Abstract:
:A monoclonal antibody (mAb), 2D1(IgM), was identified for its anti-proliferative effect on human T leukemia cell line, SUP-T13. The cells bound with 2D1 showed DNA ladder patterns of oligonucleosomes, demonstrating apoptosis. Peripheral mononuclear cells activated by phytohemagglutinin or OKT3 induced expression of 2D1 antigen and were growth-inhibited by the antibodies. Among the cell lines tested, T cell lines tended to be growth-inhibited by the antibodies. Epstein-Barr virus-transformed B cells were reactive with 2D1, but were not growth-inhibited by the antibodies. We established stable 2D1-resistant variants LAC2D1R and JKT2D1R from the original SUP-T13 and Jurkat T cell lines, respectively. These variant cells demonstrated phenotypes identical to the original cells, including reactivity to 2D1 and expression of cytoplasmic Bc1-2 protein. The 2D1-resistant cells were as sensitive as the original cells to the other apoptosis-inducing stimuli, such as gamma-irradiation or calcium ionophore A23187. However, the 2D1-resistant variants were also insensitive to anti-Fas, another apoptosis-inducing mAb. Binding of 2D1 was blocked by anti-Fas mAb, suggesting that 2D1 reacts with an epitope of human Fas molecules. The present results demonstrate that a 2D1-reactive, but not 2D1-sensitive, population may exist in highly 2D1-sensitive human leukemia T cells and that pairs of 2D1-sensitive and 2D1-resistant cells are useful in the biochemical analysis of Fas-mediated apoptosis in human T cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Takahashi S,Sato N,Takayama S,Ichimiya S,Satoh M,Hyakumachi N,Kikuchi Kdoi
10.1002/eji.1830230831subject
Has Abstractpub_date
1993-08-01 00:00:00pages
1935-41issue
8eissn
0014-2980issn
1521-4141journal_volume
23pub_type
杂志文章abstract::The NLRP3 inflammasome is a molecular complex that translates signals from pathogens and tissue damage into inflammatory responses, and plays crucial roles in numerous neurological diseases. Activation of the NLRP3 inflammasome leads to caspase-1 dependent cleavage of pro-IL-1β to form mature IL-1β. By acting on the P...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848013
更新日期:2020-02-01 00:00:00
abstract::cDNA of the T cell receptor beta (TCRB) have been isolated from the anuran amphibian Xenopus and they show strong structural homology to TCRB sequences of other vertebrates. Ten BV families, two D segments, ten J segments, and a single C region have been defined so far. Each V family consists of one to two members per...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270327
更新日期:1997-03-01 00:00:00
abstract::Neutrophils express pattern recognition receptors (PRRs) and regulate immune responses via PRR-dependent cytokine production. An emerging theme is that neutrophil PRRs often exhibit cell type-specific adaptations in their signalling pathways. This prompted us to examine inflammasome signalling by the PRR NLRP3 in muri...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545943
更新日期:2016-04-01 00:00:00
abstract::The effect of anti-Ia antibody on the development of murine neonatal self-Ia-reactive T lymphocytes in the thymus was studied. A C57BL/6 (B6) mouse pregnant with (B6 X C3H/He)F1 (B6C3F1) embryos was injected with monoclonal anti-I-Ek (m alpha I-Ek) antibody every other day starting from the 12th day of gestation. The ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160719
更新日期:1986-07-01 00:00:00
abstract::Mice carrying the H-2b and H-2s haplotypes are genetically nonresponsive to hen egg-white lysozyme (HEL). Analysis of the anti-HEL response patterns of F1, F2 and backcross progeny showed that responsiveness was dominant and H-2 linked. From plaque-forming cell and serum assays in intra-H-2 recombinant mice, it was es...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100513
更新日期:1980-05-01 00:00:00
abstract::Antigens in the form of immune complexes are retained on the membranes of follicular dendritic cells (FDC) for long periods of time. To examine how immune complexes reach germinal centers, where FDC are located, we injected mice with anti-2,4-dinitrophenyl (DNP) antibodies complexed to DNP-myoglobin-coated gold partic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160211
更新日期:1986-02-01 00:00:00
abstract::The adoptive transfer of CD4(+)CD25(+) natural regulatory T cells (Treg) is a promising strategy for the treatment of autoimmune diseases and the prevention of alloresponses after transplantation. Clinical trials exploring this strategy require efficient in vitro expansion of this rare cell population. Protocols devel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838904
更新日期:2009-04-01 00:00:00
abstract::The thymus is a primary lymphoid organ supporting the development of self-tolerant T cells. Key events in T-cell development in the thymus include lineage commitment, selection events, and thymic emigration. This review discusses the proposed role of sphingosine-1-phosphate and its receptors in the emigration of both ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838912
更新日期:2009-04-01 00:00:00
abstract::In the bone marrow, the passage of developing B cells through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636569
更新日期:2007-04-01 00:00:00
abstract::A method is described for achieving the efficient production of proteins of biological interest by the establishment of hybridomas from lymphocytes of transgenic animals carrying a fusion gene having promoter-regulatory sequences functional in lymphocytes fused to the coding sequences of the protein to be produced. Wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160829
更新日期:1986-08-01 00:00:00
abstract::Intrathymic deletion of immature thymocytes that express self-reactive TCR specificities is essential in the generation of self tolerance. Medullary thymic epithelial cells (mTEC) expressing the transcriptional regulator Aire play a key role in this process by regulating expression of tissue-restricted antigens to ens...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200738052
更新日期:2008-04-01 00:00:00
abstract::The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324336
更新日期:2004-02-01 00:00:00
abstract::Peritoneal macrophages from C57BL/6 mice process antigens from bacteria or coated on polystyrene beads for presentation by major histocompatibility complex (MHC) class I molecules. To investigate this antigen processing pathway, peritoneal macrophages from homozygous TAP1-/- mice, which lack the transporter associated...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261135
更新日期:1996-11-01 00:00:00
abstract::The interaction of CD40 ligand (CD40L) on activated T cells with CD40 on B cells, monocytes and dendritic cells is essential for humoral immunity and for up-regulation of antigen-presenting cell (APC) functions, as a result of signaling through CD40. There are also some indications that after interaction with CD40, CD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260732
更新日期:1996-07-01 00:00:00
abstract::The glycosylphosphatidylinositol (GPI)-anchored CD59 protein (human protectin) protects cells against complement-induced lysis, binds to CD2 and also transduces activation signals within T cells. We have further examined the biochemical signals transduced by CD59 and addressed its role in regard to the CD3-mediated si...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250704
更新日期:1995-07-01 00:00:00
abstract::Vaccinia virus (VV) has been most commonly used as the vaccine to protect individuals against the causative agent of smallpox (variola virus), but it also uses a number of strategies meant to evade or blunt the host's antiviral immune response. Natural killer T (NKT) cells are a subset of immunoregulatory CD1d-restric...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636024
更新日期:2006-10-01 00:00:00
abstract::There is a current need for safe, cheap, and effective vaccine adjuvants, to combine with sub-unit antigens to enhance their immunogenicity. In this study we have used probiotic Bacillus subtilis spores, known to be safe and fully tolerated by ingestion in man, and explored their ability to influence the magnitude and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636875
更新日期:2007-06-01 00:00:00
abstract::We show that, in vitro, the response of thymocytes to certain stimuli, and their survival largely depend on the nature of the culture environment, i.e. whether thymocytes are stimulated within intact thymus lobes or in cell suspension. Exposure of isolated thymocytes to 12-O-tetra-decanoylphorbol 13-acetate (TPA)+iono...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221011
更新日期:1992-10-01 00:00:00
abstract::Two out of five murine IgG3 anti-trinitrophenyl (TNP) monoclonal antibodies (mAb) obtained either by immunization with TNP-keyhole limpet hemocyanin (KLH) (CB1, CB5, CB6 and 4H10) or with dinitrophenyl-lipopolysaccharide (9A6), exhibited anti-IgG rheumatoid factor (RF) activity (CB6 and 4H10). The anti-IgG activity of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191123
更新日期:1989-11-01 00:00:00
abstract::By combining velocity and linear density fractionations as well as target cell rosetting techniques we have isolated and morphologically identified the human effector cell type responsible for spontaneous, trypsin-augmentable cytotoxicity against chicken red cells and human myeloma cell line targets. This cell is a la...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830071104
更新日期:1977-11-01 00:00:00
abstract::About one hundred immunologists recently met in Capo Caccia, Sardinia, for the first of three events that will comprise the EMBO Conference Series, organised by the ENII, on Molecular and Cellular Mechanisms of Immune Regulation. The 2007 Conference focused on how immunity is shaped in healthy and diseased tissues. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200790031
更新日期:2007-08-01 00:00:00
abstract::To elucidate the mechanism of autoimmune disease in neonatally thymectomized (NTX) mice, we have investigated the responsiveness of the self-reactive T cells which have not undergone clonal deletion in such animals. Consistent with a recent report (Yuuki et al., Eur. J. Immunol. 1990. 20: 1475), T cells bearing V beta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210310
更新日期:1991-03-01 00:00:00
abstract::The diversity of cytokine production patterns displayed by T cells activated in vivo was investigated by analyzing short-term antigen-specific CD4+ T cell clones and single CD4+ T cells derived from draining lymph nodes of mice undergoing a T helper 2 (Th2)-like response to keyhole limpet hemocyanin (KLH). On average,...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250506
更新日期:1995-05-01 00:00:00
abstract::Delivery of foreign antigens to mucosal surfaces, such as the pulmonary airways, has been shown to preferentially induce Th2-mediated responses in humans and in mice. What is not clear from these studies is whether this preferential skewing in responses is the result of the limited types of antigen being administered ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200003)30:3<840::AID-IMMU840>3.0
更新日期:2000-03-01 00:00:00
abstract::The death of individual cells is a frequent and physiological event in the mammalian immune system and most often occurs by apoptosis. It is becoming increasingly clear that cell death is also induced during bacterial infections. Here we report that, in addition to the apoptotic form already established, a necrosis-li...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324582
更新日期:2004-05-01 00:00:00
abstract::In M54 mice transgenic for a completely rearranged mu(a) heavy chain there is a decrease in total B cells and the rearrangement of endogenous immunoglobulin genes is partially inhibited. Surprisingly, however, endogenous immunoglobulin gene rearrangement and significant heavy chain polypeptide production does occur. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220909
更新日期:1992-09-01 00:00:00
abstract::We studied the capacity of human factor H to promote the secretion of a lymphocyte-activating factor (LAF) by human monocytes cultured under serum-free conditions. Presence of LAF in the culture supernatants was assessed with the mouse thymocyte assay. Highly purified factor H alone had no effect on thymocyte prolifer...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210416
更新日期:1991-04-01 00:00:00
abstract::Using a new mAb, 2F1, we characterize a mouse natural killer (NK) cell antigen termed 'killer cell lectin-like receptor G1' (KLRG1; formerly mouse MAFA or 2F1-Ag). KLRG1 is expressed on 30-60% of murine NK cells, and a small fraction of T cells, and is composed of a homodimer of glycosylated 30-38-kDa subunits. Striki...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200003)30:3<920::AID-IMMU920>3.0
更新日期:2000-03-01 00:00:00
abstract::Subcutaneous immunization with hen egg-white lysozyme (HEL) in complete Freund's adjuvant induces, both in antibody responder and nonresponder mice, a classical delayed-type hypersensitivity (DTH) reaction evaluated as footpad swelling. This response can be specifically transferred to naive recipients by Lyt-1+2- T ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140910
更新日期:1984-09-01 00:00:00
abstract::In this report we investigated the production and role of interleukin (IL)2 and IL4 in the generation of antigen-specific cytotoxic T cells (CTL). We used as our model the ultraviolet-light-induced epithelial tumor 1591, a highly immunogenic regressor tumor which evokes a strong cell-mediated immune response leading t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210813
更新日期:1991-08-01 00:00:00