Abstract:
:Intrathymic deletion of immature thymocytes that express self-reactive TCR specificities is essential in the generation of self tolerance. Medullary thymic epithelial cells (mTEC) expressing the transcriptional regulator Aire play a key role in this process by regulating expression of tissue-restricted antigens to ensure tolerance to peripheral tissues. Here, we have analysed the cellular and molecular requirements for the initial appearance of Aire+ mTEC in the embryonic thymus, in addition to their persistence in the adult thymus. Analysis of thymic ontogeny shows that the emergence of embryonic Aire+ mTEC occurs prior to the appearance of mature thymocytes, and depends upon lymphoid tissue inducer cells expressing retinoic acid receptor-related orphan receptor gamma. In the adult thymus, we show that Aire+ mTEC develop in the absence of thymocyte positive and negative selection and CD40 signalling, but are present at reduced frequency. Collectively these data support a model where the initial differentiation of Aire+ mTEC involves receptor activator of NF-kappaB (RANK)-RANKL interactions with lymphoid tissue inducer cells, with subsequent mTEC turnover and/or survival involving CD40-mediated signalling following interactions with mature CD4+ thymocytes that express CD40L.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
White AJ,Withers DR,Parnell SM,Scott HS,Finke D,Lane PJ,Jenkinson EJ,Anderson Gdoi
10.1002/eji.200738052subject
Has Abstractpub_date
2008-04-01 00:00:00pages
942-7issue
4eissn
0014-2980issn
1521-4141journal_volume
38pub_type
杂志文章abstract::Pulmonary mucosal immune response is critical for preventing opportunistic Aspergillus fumigatus infections. Although fungus-specific CD4+ T cells in blood are described to reflect the actual host-pathogen interaction status, little is known about Aspergillus-specific pulmonary T-cell responses. Here, we exploit the d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948524
更新日期:2020-11-01 00:00:00
abstract::The specificity and efficiency of endogenous peptides in the HLA class I binding have been investigated by the use of a simple procedure that is based on the serological detection of the folding of HLA class I alpha chains that is induced by the binding to specific peptides in the presence of beta 2 microglobulin. HLA...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220834
更新日期:1992-08-01 00:00:00
abstract::Although most cells of the T cell receptor (TcR) gamma/delta lineage are CD4-CD8-, some gamma/delta cells express CD8. We show here that mouse thymic gamma/delta cells express CD8 following in vitro activation by concanavalin A. Staining with monoclonal and polyclonal antibodies to the CD8 alpha (Ly-2) and CD8 beta (L...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200431
更新日期:1990-04-01 00:00:00
abstract::NKp44 (NCR2) is a distinct member of natural cytotoxicity receptors (NCRs) family that can induce cytokine production and cytolytic activity in human NK cells. Heparan sulfate proteoglycans (HSPGs) are differentially expressed in various normal and cancerous tissues. HSPGs were reported to serve as ligands/co-ligands ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445177
更新日期:2015-04-01 00:00:00
abstract::We analyzed the avidity and CD28-mediated co-stimulatory requirements for the activation of T cells in vivo and in vitro. The strength of the T cell/antigen-presenting cell interaction was varied by using altered peptide ligands for stimulation. Co-stimulatory requirements were studied using T cells from CD28-deficien...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260908
更新日期:1996-09-01 00:00:00
abstract::The generation of effective type 1 T helper (Th1)-cell responses is required for immunity against intracellular bacteria. However, some intracellular bacteria require interleukin (IL)-17 to drive Th1-cell immunity and subsequent protective host immunity. Here, in a model of Mycobacterium bovis Bacille Calmette-Guerin ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141569
更新日期:2012-02-01 00:00:00
abstract::CSF1R (colony stimulating factor 1 receptor) is the main receptor for CSF1 and has crucial roles in regulating myelopoeisis. CSF1R can be proteolytically released from the cell surface by ADAM17 (A disintegrin and metalloprotease 17). Here, we identified CSF1R as a major substrate of ADAM17 in an unbiased degradomics ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646482
更新日期:2016-12-01 00:00:00
abstract::Cells of the innate immune system build immunological memory via epigenetic reprogramming after stimulations with microbial ligands. This functional readjustment allows for enhanced nonspecific inflammatory responses upon secondary challenges, a process termed "trained immunity." The epigenomic blueprint of trained mo...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201545497
更新日期:2016-04-01 00:00:00
abstract::To estimate the extent of the TRG gamma variable (V) gene repertoire used in human T cell ontogeny, we have analyzed the variety of V gamma gene rearrangements in a large series of T and non-T acute and chronic leukemias. A limited heterogeneity of rearranged fragments was observed: only 13 types of differently rearra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180126
更新日期:1988-01-01 00:00:00
abstract::Indirect immunofluorescence revealed that 13% of BALB/c and 33% of CBA spleen cells of B type carry specific binding sites at their surface for double-stranded poly(I).poly(C). Pretreatment of BALB/c spleen cells with anti-mouse immunoglobulin serum increased the number of B cells capable of binding poly(I).poly(C) in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081213
更新日期:1978-12-01 00:00:00
abstract::The class II transactivator (CIITA) has been referred to as the "master control factor" for the expression of MHC class II (MHCII) genes. As our knowledge on the specificity and function of CIITA grows, it is becoming increasingly evident that this sobriquet is entirely justified. First, despite extensive investigatio...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200424964
更新日期:2004-06-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSC) play an important role in the cellular network regulating immune responses in cancer, chronic infectious diseases, autoimmunity, and in other pathological conditions. Morphological, phenotypic and functional heterogeneity is a hallmark of MDSC. This heterogeneity demonstrates th...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201040895
更新日期:2010-11-01 00:00:00
abstract::As we approach the centenary of Elie Metchnikoff's Nobel Prize (1908), it is opportune to reflect upon the history of macrophage immunobiology, take stock of current knowledge and anticipate questions for the future. Starting from his appreciation of phagocytosis as an important determinant of host defence against inf...
journal_title:European journal of immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1002/eji.200737638
更新日期:2007-11-01 00:00:00
abstract::The activating NK cell receptor NKG2D binds to numerous stress-induced cell surface glycoproteins with MHC class I-related ectodomains. In humans, NKG2D ligands (NKG2DL) are members of the MHC-encoded MIC and non-MHC-encoded ULBP families of proteins. The redundancy of NKG2DL raises questions about unique features ass...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839074
更新日期:2009-06-01 00:00:00
abstract::Intravenous immunoglobulins (IVIgs) efficiently suppress a variety of autoimmune diseases. Over the past few years several potential mechanisms underlying this antiinflammatory activity have become apparent. Among these, terminal sialic acid residues in the sugar moiety of the immunoglobulin G constant fragment have b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242710
更新日期:2012-12-01 00:00:00
abstract::Evidence is presented that the phenotype of the rat T helper cells is stable after priming and boosting with a hapten-carrier conjugate and that the phenotype is W3/25+, MRC OX-22-, MRC OX-8-. Data are also presented that demonstrate the presence of doubly marked, W3/25+, MRC OX-8+, cells in the spleens of rats primed...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130916
更新日期:1983-09-01 00:00:00
abstract::T cell recognition of self antigens is a key event in the pathogenesis of autoimmune diseases. To date, the initial events that trigger autoreactive T cells are unknown. The "molecular mimicry" hypothesis predicts that during an infection T cells that recognize both a microbial antigen and a related self peptide becom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<448::AID-IMMU448>3.0
更新日期:2000-02-01 00:00:00
abstract::The anti-PC antibodies of BALB/c origin bear predominantly the idiotype characteristic of the phosphorylcholine (PC)-binding T15 idiotype than sera from adult mice, and, unlike the latter, they also contain detectable amounts of anti-T15 antibodies. By 2 weeks of age the anti-T15 antibodies are no longer detectable wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060710
更新日期:1976-07-01 00:00:00
abstract::The development of preventive or therapeutic recombinant vaccines and the generation of serodiagnostic assays for infectious diseases depend essentially on the availability of molecularly defined antigens. A major bottleneck for the identification of suitable target antigens for many pathogens is the isolation of suff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535538
更新日期:2006-04-01 00:00:00
abstract::By means of a novel technique for identification and isolation of MHC class II-restricted antigen-specific T cells, we describe here in non-transgenic BALB / c mice physiological positive selection of an oligoclonal population of T cells which recognizes both a self MHC-derived peptide (Ialpha52) and a bacterial homol...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<3826::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::A previously designed isotopic in vivo method for measuring delayed hypersensitivity was used with nonantigenic (or weakly antigenic) phlogogenic substances. A strong reaction was observed in some circumstances, witnessing the fact that the method has a specific and a nonspecific component. The reaction is drastically...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060606
更新日期:1976-06-01 00:00:00
abstract::Chlamydia pneumoniae stimulates potently maturation of and cytokine secretion by bone marrow-derived dendritic cells (BMDDC). BMDDC responses depend mainly on Toll-like receptor (TLR)2 and to a minor extent on TLR4. We demonstrate here using C. pneumoniae in an infectious model with the replication-permissive epitheli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200209)32:9<2460::AID-IMMU2460>3
更新日期:2002-09-01 00:00:00
abstract::The development of the antibody repertoire in newborn mice is greatly influenced by idiotype network interactions. It has been demonstrated that anti-idiotypic antibodies either directly injected or transferred from the mother may alter the repertoire for life. For an elucidation of the underlying mechanisms we have a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241216
更新日期:1994-12-01 00:00:00
abstract::Cytokines are the main agents known to regulate Th1 / Th2 commitment, where they may operate through paracrine activity within clusters of T cells gathered around dendritic cells (DC). An in vitro system is used here to test this possibility, using clusters around DC composed of naive TCR-transgenic ovalbumin peptide ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200005)30:5<1255::AID-IMMU
更新日期:2000-05-01 00:00:00
abstract::Infection with parasitic worms (helminths) alters host immune responses and can inhibit pathogenic inflammation. Helminth infection promotes a strong Th2 and T regulatory response while suppressing Th1 and Th17 function. Th2 responses are largely dependent on transcriptional programs directed by Stat6-signaling. We ex...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848072
更新日期:2020-10-16 00:00:00
abstract::We have examined the influence of listeriolysin O (LLO), the hemolysin secreted by the pathogenic bacterium Listeria monocytogenes, on major histocompatibility complex class II-dependent T cell activation. Stimulation of T cells by native antigens but not by peptides is inhibited upon pretreatment of antigen-presentin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270716
更新日期:1997-07-01 00:00:00
abstract::We have investigated the vaccine potential of two peptides derived from the 6-kDa early secretory antigenic target (ESAT)-6 antigen in the mouse model of tuberculosis. The peptides were both strongly immunogenic in B6CBAF1 (H-2b,k) mice and primed recall responses of the same intensity after immunization. However, bot...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200006)30:6<1724::AID-IMMU1724>3
更新日期:2000-06-01 00:00:00
abstract::The expression of IgG Fc receptor (FcR) molecules was examined on Ly-1+ B cells and B cells tumors. FcR molecules were found on a representative Ly-1+ B cell lymphoma of the pre-B and as well as of the mature cell B phenotypes. The expression of the FcR did not change on these cells during their differentiation to B c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170621
更新日期:1987-06-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) is acquired by neonates through milk and first infects lymphocytes in Peyer's patches. We show here that newborn mice lacking beta7 integrin or L-selectin were infected with MMTV at wild-type levels in both their lymphoid and mammary tissues. Superantigen-mediated activation and cognat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<945::AID-IMMU945>3.0
更新日期:2002-04-01 00:00:00
abstract::The thymic medulla is composed of distinct epithelial cell subsets, defined in this report by the reactivity of two novel antibodies, 95 and 29, raised against mouse thymic epithelial cell lines. These antibodies were used to probe the development of medulla in wild-type or mutant thymuses. In CD3 epsilon-deficient mi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270615
更新日期:1997-06-01 00:00:00