Abstract:
:The conditions under which tumor necrosis factor-alpha (TNF) induces apoptosis in primary microvascular endothelial cells (MVEC) were investigated. In the absence of sensitizing agents, TNF induced apoptosis after 3 days of incubation in confluent MVEC. In contrast, upon addition of the transcriptional inhibitor actinomycin D (Act. D), confluence was no longer required and apoptosis occurred already after 16 h. To assess the role of either TNF receptor (TNFR) type in apoptosis, MVEC isolated from mice genetically deficient in TNFR1 (Tnfr1o mice) or TNFR2 (Tnfr2o mice) were incubated with TNF in the presence or absence of Act. D. Under sensitized conditions, Tnfr2o MVEC were lysed like controls, whereas Tnfr1o MVEC were completely resistant, indicating an exclusive role for TNFR1. In contrast, in the absence of Act. D, confluent monolayers of wild-type cells were lysed by TNF, but both Tnfr1o and Tnfr2o MVEC were resistant to TNF-mediated toxicity, indicating a requirement for both TNFR types. Overexpression of the anti-apoptotic protein bcl-xL in MVEC led to a protection against the direct, but not the sensitized cytotoxicity of TNF. In conclusion, in pathophysiologically relevant conditions, both TNFR appear to be required for TNF-induced apoptosis in MVEC.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Lucas R,Garcia I,Donati YR,Hribar M,Mandriota SJ,Giroud C,Buurman WA,Fransen L,Suter PM,Nunez G,Pepper MS,Grau GEdoi
10.1002/(SICI)1521-4141(199811)28:11<3577::AID-IMMsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
3577-86issue
11eissn
0014-2980issn
1521-4141journal_volume
28pub_type
杂志文章abstract::Upon antigen-specific or allogeneic activation, T cells sharply increase their metabolic activity to cope with augmented needs for proliferation and effector functions. Therefore, enzymes involved in energy metabolism constitute attractive targets to modulate the activity of pathogenic effector T cells in the setting ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546152
更新日期:2016-09-01 00:00:00
abstract::Recent evidence suggests a role for phosphatidylinositol (PI) 3-kinase in various inflammatory responses. In this study, the consequences of LPS-induced PI 3-kinase activation on cytokine and chemokine expression and the intracellular mechanisms of inflammatory activation were examined in mouse macrophages. LPS stimul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323376
更新日期:2003-03-01 00:00:00
abstract::The active role of chemokines in the central nervous system (CNS) during the pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been clearly established. In this study the expression pattern of several chemokines and cytokines was elucidated using reverse transcription-PCR and immunohistochemistry in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1911::AID-IMMU1911>3
更新日期:2000-07-01 00:00:00
abstract::The nonobese diabetic (NOD) mouse is a relevant model for studying human insulin-dependent diabetes mellitus (IDDM). The selective destruction of insulin-secreting cells in this model is subsequent to an autoimmune reaction directed towards the beta cells inside the islets of Langerhans of the pancreas. Given the key ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210511
更新日期:1991-05-01 00:00:00
abstract::We have characterized the peripheral B cell repertoire in T cell-mediated insulin-dependent diabetes mellitus (IDMM) and in B cell-mediated autoimmune idiopathic thrombocytopenic purpura (AITP). The VH6-containing repertoire in adult patients with IDDM or AITP and healthy control subjects was investigated by PCR ampli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199909)29:09<2853::AID-IMM
更新日期:1999-09-01 00:00:00
abstract::Naturally activated T lymphocytes present in normal mouse spleen were studied for direct reactivity to interleukin 2 (IL 2) and for binding of anti-IL 2 receptor (IL 2R) antibodies or radiolabeled IL 2. The majority of large-sized splenic T lymphocytes are IL 2R-; thus, at the most one third of large L3T4+ T cells and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170702
更新日期:1987-07-01 00:00:00
abstract::In humans and in BALB/c mice, immune responses to the hormone insulin use evolutionarily related VHV (human) and VHIX (murine) gene families. To determine if these structural relationships include regulatory elements, BALB/c mice were pretreated with autologous immunoglobulin G (IgG) monoclonal antibodies (mAb) that r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220938
更新日期:1992-09-01 00:00:00
abstract::In the frog Xenopus, MHC class I antigen presentation and processing genes (the immunoproteasome LMP2 and LMP7 and the transporter TAP1 and TAP2) seem to be closely linked in a primordial organization. Two distinct lineages of class Ia and LMP7 loci were previously identified, thus strongly suggesting co-evolution amo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324207
更新日期:2003-11-01 00:00:00
abstract::Helicobacter pylori is a Gram-negative bacterium that causes a variety of gastrointestinal diseases, such as duodenal ulcer and gastric carcinoma. The T cell response against H. pylori is thought to contribute to the pathogenesis of these diseases. Here, we show that mouse-adapted H. pylori is able to polyclonally act...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636676
更新日期:2007-07-01 00:00:00
abstract::B cells and regulatory T (Treg) cells can both facilitate remission from experimental auto immune encephalomyelitis (EAE), a disease of the central nervous system (CNS) used as a model for multiple sclerosis (MS). Considering that B-cell-depletion therapy (BCDT) is used to treat MS patients, we asked whether Treg-cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142242
更新日期:2012-05-01 00:00:00
abstract::Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtain...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142343
更新日期:2013-01-01 00:00:00
abstract::Just over a decade ago, it was established that NK cells in the thymus do not follow precisely the same developmental pathway as conventional NK cells that develop in the bone marrow. Subsequently, it has emerged that NK cells are one branch of a family of innate lymphoid cells (ILCs). ILC1s and thymic NK cells have, ...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201747038
更新日期:2017-05-01 00:00:00
abstract::Multiple myeloma is a fatal B cell malignancy characterized by the accumulation of plasma cells within the bone marrow. IL-6 is a major survival factor for myeloma cells. Bcl-2 protein family regulates pathways to apoptosis that are activated upon growth factor deprivation. Pro-apoptotic proteins that have only a sing...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424981
更新日期:2004-11-01 00:00:00
abstract::Proliferation of BCL1 cells, generally used for the measurement of murine interleukin (IL)5, is shown to be strongly suppressed by interferon (IFN)-gamma. This effect can be abrogated by the addition of anti-IFN-gamma antibodies. Thus, the IL5 activity of culture supernatants derived from T cells simultaneously produc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190726
更新日期:1989-07-01 00:00:00
abstract::Macrophages provide a first line of defense against bacterial infection by engulfing and killing invading bacteria, but intracellular bacteria such as Listeria monocytogenes (LM) can survive in macrophages by various mechanisms of evasion. Complement receptor of the immunoglobulin (CRIg), a C3b receptor, binds to C3b ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242997
更新日期:2013-03-01 00:00:00
abstract::Monoclonal gammopathies (MG) are monoclonal proliferative disorders of B cells at the differentiation stage of Ig production. They can be detected in the serum, either as transient or as persistent homogenous immunoglobulin (H-Ig) components. The exact phenotype, localization, and cell lineage origin of the precursor ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270943
更新日期:1997-09-01 00:00:00
abstract::Although originally described in tumor-bearing hosts, myeloid-derived suppressor cells (MDSC) have been detected under numerous pathological situations that cause enhanced demand of myeloid cells. Thus, MDSC might be part of a conserved response to different endogenous and exogenous stress signals, including inflammat...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200939892
更新日期:2009-10-01 00:00:00
abstract::B1 cells are a subset of B lymphocytes found in many species and are implicated in the development of autoimmunity. B1 cells have previously been shown to be suppressed by the T helper (Th)1 cytokine interferon (IFN)-gamma, and to be stimulated by the Th2 cytokines interleukin (IL)-2, IL-4, IL-5 and IL-10. To examine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260134
更新日期:1996-01-01 00:00:00
abstract::Dendritic cells (DC) play an essential role in the induction of primary immune responses; however, very little information is available on cytokine production by DC. Here we determined the cytokine gene expression profile of two immortalized DC clones, CB1 and D2SC/1, both generated from mouse spleen but differing in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241039
更新日期:1994-10-01 00:00:00
abstract::The relative complement-mediated lytic capability of the IgG subclass isotypes was studied using a matched set of mouse-human chimeric anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies. The subclass pattern was shown to be highly dependent on variations in antigen concentration and to lesser extent on variation in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210103
更新日期:1991-01-01 00:00:00
abstract::A panel of closely and distantly related lysozymes and lysozyme-peptide fragments were utilized in assessing the specificity repertoire of murine anti-hen egg white lysozyme hybridomas. The 44 monoclonal antibodies could be divided into a minimum of 18 fine specificity groups in tests using the lysozyme panel. Two hyb...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140116
更新日期:1984-01-01 00:00:00
abstract::Naive and memory CD4 T cells differ in cell surface phenotype, function, activation requirements, and modes of regulation. To investigate the molecular bases for the dichotomies between naive and memory CD4 T cells and to understand how the T cell receptor (TCR) directs diverse functional outcomes, we investigated pro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270838
更新日期:1997-08-01 00:00:00
abstract::The present report describes functional characteristics of a monoclonal antibody (mAb), TA/211, which is reactive with a common determinant of the human CD45 molecules. When added to purified testing human T cells, TA/211, which is itself not mitogenic, leads to a strong proliferative response in combination with subm...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190226
更新日期:1989-02-01 00:00:00
abstract::In June 2009, the National Institute of Allergy and Infectious Diseases (NIAID), Division of Allergy, Immunology and Transplantation (DAIT), sponsored a workshop entitled Mast Cells in Innate and Adaptive Immunity. International experts in mast cell biology discussed recent advances in the field and future areas of re...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.200990325
更新日期:2010-01-01 00:00:00
abstract::Ptak and Askenase showed that both alphabeta and gammadelta cells are required for transfer of contact sensitivity (CS). This study confirms that day 4 immune cells depleted of gammadelta cells fail to transfer CS to trinitrochlorobenzene (TNP-Cl) systemically and demonstrates that administration of anti-gammadelta mo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270131
更新日期:1997-01-01 00:00:00
abstract::We and others have demonstrated that adipose tissue is a reservoir for HIV. Evaluation of the mechanisms responsible for viral persistence may lead to ways of reducing these reservoirs. Here, we evaluated the immune characteristics of adipose tissue in HIV-infected patients receiving antiretroviral therapy (ART) and i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747060
更新日期:2017-12-01 00:00:00
abstract::The covalent binding reaction of the third component of complement (C3) with rabbit IgG immune aggregates has been studied by enzymic digestion of C3b-IgG adducts. In these adducts C3b was radioactively labeled in the free thiol group generated during activation of the internal thioester of C3. Trypsin digestion of 14...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240316
更新日期:1994-03-01 00:00:00
abstract::Intravenous immunoglobulins (IVIgs) efficiently suppress a variety of autoimmune diseases. Over the past few years several potential mechanisms underlying this antiinflammatory activity have become apparent. Among these, terminal sialic acid residues in the sugar moiety of the immunoglobulin G constant fragment have b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242710
更新日期:2012-12-01 00:00:00
abstract::An immune response (Ir) gene is described which controls the ability of mice to respond to seven very closely related gallinaceous egg white lysozymes (GEL). This Ir-GEL gene locus is linked to the major histocompatibility locus of the mouse and operates at the level of the T cell. Responsiveness to the nonimmunogenic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050506
更新日期:1975-05-01 00:00:00
abstract::Traumatic brain injury (TBI) elicits innate inflammatory responses that can lead to secondary brain injury. To better understand the mechanisms involved in TBI-induced inflammation, we examined the nature of macrophages responding to TBI in mice. In this model, brain macrophages were increased >20-fold the day after i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201243084
更新日期:2013-08-01 00:00:00