CD8+ T cells at physiological levels resulted in a dramatic decrease in the magnitude of in vitro CD4+ T cell responses to GAD65 peptide. Based on transwell experiments, the observed suppression was cell contact-dependent. However, antibody blocking studies indicated that suppression was mediated by IL-10. Cell fractionation studies suggested that CD8+ suppressor T cells originate from the CD45RA+ CD27- population. The suppression of CD4+ T cell responses to GAD65 in healthy individuals raises the possibility that CD8+ suppressor T cells play an important role in controlling potentially autoreactive T cells in the general population.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
James EA,Kwok WWdoi
10.1002/eji.200636383subject
Has Abstractpub_date
2007-01-01 00:00:00pages
78-86issue
1eissn
0014-2980issn
1521-4141journal_volume
37pub_type
杂志文章abstract::Antigen-induced stimulation of T cells is mediated via the CD3 antigen receptor (Ti) complex and monoclonal antibodies (mAb) reacting with CD3 and Ti result in rapid intracellular Ca2+ mobilization, followed by monocyte-dependent proliferation. Combinations of mAb to CD2, the sheep red blood cell receptor, also mobili...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160521
更新日期:1986-05-01 00:00:00
abstract::Toll-like receptors (TLRs) play a major part in providing innate immunity against pathogenic microorganisms. Recent studies show that these receptors are also expressed on T cells, which are the sentinels of adaptive immunity. Here, we have investigated the regulatory role of the T-cell receptor in the functioning of ...
journal_title:European journal of immunology
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更新日期:2015-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2002-07-01 00:00:00
abstract::The existence of a functional receptor for secretory component (SC) on the eosinophil membrane might explain the preferential degranulation induced by secretory IgA (sIgA) when compared to serum IgA. Indeed, flow cytometry analysis revealed that purified human SC could bind to a subpopulation (4-59%) of blood eosinoph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250121
更新日期:1995-01-01 00:00:00
abstract::The conditions under which tumor necrosis factor-alpha (TNF) induces apoptosis in primary microvascular endothelial cells (MVEC) were investigated. In the absence of sensitizing agents, TNF induced apoptosis after 3 days of incubation in confluent MVEC. In contrast, upon addition of the transcriptional inhibitor actin...
journal_title:European journal of immunology
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更新日期:1998-11-01 00:00:00
abstract::H-2 gene-dependent nonresponsiveness and responsiveness to H-2-matched male cells can be observed in cytotoxic assays in several mouse strains. H-2 genes of several low responder strains can complement each other in cis or trans position to produce high responders. One of the H-2 gene products functions at the level o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070708
更新日期:1977-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101210
更新日期:1980-12-01 00:00:00
abstract::A detailed restriction map of a 430-kb contig comprising the single Ckappa, the 5 Jkappa and the adjoining 22 Vkappa gene segments is presented. The first 12 Vkappa genes following the JkappaCkappa region belong to the Vkappa21 family, the subsequent ones to the closely related families Vkappa8 and Vkappal 9/ 28. Prev...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199805)28:05<1458::AID-IMM
更新日期:1998-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200838181
更新日期:2008-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241231
更新日期:1994-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200232
更新日期:1990-02-01 00:00:00
abstract::To further define DNA immunization as a model for anti-DNA production, we investigated the cellular requirements for this response in mice immunized with single-stranded DNA from E. coli. The anti-DNA responses of genetically immune-deficient mice and congenic controls were measured by ELISA after immunization with E....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200825
更新日期:1990-08-01 00:00:00
abstract::From human precursor B cells which had both immunoglobulin (Ig) heavy (H) chain loci in germ-line configuration, various IgH chain germ-line transcripts were isolated and sequenced. These transcripts were shown to contain sequences derived from the JH region, the IgH chain enhancer element or the Ig switch region. A n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250142
更新日期:1995-01-01 00:00:00
abstract::Previously we have reported on the production of two sets of human monoclonal antibodies reacting with mouse mammary tumor virus (MMTV) and human mammary tumor virus (HuMTV) cross-reacting antigens. B11 is a monoclonal IgG generated by the human-human hybridoma technique using axillary lymph node of breast cancer pati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181109
更新日期:1988-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081103
更新日期:1978-11-01 00:00:00
abstract::The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636764
更新日期:2007-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200724
更新日期:1990-07-01 00:00:00
abstract::CD11a/CD18 (beta2)-integrins are expressed on leukocytes and are involved in cell adhesion and signaling. Despite extensive studies the signaling pathways and molecular mechanisms involved in integrin regulation in T cells remain not completely understood. We have now studied the involvement of the tyrosine kinase Lck...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200206)32:6<1670::AID-IMMU1670>3
更新日期:2002-06-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.200737518
更新日期:2008-03-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250834
更新日期:1995-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230805
更新日期:1993-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221011
更新日期:1992-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240512
更新日期:1994-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
abstract::Autophagy is an intracellular degradation system that plays an important role in T-cell survival. However, the precise mechanism linking autophagy and cell death in primary human T cells is unclear because methods for monitoring autophagy in small numbers of primary human cells remain controversial. We established a n...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201022
更新日期:1990-10-01 00:00:00