CD2 and CD3 antigens mobilize Ca2+ independently.

Abstract:

:Antigen-induced stimulation of T cells is mediated via the CD3 antigen receptor (Ti) complex and monoclonal antibodies (mAb) reacting with CD3 and Ti result in rapid intracellular Ca2+ mobilization, followed by monocyte-dependent proliferation. Combinations of mAb to CD2, the sheep red blood cell receptor, also mobilize calcium and induce mitogenesis and purified phytohemagglutinin (PHA) stimulates T cells predominantly by interaction with this molecule. It has been suggested that activation via CD2 requires the presence of CD3 and that the hydrophobic epsilon chain of CD3 is the T cell calcium channel. To investigate this further we have obtained large numbers of natural killer (NK) cells which express CD2 but not CD3 from a patient with a chronic expansion of this lymphocyte subpopulation. It is shown that calcium mobilization can be induced in these cells by mAb to CD2 and purified PHA but not by anti-CD3 mAb. This indicates that calcium mobilization can be induced via the CD2 molecule in NK cells not expressing CD3 and that activation through CD2 is separate from the antigen receptor CD3 pathway.

journal_name

Eur J Immunol

authors

O'Flynn K,Knott LJ,Russul-Saib M,Abdul-Gaffar R,Morgan G,Beverley PC,Linch DC

doi

10.1002/eji.1830160521

subject

Has Abstract

pub_date

1986-05-01 00:00:00

pages

580-4

issue

5

eissn

0014-2980

issn

1521-4141

journal_volume

16

pub_type

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