Abstract:
:We have characterized the peripheral B cell repertoire in T cell-mediated insulin-dependent diabetes mellitus (IDMM) and in B cell-mediated autoimmune idiopathic thrombocytopenic purpura (AITP). The VH6-containing repertoire in adult patients with IDDM or AITP and healthy control subjects was investigated by PCR amplification using VH6- and JH-specific primers. Nucleotide sequence analysis of VH6-D-JH rearrangements showed an abnormally high frequency of somatic mutations in non-functional rearrangements from diabetic (3. 58 %) as well as AITP patients (3.18 %), compared to controls (0.4 % and 1.43 %, respectively; p < 0.05). In contrast, the mutation frequency among functional rearrangements was 2.4 - 3 times lower in patients compared to controls ( p < 0.05). Detailed analysis of the VH6 genes carrying mutations showed that the underlying mechanism for this observation is probably different for the two diseases. Analysis of D- and JH gene usage revealed additional deviations from the normal pattern. Taken together, these results suggest defects in the mechanisms controlling selection of the B cell repertoire in patients with IDDM or AITP.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Söderström I,van Dijk-Härd I,Feld S,Hillörn V,Holmberg D,Lundkvist Idoi
10.1002/(SICI)1521-4141(199909)29:09<2853::AID-IMMkeywords:
subject
Has Abstractpub_date
1999-09-01 00:00:00pages
2853-62issue
9eissn
0014-2980issn
1521-4141journal_volume
29pub_type
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